Orbit mini - プラグ＆ペイント
Orbit mini（オービットミニ）は、超小型のバイレイヤーワークステーションです。人工脂質二重膜を用いた実験における、脂質膜の形成／膜タンパク質の再構成／単一チャネル電流の測定を簡単に行うことができます。薬物作用の評価や膜タンパク質の機能・構造解析に最適です。
Orbit miniの特徴と優位性は以下の通りです。
- スタンドアローンの超小型ターンキーシステム
- 4ch の低ノイズアンプ内蔵
- 温度コントロール可能（オプション） - 自動冷却 & 加熱
- 最高のバンド幅による低ノイズ測定
- 4ch 同時測定によるスループット向上
- 専用の記録ソフトウェア同梱
- ターゲット分子は直接導入、または（プロテオ）リポソームとして融合
- イオンチャネル：電位依存性 & リガンド依存性、温度依存性
- ナノポア、抗菌ペプチド、トキシンなど...
- MECAチップによる低コスト実験
Orbit mini は超小型の4chアンプを内蔵し、高いバンド幅による低ノイズ測定を実現しました。特別な追加設備は必要なく、どこでも脂質平面膜実験が行えます。さらに Orbit mini 独自のシステム設計により、測定チャンバー内の温度制御においてノイズ発生がありません。
Orbit mini の完全なプラットフォームは、4chアンプ（Elements社 s.r.l.）内蔵のレコーディングユニット、コンピュータ制御で測定チャンバーの動的冷却及び加熱を行う温度コントロールユニットから構成されます。Ionera社の MECA 4 ディスポ式測定チップはペインティング法による平面膜形成と4箇所の脂質平面膜の同時記録に使用します。
詳細情報:
温度コントローラは冷却および加熱機能を搭載しており，チップの温度を 0℃～50℃の範囲で制御できます。
Orbit miniの温度制御実験例: α-ヘモリシンのポアに対するPEGによる阻害
(A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature.
The image war kindly provided by Ionera Technologies GmbH
Orbit miniと蛍光顕微鏡を使用した実験には "蛍光顕微鏡取付キット" を使用します。キットには，下記対物レンズに適合するファラデーシールドと蛍光顕微鏡用のキャビティーサイズ 150 µmの "MECA4 FLUOチップ（製品コード 13 2004）"が同梱されています。その他の対物レンズへの対応状況はお問い合わせ下さい。
Orbit mini 温度コントローラ（製品コード: 12 2002） The MECA 4 OPTO-technology enables simultaneous optical and electrical recordings in free standing artificial lipid bilayers.
Orbit mini用のソフトウェアは、パートナー会社である Elements SRL社 (イタリア)で開発されました。直感的な操作で簡単にお使いいただけます。Orbit miniのソフトウェアの詳細は、こちらの動画をご覧下さい：
EDR ソフトウェアの更新は Elements s.r.l 社のホームページからダウンロードして下さい。 Click here for Software downloads.
Orbit miniの実験に使用するMECA4チップには、不活性化ポリマーに2x2配列で円形マイクロキャビティーが配置されています。各キャビティーにはAg/AgCl微小電極が独立して統合されています。MECA4チップはpainting法によりファンクショナルな脂質二分子膜を高確率で形成可能で、グラミシジン、α-ヘモリシン、Kv1.3、Navなど、多岐に渡るイオンチャネルの包埋において既にバリデートされています。
MECA測定チップは、パートナー会社であるIonera Technologies GmbH社（ドイツ・フライブルク）で製造、品質管理がされ、ドイツミュンヘンから世界のユーザーへ発送されます。アプリケーションに合わせて、各種のMECAチップから最適なチップお選びいただけます。
Prof. Dan Burden “The compactness of the instrument makes for flexible and convenient placement in the lab. With a tiny footprint, it sits on a table or desk accompanied by only a laptop. In addition, bilayers are painted 'blind' with a teflon wand supplied by Nanion without observing the partition through a microscope. Thus, the Source: "Getting going with the Orbit mini planar bilayer system: A narrative" From: Temperature control of the Orbit mini: PEG induced current blockages of a alpha-hemolysin pore (A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature. Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram. Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram. (A) Effect of temperature on TRPA1 activity The Arrhenius plot of the same data resulted in a Q10 of 46 (Literature: Q10 ~ 40). Current recordings of single VDA channels from selected bilayers in parallel. (A) Representative parallel recording from 3 VDAC channels in DPhPC membranes. Conditions: 1 M KCl, 25 mM Tris, pH 7,5, voltage change protocol is given below. Purified VDAC sample is a generous gift from Prof. Stephan Nussberger to Ionera, Department Biophysics, University of Stuttgart, Germany Presenter: Short introduction how to prepare membranes on the Orbit mini Date: September 17. 2020, 4:00 PM CET (10:00 AM EDT) Speakers: Dr. Conrad Weichbrodt (Senior Scientist / Product Manager Orbit family; Nanion Technologies) Learn about single channel measurements in bilayer recording using the orbit instrument family - Simplifying artificial bilayer experiments: Single-molecule experiments on micro-cavity arrays オプション ＆ 特徴
Orbit mini 温度コントローラ
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.
