Orbit 16 - Painting法の苦痛から解放されます

ナニオンの Orbit 16（オービット16）のプラットフォームは、16 chで脂質二分子膜の迅速な全自動の同時作製、完全同時測定を実現し、脂質二分子膜実験における painting法の苦痛から研究者を解放します。 Orbit 16 は以下の優位性を有しています：

16 ch の脂質二分子膜をボタン一つで自動作製
低ノイズ、高帯域幅での完全同時測定
16 ch同時測定による連続データ生成
低ノイズの16 chアンプまたは外部接続アンプの統合が可能
専用の総合ソフトウェア同梱
標的チャネルタンパク質を直接再構成または、プロテオリポソームで膜融合
イオンチャネル：電位依存性、リガンド依存性、温度依存性
ナノポア、抗菌ペプチド、トキシンなど...
ティスポ式 MECA 16 チップによる低コスト実験

Orbit 16 は専用の低ノイズ16 chアンプ (Elements社 s.r.l.) を標準モデルで搭載し、広帯域幅での16chの脂質二分子膜の完全同時測定を容易に実現しています。さらに、Orbit 16はTecella社製のTriton 16chアンプなど、多くの市販アンプとの接続に対応しています。EPC-10 や Axopatchなどの1chアンプとの接続では、Orbit に内蔵された多重化スイッチボードにより測定サイトを次々に自動切替えして測定を行うことが可能です。

Orbit 16 バイレイヤー用プラットフォーム構成

Orbit 16 コンプリートシステムは、レコーディングステーション、内蔵型のElements社製 e16 バイレイヤーアンプ、Ionera社製のMECA 16 チップ専用の測定モジュールで構成されています。

詳細情報：

ソフトウェア

Orbit 16 EDR Software

eFOUR Software

The software for the Orbit 16 was developed by our partner Elements SRL (Italy). It is intuitive and easy to learn. Please watch this video on the Orbit mini (contains the same software package as the Orbit 16) to learn about the software:

消耗品

MECA 16

The MECA 16 recording substrate contains a 4 x 4 array of circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is automatically formed by remotely actuated painting (Ionera1-SPREAD), thus roofing the liquid-filled cavity. The bilayers can be easily and repeatedly zapped and re-formed in an automated fashion. After bilayer formation, ion channels or nanopores are reconstituted via self-insertion, proteoliposome fusion or dilution from detergent micelles.

The MECA recording chips are produced and quality assured by our partner Ionera Technologies GmbH in Freiburg Germany and shipped from Munich to our international customers. Different types of MECA 16 chips are available depending on the sample.

Orbit 16 Meca Chip

Available chip types

"Meca 16 Recording Chips 50 µm": 16-well recording chip with 50 µm cavity size (Order # 131002)
"Meca 16 Recording Chips 16 µm": 16-well recording chip with 16 µm cavity size (Order # 131003)

インタビュー & ケーススタディ

Prof. Dr. Friedrich Simmel - Statement about the Orbit 16

Icon Orbit “The Orbit 16 enables us to generate high quality, single channel recordings with synthetic DNA membrane channels, which in our experience are notoriously difficult to measure. DNA pores are quite hard to functionally incorporate into lipid bilayers, but could be successfully investigated using the Orbit 16, as published in Science. The Orbit 16 offers a drastic increase in throughput since it substantially speeds up formation of bilayers and data generation by its parallel recording channels, thus providing us an easy-to-use platform for efficient and accurate research on DNA nanodevice-membrane interactions.”

Dr. Friedrich Simmel, Professor, Systems Biophysics and Bionanotechnology, Physics Department and ZNN/WSI
Technical University of Munich, Munich, Germany

Prof. Dr. Stefan Howorka - Statement about the Orbit 16

Icon Orbit “Within our research on the CsgG channel, Nanion’s Orbit 16 - combined with Ionera's MECA Chip technology - has substantially boosted our scientific output. The outstanding research tool is easy to handle and speeds up the parallel generation of 16 bilayers. By increasing the throughput of single-channel current recordings, it is a breakthrough in the biophysical analysis of pore forming proteins. Within approximately one week’s worth of lab time, we had the data needed for the recent paper in Nature. This would have been hard to achieve using conventional serial bilayer methods. In addition, the ease-of-use provided by the Orbit 16 shortens the learning curve for making high quality bilayer recordings. As a benefit in academia, students can now get hands-on experience with bilayer recordings also for shorter projects.“

Dr. Stefan Howorka, Associate Professor of Organic Chemistry and Chemical Biology
University College London, London, UK

データ＆アプリケーション

Alpha-Hemolysin - Parallel Recordings of monoPEG-28 Block

Icon Orbit Orbit 16 data and applications:
Data courtesy of Dr. Gerhard Baaken et. al., University of Freiburg / Ionera.

Event-averaged histograms (black) and overlaid current traces (blue) of parallel and simultaneous recordings on a MECA chip of monoPEG-28-mediated blockages of hemolysin nanopore(s). The current traces were recorded with a multichannel amplifier (Tecella Jet 16). Histograms were derived from the mean current levels of at least 2000 visits of blocked stated per cavity (20 kHz sample frequency).
Read the full paper. (Am. Chem. Soc Nano, 5(10), 8080-8088, 2011)

Alpha-Hemolysin - Block by Mono- and Poly PEGs

Icon Orbit Orbit 16 data and applications:
Data courtesy of Dr. Gerhard Baaken et.al, University of Freiburg / Ionera.

Current traces and histograms derived from recordings of αHL pores blocked by monoPEG-28 and polyPEG-1500 on an Ionera MECA chip (AxoPatch 200B, filter freq: 20kHz, digitized at 200 kHz).
Read the full paper: (Am. Chem. Soc Nano, 5(10), 8080-8088, 2011)

Alamethicin - Parallel recordings

Icon Orbit Orbit 16 data and applications:
Data courtesy of Dr. Gerhard Baaken, University of Freiburg / Ionera.

The data image shows parallel recordings from reconstituted alamethicin channels. See also the paper: "Alamethicin Supramolecular Organization in Lipid Membranes from ¹⁹F Solid-State NMR", Salnikov et al. (2016) Biophysical Journal 111(11): 2450-2459.