TRPV4 | Transient Receptor Potential Cation Channel Subfamily V Member 4

Family:
Transient receptor potential channels

Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN

Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations

TRPV4: Background information

TRPV4 is a non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity exhibiting an outward rectification. It is activated by exposure to hypotonicity within the physiological range as well as by heat, low pH, citrate and phorbol esters. An increase of intracellular Ca²⁺ potentiates currents. The channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism.

Gene:
TRPV4

Human Protein:
UniProt Q9HBA0

Tissue:
Trachea, kidney, liver, spleen, skin, brain, PNS, heart

Function/ Application:
TRPV4 has been found to be involved in multiple phsiological functions, as the regulation of systemic osmotic pressure in the brain, the skin barrier, pain, osmosensation, homeostasis. In the vascular endothelium, it contributes to intracellular Ca²⁺ homeostasis and regulation of cell volume while responding to mechanical stresses on the vasculature. It is crucially for the function of the cardiac, respiratory, urinary, skeletal, and digestive systems, as well as in the central and peripheral nervous systems.

Pathology:
Brachyolmia 3 (BCYM3), Spondylometaphyseal dysplasia Kozlowski type (SMDK), Metatropic dysplasia (MTD), Neuronopathy, distal hereditary motor, 8 (HMN8), Charcot-Marie-Tooth disease 2C (CMT2C), Scapuloperoneal spinal muscular atrophy (SPSMA), Spondyloepiphyseal dysplasia Maroteaux type (SEDM), Parastremmatic dwarfism (PSTD), Digital arthropathy-brachydactyly, familial (FDAB), Avascular necrosis of the femoral head, primary 2 (ANFH2)

Interaction:
Calmodulin, Map7, Src family Tyr protein kinases (LYN, SRC, FYN, HCK, LCK and YES), CTNNB1, PACSIN1, PACSIN2, PACSIN3, ITPR3, AQP5, ANO1, PKD2, and is part of the complex containing containing MLC1, AQP4, HEPACAM and ATP1B1.

Modulator:
Cannabidiol, Resiniferatoxin, GSK1016790A, RN1747, 4α-PDD, GSK2193874, HC067047, La³⁺, Gd³⁺, ruthenium red

Assays:
Patch Clamp: whole cell, temperature control

Recommended Reviews:
International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003
TRPV4: Molecular Conductor of a Diverse Orchestra, Physiological Reviews 96(3):911-973 John P.M White et al. 2016

Application Notes

TRPV4 - Activation and inhibition of TRPV4 using different stimuli on the Port-a-Patch

Port-a-Patch application note: (3.79 MB)

preview the file here for download

Publications

2022- The discovery of (1R, 3R)-1-(3-chloro-5-fluorophenyl)-3-(hydroxymethyl)-1,2,3,4-tetrahydroisoquinoline-6-carbonitrile, a potent and selective agonist of human transient receptor potential cation channel subfamily m member 5 (TRPM5) and evaluation of

SyncroPatch 384 publication in Bioorganic & Medicinal Chemistry (2022)

Authors:
Davies M.R., Martinec M., Walls R., Schwarz R., Mirams G.R., Wang K., Steiner G., Surinach A., Flores C., Lave T., Singer T., Polonchuk L.

2021 - Glucosylsphingosine evokes pruritus via activation of 5-HT2A receptor and TRPV4 in sensory neurons

Port-a-Patch Publication in British Journal of Pharmacology (2021)

Authors:
Sanjel B., Kim B-H., Song M-H., Carstens E., Shim W-S.

2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

Icon Orbit Mini Orbit mini publication in Cells (2019)

Authors:
Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.

2018 - Electrophysiological experiments in microgravity: lessons learned and future challenges

Port-a-Patch publication in Microgravity (2018)

Authors:
Wuest S.L., Gantenbein B., Ille F.,Egli M.

2018 - Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

Vesicle Prep Pro publication in Scientific Reports (2018)

Authors:
Startek J.B., Talavera K., Voets T., Alpizar Y.A.

2012 - Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells

Port-a-Patch publication in Histochemistry and Cell Biology (2012)

Authors:
Mergler S., Garreis F., Sahlmüller M., Lyras E.-M., Reinach P.S., Dwarakanath A., Paulsen F., Pleyer U.

TRPV4