Orbit mini 蛍光顕微鏡取付キット
【Zeiss】 プラン-アポクロマート対物レンズ
W Plan Apochromat 20x（NA1.0, WD2.4）
W Plan Apochromat 40x（NA1.0, WD2.5）
W Plan Apochromat 64x（NA1.0, WD2.1） など
【Olympus】 UIS2対物レンズ（ノーカバー水浸）
UMPLFLN10XW（NA0.3, WD3.5）
XLUMPLFLN20XW（NA1.0, WD2.0）
LUMPLFLN60XW（NA1.0, WD2.0） など
【Nikon】 対物レンズ（水浸）
CFI Fluor 60×W（NA1.0 WD2.0）
CFI Apo 60×W NIR（NA1.0 WD2.8）
CFI Plan 100×W（NA1.1 WD2.5） など
Orbit mini オプション 製品ラインナップ
Orbit mini ラップトップPC（製品コード: 12 2003）
Orbit mini リアルタイム統計解析ソフトウェア（製品コード: 12 2004）
Orbit mini 蛍光顕微鏡取付キット（製品コード: 12 2005）
Orbit mini OPTO-INV product sheet 
(0.43 MB)
The MECA 4 OPTO-INV kit is comprised of an adapted Faraday shield for Nanion‘s Orbit mini bilayer recording station and a tailor-made holder for Ionera‘s MECA 4 OPTO-INV chips.
The holder allows for the positioning of the recording chips over the objective of an inverse microscope while enabling the electrical connection between the chips and the amplifier inside the Orbit mini.ソフトウェア
Orbit mini EDR ソフトウェア
ソフトエアの更新：
消耗品
MECA4 チップ
MECA4 チップ ラインナップ
インタビュー & ケーススタディー
Prof. Dan Burden - MECA 4 OPTO-INV
"The MECA OPTO system easily coupled to my inverted microscopes and worked with a wide range of objectives. The MECA OPTO-holder provides traditional convenient bilayer formation and channel insertion with good electrical noise characteristics while markings make locating microwells quick and easy. Simultaneous optical and electrical interrogation of the microwell aperture accelerates lipid bilayer validation for ion channel measurements.
Chemistry Department
Wheaton College
Prof. Dr. Chris Miller - Case Study about the Orbit mini
"Contrary to my initial expectations, the Orbit mini (red arrow) has performed exceptionally well for
our application, with vastly improved signal-tonoise characteristics over our best recordings on the home-built system."
entire support-package of microscope, illuminator, Faraday cage, vibration-table, and rig-rack is eliminated. The acquisition software is intuitive and robust, and the data can be stored in formats readable by the Axon analysis programs that we routinely use. Moreover, the four electrically independent holes in the chip-partition means that four bilayers can be monitored simultaneously, thus quadrupling the probability of catching a clean single channel to record. This represents a significant, practical boost in experimental efficiency.”
Prof. Dr. Chris Miller, Professor of Biochemistry, Investigator, Howard Hughes Medical Institute Member, US National Academy of Sciences, Brandeis University, Waltham, Massachusetts, USAデータ ＆ アプリケーション
Alpha-Hemolysin - Temperature Control
Orbit mini and applications:
Data were kindly provided by Ionera.
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.
OccK1 - Outer Membrane Protein of the Pathogenic Bacterium Pseudomonas Aeruginosa
Orbit mini and applications:
Data were kindly provided by Ionera.
Conditions: 150 mM KCl, 5 mM MES, pH 6, 10 mM enrofloxacin, + 60 mV
OmpF - Current block
Orbit mini and applications:
Data were kindly provided by Ionera.
Conditions: 150 mM KCl, 5 mM HEPES, 5 mM MES, pH 6, 10 mM enrofloxacin, +60 mV
TRPA1 - Temperature Dependency
Orbit mini data and applications:
(B) The open probability (Po) versus the temperature and fitted with Boltzmann equation (EC50 was found at 14˚C).
