• Orbit 16 TC

    fully parallel recording of 16 bilayers

2022 - Candidalysins Are a New Family of Cytolytic Fungal Peptide Toxins

Icon Orbit  Orbit 16 publication in mBio (2022)

Richardson J. P., Brown R., Kichik N., Lee S., Priest E., Mogavero S., Maufrais C., Wickramasinghe D. N., Tsavou A., Kotowicz N. K., Hepworth O. W., Gallego-Cortés A., Ponde N. O., Ho J., Moyes D. L., Wilson D., D’Enfert C., Hube B., Naglik J. R.

mBio (2022) doi: 10.1128/mbio.03510-21


Candidalysin is the first cytolytic peptide toxin identified in any human fungal pathogen. Candidalysin is secreted by Candida albicans and is critical for driving infection and host immune responses in several model systems. However, Candida infections are also caused by non-C. albicans species. Here, we identify and characterize orthologs of C. albicans candidalysin in C. dubliniensis and C. tropicalis. The candidalysins have different amino acid sequences, are amphipathic, and adopt a predominantly α-helical secondary structure in solution. Comparative functional analysis demonstrates that each candidalysin causes epithelial damage and calcium influx and activates intracellular signaling pathways and cytokine secretion. Importantly, C. dubliniensis and C. tropicalis candidalysins have higher damaging and activation potential than C. albicans candidalysin and exhibit more rapid membrane binding and disruption, although both fungal species cause less damage to epithelial cells than C. albicans. This study identifies the first family of peptide cytolysins in human-pathogenic fungi.

Download here


We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.