• Patchliner

    最高の実験柔軟性を誇るオートパッチ
  • Patchliner

    チップの自社内製造と厳格なQC
  • Patchliner

    10年以上のアッセイ系構築/サポート実績
  • Patchliner

    マニュアルパッチの優位性を全て継承
  • Patchliner

    100台以上の導入実績

Neurons - "Stem cell-derived neurons recorded on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.4 MB)
Cells were kindly provided by Cellular Dynamics.

Summary:

Cellular Dynamics International is developing iCell® Neurons, human iPS cell-derived neurons. These neurons have been used on Nanion’s Patchliner, an automated patch clamp device for recording from up to 8 cells simultaneously. Cells were thawed and cultured as per the manufacturer’s instructions. After plating, cells developed neuronal outgrowth within 24 hours and could be kept in culture for 2 weeks. An investigation into voltage- and ligand-gated ion channels expressed in these cells was undertaken using the Patchliner. The first experiments are shown here and offer promising results for combining a cellular model for neurons and an increased throughput patch clamp device.

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