VDAC - Application of Fluoxetine
Orbit mini and applications:
Data were kindly provided by Ionera.
VDAC was reconstituted in DPhPC membranes and partially blocked upon the addition of Fluoxetine (Prozac).
(B) Current trace illustration partial blocade of VDAC by Fluoxetine (Prozac).
(C) Current-voltage trace of a single VADC channel under a ramping voltage of -70/+70 mV before (green) and after (blue) addition of 50 µm Fluoxetine製品とチュートリアルの動画
2018 - Simplifying artificial bilayer experiments: Single-molecule experiments on micro-cavity arrays
Orbit 16 and Orbit mini Oral Presentation
Dr. Conrad Weichbrodt, Product Manager Orbit instrument family, Nanion Technologies GmbH, Germany
Source:
Webinar: "Artificial Lipid Bilayers in focus: Hand-held DNA-sequencing and biosensing with nanopores", June 28, 2018
2015 - Orbit mini Membrane Preparation Tutorial Video
Orbit Miniウェビナー
17.09.2020 | Webinar: Electrophysiological investigation of integral membrane proteins using the Orbit mini
Orbit mini Webinar
28.06.2018 | Webinar: Artificial Lipid Bilayers in focus: Hand-held DNA-sequencing and biosensing with nanopores
Orbit mini and Orbit 16
- Hand-held DNA-sequencing and biosensing with nanopores
27.01.2016 | Webinar: Instant bilayers - just add protein
Orbit 16 and Orbit Mini
This webinar covers the use of the lipid bilayer platforms from Nanion: the Orbit16 and the Orbit mini for characterization of membrane proteins like ion channels, bacterial porins and biological nanopores. Both bilayer systems support high quality low noise recordings, but differ in throughput capabilities and experimental features. The Orbit16, introduced in 2012 is a device for efficient formation of 16 lipid bilayers simultaneously, allowing for parallel bilayer-reconstitution of ion channels and nanopores.
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製品カタログ
Orbit mini OPTO- INV - Product Sheet
Orbit mini OPTO-INV product sheet (0.43 MB)
Orbit mini Product Flyer - Temperature Control
Orbit mini Product flyer (0.4 MB)簡易マニュアル
Orbit mini - Quickstart Guide "Getting Started"
Orbit mini Quickstart Guide "Getting Started"論文
2022 - Triggered Assembly of a DNA-Based Membrane Channel
Orbit Mini Publication in American Chemical Society (2022)
Lanphere C., Ciccone J., Dorey A., Hagleitner-Ertuğrul N., Knyazev D., Haider S., Howorka S.
2022 - Pore-forming moss protein bryoporin is structurally and mechanistically related to actinoporins from evolutionarily distant cnidarians
Orbit mini Publication in Journal of Biological Chemistry (2022)
Šolinc G., Švigelj T., Omersa N., Snoj T., Pirc K., Znidaršič N., Yamaji-Hasegawa A., Kobayashi T., Anderluh G., Podobnik M.
2022 - Photoactivation of a Mechanosensitive Channel
Orbit mini Publication in Frontiers in Molecular Biosciences (2022)
Crea F., Vorkas A., Redlich A., Cruz R., Shi C., Trauner D., Lange A., Schlesinger R., Heberle J.
2022 - Opening of glutamate receptor channel to subconductance levels
Orbit Mini Publication in Nature (2022)
Yelshanskaya M.V., Patel D.S., Kottke C.M., Kurnikova M.G., & Sobolevsky A.I.
2022 - Integrative Study of the Structural and Dynamical Properties of a KirBac3.1 Mutant: Functional Implication of a Highly Conserved Tryptophan in the Transmembrane Domain
Orbit Mini Publication in Molecular Biophysics (2022)
Fagnen C., Bannwarth L., Oubella I., Zuniga D., Haouz A., Forest E., Scala R., Bendahhou S., De Zorzi R., Perahia D., Vénien-Bryan C.
2022 - Highly shape- and size-tunable membrane nanopores made with DNA
Orbit 16 Orbit mini and 
Vesicle Prep Pro publication in Nature Nanotechnology (2022)
Xing Y., Dorey A., Jayasinghe L., Howorka S.
2022 - Emerging enterococcus pore-forming toxins with MHC/HLA-I as receptors
Orbit Mini Publication in Cell (2022)
Xiong X., Songhai Tian S., Yang P., Lebreton F., Bao H.,Sheng K., Yin L., Chen P., Zhang J., Qi W., Ruan J., Wu H., Chen H., Breault D.T., Wu H., Earl A.M., Gilmore M.S., Abraham J., Dong M.
