31.03.2022 - A deep-dive into TRPM8
TRPM8 | Transient Receptor Potential Cation Channel M8
An advanced temperature control system is standard in the SyncroPatch 384 system, enabling precise control of the temperature (in the range of 10-37°C) at the measurement site and at 12 different deck positions.
- Stable temperatures of cells, patch clamp module and solutions
- A stable recording temperature allows for stable currents
- The temperature control allows for heating or cooling
The figures below show temperature regulated action - TRPM8 currents at 12°C or higher temperatures. In general, TRPM8 is activated at lower temperatures (< 25°C). Measurement site, cells and solutions can be accurately temperature controlled.
Fig. 1 Screenshots of TRPM8 recordings showing channel activity at 12°C. Left: raw current traces and right: Current amplitude over time proving current stability. CHO-hTRPM8 were kindly provided by Charles River.
Fig. 2 Measurement site, cells and solutions can be accurately temperature controlled – suits any target, e.g. cold regulated TRP channels like TRPM8 (CHO-hTRPM8 kindly provided by Charles River).
TRPM8: Background information
TRPM8, also known as the cold and menthol receptor 1 (CMR1) is the primary transducer of cold somatosensation in humans. The TRPM8 channel is a homotetramer, composed of four identical subunits with a transmembrane domain with six helices (S1–6). TRPM8 is an ion channel, upon activation it allows the entry of Na+ and Ca2+ ions to the cell that leads to depolarization and the generation of an action potential and it is activated by cold temperatures and cooling agents, such as menthol and icilin.
Sensory neurons, dorsal root ganglia neurons, prostate, lung, bladder, prostatae tumors
Temperature transducer for ‘‘cold’’, proliferation, generation of an action potential
Cancer, thermoception, neuropathic pain, migrane, dentin sensitivity
TCAF1, PIP2, Gαq, Protein phosphatase 1, Ubiquitin specific peptidase 10
Cilin, eucalyptol, menthol, capsaicin, ethanol, linalool, geraniol, capsazepine , BCTC, cannabidiol
Patch Clamp: whole cell, room temperature, State- and use-dependence
TRPM8 is a ligand-gated, intracellular pH- and thermo-sensitive ion channel
Recommended Reviews:International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003
Data and Applications