Murine Mammary Carcinoma Cells - Effect of CAF (cyclophosphamide, adriamycin (doxorubicin) and 5-fluouracil)
Murine mammary carcinoma cells (H8N8 and H8N8 T3.2) were monitored over a time period of 500 h. Changes in impedance, and therefore confluency, were used as a measure of toxicity.
H8N8 cells have been developed as a tool to investigate tumor cell recurrence, chemoresistance and epithelial-to-mesenchymal transition (EMT). Cells were treated with increasing concentrations of CAF (cyclophosphamide, adriamycin (doxorubicin) and 5-fluouracil) on the CardioExcyte 96 during impedance measurements. Therefore, the CardioExcyte 96 in combination with murine mammary carcinoma cells provides a novel tool for investigating therapy resistance of cancer cells in vitro.
Data kindly provided by Oliver Reinhardt, MPI Experimentelle Medizin, Göttingen