2022 - Cryo–electron microscopy unveils unique structural features of the human Kir2.1 channel
Orbit Mini Publication in Science Advances (2022)
Fernandes C., Zuniga D., Fagnen C., Kugler V., Scala R., Péhau-Arnaudet G., Wagner R., Perahia D., Bendahhou S., Vénien-Bryan C.
2022 - Circularized fluorescent nanodiscs for probing protein–lipid interactions
Orbit mini Publication in Communications Biology (2022)
Ren Q., Zhang S., Bao H.
2022 - An oomycete NLP cytolysin forms transient small pores in lipid membranes
Orbit mini and 
Vesicle Prep Pro publication in Science Advances (2022)
Pirc K., Clifton L.A., Yilmaz N., Saltalmacchiamo A., Mally M., Snoj T., Žnidaršič N., Srnko M., Borišek J., Parkkila P., Albert I., Podobnik M., Numata K., Nürnberger T., Viitala T., Derganac J., Magistrato A., Lakey J.H., Anderluh G.
2022 - A reversibly gated protein-transporting membrane channel made of DNA
Orbit Mini Publication in Nature Communications (2022)
Dey S., Dorey A., Abraham L., Xing Y., Zhang I., Howorka S., Yan H.
2022 - A chip-based array for high-resolution fluorescence characterization of free-standing horizontal lipid membranes under voltage clamp
Orbit mini OPTO-INV Publication in Lab on a Chip (2022)
Ensslen T., Behrends J.C.
2021 - Structure and desensitization of AMPA receptor complexes with type II TARP γ5 and GSG1L
Orbit Mini Publication in Molecular Cell (2021)
Klykov O., Gangwar S. P., Yelshanskaya M. V., Laura Yen., Sobolevsky A. I.
2021 - Saccharomyces cerevisiae as a superior host for overproduction of prokaryotic integral membrane proteins
Orbit Mini Publication in Current Research in Structural Biology (2021)
Spruce Preisler S., Wiuf A.D., Friss M., Kjaergaard L., Hurd M., Ramos Becares E., Nurup C.N., Bjoerkskov F.B., Szathmary Z., Gourson P.E., Calloe K., Klaerke D.A., Gotfryd K., Pedersen P.A.
2021 - Rapid and multiplex preparation of engineered Mycobacterium smegmatis porin A (MspA) nanopores for single molecule sensing and sequencing
Orbit Mini Publication in Chemical Science (2021)
Yan S., Wang L., Du X., Zhang S., Wang S., Cao J., Zhang J., Jia W., Wang Y., Zhang P., Chen H-Y., Huang S.
2021 - Design, assembly, and characterization of membrane-spanning DNA nanopores
Orbit 16 and Orbit mini publication in Nature Protocols (2021)
Lanphere C., Offenbartl-Stiegert D., Dorey A., Pugh G., Georgiou E., Xing Y., Burns J.R., Howorka S.
2021 - ATP and large signaling metabolites flux through caspase-activated Pannexin 1 channels
Orbit mini publication in eLife (2021)
Narahari A.K., Kreutzberger A JB., Gaete P.S., Chiu Yu-Hsin, Leonhardt S.A., Medina C.B., Jin X., Oleniacz P.W., Kiessling V., Barrett P.Q., Ravichandran K.S., Yeager M., Contreras J.E., Tamm L.K., Bayliss D.A.
2020 - Structure and mechanism of bactericidal mammalian perforin-2, an ancient agent of innate immunity
Orbit mini and Vesicle Prep Pro publication in Science Advances (2020)
Ni T., Jiao F., Yu X., Aden S., Ginger L., Williams S.I., Bai F., Pražák V., Karia D., Stansfeld P., Zhang P., Munson G., Anderluh G.,Scheuring S., Gilbert R.J.C.
2020 - Small Molecule Permeation Across Membrane Channels: Chemical Modification to Quantify Transport Across OmpF
Orbit mini pre-publication in Angewandte Chemie (2020)
Wang J., Bafna J.A., Bhamidimarri S.P., Winterhalter M.
2020 - New Structural insights into Kir channel gating from molecular simulations, HDX-MS and functional studies
Orbit mini publication in Nature Scientific Reports (2020)
Fagnen C., Bannwarth L, Oubella I., Forest E., De Zorzi R., de Araujo A., Mhoumadi Y., Bendahhou S., Perahia D., Vénien-Bryan C.
2020 - Electrophysiology on Channel-Forming Proteins in Artificial Lipid Bilayers
Orbit 16 and Orbit mini Chapter in Patch Clamp Electrophysiology (2020)
Zaitseva E., Obergrussberger A., Weichbrodt C., Boukhet M., Bernhard F., Hein C., Baaken G., Fertig N., Behrends J.C.
2020 - Electrophysiological characterization of transport across outer membrane channels from Gram‐negative bacteria in presence of lipopolysaccharides (LPS)
Port-a-Patch, Vesicle Prep Pro and Orbit mini Publication in Angewandte Chemie International Edition (2020)
Wang J., Terrasse R., Bafna J.A., Benier L., Winterhalter M.
2020 - Cryo-EM structure of human Cx31.3/GJC3 connexin hemichannel
Orbit mini publication in Science Advances (2020)
Authors:
Lee H.J., Jeong H., Hyun J., Ryu B., Park K., Lim H.H., Yoo J., Woo J.S.
2020 - Computational Modeling of Ion Transport in Bulk and through a Nanopore Using the Drude Polarizable Force Field
Orbit mini publication in Journal of Chemical Information and Modeling (2020)
Prajapati J.D., Mele C., Alphan Aksoyoglu M.A., Winterhalter M., Kleinekathöfer U.
2020 - Aerolysin nanopores decode digital information stored in tailored macromolecular analytes
Orbit mini publication in Science Advances (2020)
Cao C., Krapp L.F., Al Ouahabi A., König N.F., Cirauqui N., Radenovic A., Lutz J.F. Dal Peraro M.
2020 - Activation of the archaeal ion channel MthK is exquisitely regulated by temperature
Orbit mini publication in eLife (2020)
Jiang Y., Idikuda V., Chowdhury S., Chanda B.
2019 - Structure of the human ClC-1 chloride channel
Port-a-Patch, Vesicle Prep Pro and Orbit mini publication in PLOS Biology (2019)
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.
2019 - Revisiting an old antibiotic: bacitracin neutralizes binary bacterial toxins and protects cells from intoxication (2)
Orbit mini publication in The FASEB Journal (2019)
Schnell L., Felix I., Müller B., Sadi M., von Bank F., Papatheodorou P., Popoff M.R., Aktories K., Waltenberger E., Benz R., Weichbrodt C., Fauler M., Frick M., Barth H.
2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast
Orbit mini publication in Cells (2019)
Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.
2019 - Arg-8 of yeast subunit e contributes to the stability of F-ATP synthase dimersand to the generation of the full-conductance megachannel
Orbit mini publication in Journal of Biological Chemistry (2019)
Guo L., Carraro M., Carrer A., Minervini G., Urbani A., Masgras I., Tosatto S.C.E., Szabò I., Bernardi P., Lippe G.
2018 - Structures of monomeric and oligomeric forms of the Toxoplasma gondii perforin-like protein 1
Orbit mini and Vesicle Prep Pro publication in Science Advances (2018)
Ni T., Williams S.I., Rezelj S., Anderluh G., Harlos K., Stansfeld P.J., Gilbert R.J.C.
2018 - Functional Analysis of Membrane Proteins Produced by Cell-Free Translation
Port-a-Patch and Orbit mini article in Protein Engineering (2018)
Dondapati S.K., Wüstenhagen D.A., Kubick S.
2018 - Dynamics and number of trans-SNARE complexes determine nascent fusion pore properties
Orbit mini publication in Nature (2018)
Bao H., Das D., Courtney N.A., Jiang Y., Briguglio J.S., Lou X., Roston D., Cui Q., Chanda B., Chapman E.R.
2017 - Molecular determinants of permeation in a fluoride-specific ion channel
Orbit mini in eLife (2017)
Last .B., Sun S., Pham M.C., Miller C.
2017 - Engineering a pH responsive pore forming protein
Orbit mini and Vesicle Prep Pro publication in Scientific Reports (2017)
Kisovec M., Rezelj S., Knap P., Cajnko M.M., Caserman S., Flašker A., Žnidaršič N., Repič M., Mavri J., Ruan Y., Scheuring S., Podobnik M., Anderluh G.
2016 - Mechanistic signs of double-barreled structure in a fluoride ion channel
Orbit mini publication in eLIFE (2016)
Last N.B., Kolmakova-Partensky L., Shane T., Miller C.ポスター
2016 - Next level toxicity screening: From single channel to overall cell behavior
Orbit mini, 
CardioExcyte 96 and 
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, Meeting of the French Society of Toxinology (SFET) 2015 (0.9 MB)
