Port-a-Patch mini - Patch more. Pay less
Port-a-Patch miniの登場で, 世界最小のパッチクランプセットがさらに小型化を実現しました。確立したPort-a-Patchの技術を基に，アンプをシステムへ統合することでパッチクランプシステムのさらなる超小型化に成功しています。Port-a-Patch miniは単一細胞のギガシール記録をサポートしており、最小限のトレーニングで高品質なパッチクランプデータに迅速容易に取得できます。強力な研究ツールとしてはもちろん，教育ツールや細胞/イオンチャネルの迅速試験用に最適です。Port-a-Patch mini は以下の特徴と優位性を有しています。
- 超小型，低導入コストの完全なパッチクランプセット
- 細胞および化合物の容易な電気生理的テスト
- パッチクランプアンプを本体に内蔵
- パッチクランプの経験不問
- 教育ツールに最適
- ボルテージ＆カレントクランプ
- ホールセル＆穿孔パッチ
- 社内製造の高品質チップ，豊富なラインナップ（カスタマイズ可能）
- ピペットで化合物を無制限に適用可能
- 細胞株，初代細胞および幹細胞も適用可能
- 簡便，最小限のトレーニングのみ
Port-a-Patch mini はどなたでも簡単にお使い頂けます – ユーザーはディスポ式のパッチクランプチップ上に溶液と細胞懸濁液を適用するだけで，コンピューター制御の陰圧ポンプにより自動的に細胞が捕獲，シール形成されます。
Port-a-Patch mini システムは，アンプ内蔵のPort-a-Patch mini レコーディングユニット，サクションコントロールユニット，コンピューターだけのシンプル構成です。
詳細情報：
PatchControl mini
PatchContol mini: the intuitive Software of the Port-a-Patch mini
The PatchControl mini is a software based on the well-known PatchControl software of the Port-a-Patch and optimized for the Port-a-Patch mini allowing the easiest way to perform your sealing procedure. Equipped with a set of pre-written protocols fitting most user scenarios to obtain a fully automated procedure to rech whole cell configuration, the protocols can also be altered, allowing the entire procedure for cell capture, sealing and obtaining the whole cell configuration to be completely customizable. However, because we know that sometimes you just want to control things yourself, the protocols can be interrupted at any time and the suction controlled manually.
Through innovative communication with the electrophysiology software WinWCP, PatchControl mini can read out important parameters like membrane resistance, whole cell capacitance and series resistance and uses those to perform the pre-programmed automated steps accordingly. Suction strength and duration, voltage and membrane criteria can be set and altered to obtain the optimum parameters for cells routinely used in your lab.
The PatchControl mini software is there to make your patch clamp life easier, from automated cell capture to automated pressure control, the PatchControl mini software can do it all!
専用チップ
NPC-1 パッチクランプチップ
NPC-1 パッチクランプチップは、Port-a-Patch専用に開発された革新的なNanion Technologiesの自社製品です。全てのチップは、厳格なQC管理の下でナニオンのドイツ本社にて製造され、ドイツミュンヘンから世界のユーザーへ発送されます。細胞のサイズやアプリケーションに合わせて、各種のNPC-1チップから最適なチップお選びいただけます。
Material
NPC-1チップは、プレーナー式ホウケイ酸ガラスのスライドにミクロンサイズの穴を穿孔した構造となっています。 このガラス構造を取り扱いが容易なスクリューキャップに組み込むことで、極めて効率的な実験サイクルを実現しています。 さらに、NPC-1チップの独自デザインは細胞外同様に細胞内への容易なアクセスを可能にし、細胞内液の極めて容易な常時灌流を遂に実現しました。チップ 製品ラインナップ
- NPC-1 パッチクランプチップ 1～2 MOhm：(製品コード 061101)
- NPC-1 パッチクランプチップ 2～3.5 MOhm：(製品コード 061102)
- NPC-1 パッチクランプチップ 3～5 MOhm：(製品コード 061103)
- NPC-1 パッチクランプチップ 5～10 MOhm：(製品コード 061104)
- NPC-1 パッチクランプチップ 8～12 MOhm：(製品コード 061105)
- NPC-1 パッチクランプチップ 10～15 MOhm：(製品コード 061106)
特注のホールサイズもご相談いただけます。
上記は製品の基本コードとなります。コード末尾に100, 500, 1000のいずれかを加えて個数指定が必要です。
試薬
Buffers and Solutions for the Port-a-Patch mini
Reliable buffer solutions are critical for any electrophysiological application. Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability. Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. Our buffers are shipped with the corresponding "Certificates of Analysis" and "Material Safety Data Sheets" (MSDS).
Available buffers and solutions
- "External Standard", 500 mL: (Order # 08 3001)
- "External Standard Ca 10", 500 mL: (Order # 08 3012)
- "External NMDG 60", 500 mL: (Order # 08 3004)
- "External NMDG 60 Ca 10", 500 mL: (Order # 08 3011)
- "Internal CsF 110", 500 mL: (Order # 08 3008)
- "Internal KF 110", 500 mL: (Order # 08 3007)
- "Reagent Kit Port-a-Patch Standard", Cryo box with 81 vials, incuding 27x External standard, 18x External Ca 35, 18x Internal KF 60, 18x Internal CsF 60 (Order # 08 1001)
データ＆アプリケーション
Astrocytes - Current Traces of Potassium Channels
Port-a-Patch mini data and applications:
Native mouse Astrocytes.
Patch clamp recordings from cultured mouse primary astrocytes were made in the whole cell configuration. Currents of expressed Potassium channels were obtained by depolarizing voltage pulses from a holding potential of −80 mV.
Chondrocytes - Current response to voltage ramp
Port-a-Patch mini data and applications:
Native Bovine Chondrocytes were kindly provided by Dr. Rebecca Lewis, University of Surrey.
Patch clamp recordings from primary chondrocytes were made in the whole cell configuration. The raw current trace was obtained by using a ramp protocol starting from -100 mV to 100 mV.
hERG - Application of Dofetilide
Port-a-Patch mini data and applications:
Cells were kindly provided by SB Drug Discovery.
Raw data traces of hERG (expressed in HEK cells) in control solution and in the presence of increasing concentrations of Dofetilide (top). IC50 concentration response curve for an average of 3 cells is shown revealing an IC50 of 161 ± 12 nM.
NaV1.5 - Raw current traces and Current-Voltage Relationship
Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.
The image shows currents from an example cell and the corresponding current-voltage plot for NaV1.5 expressed in stably-transfected CHO-K1 cells.
NaV1.5 - Tetracaine concentration response curve
Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.
The image shows the timecourse of the experiment (top) where peak amplitude is plotted against time and example traces in control conditions and different concentrations of tetracaine are also shown in the insert. The concentration reponse curve for tetracaine is shown on the bottom for an average of 6 cells. The curve is fit with a Hill equation revealing an IC50 = 25.5 ± 5.7 µM (n = 6).
NaV1.5-Late - Recorded from Cardiosight-S hiPSC-CMs
Port-a-Patch mini data and applications:
Cells were kindly provided by Nexel.
NaV-Late was induced by application of ATX II in Cardiosight-S® cells on the Port-a-Patch mini. Example traces are shown on the top with the response to the voltage ramp (CiPA protocol) shown in the inset. The timecourse of the experiment is shown on the bottom, with block by ranolazine.
インタビュー&ケーススタディー
Martin Sumser - Statement about the Port-a-Patch mini
"One afternoon is all I needed to get familiar with the features and perform measurements. As an electrophysiologist and university lecturer, I see the Port-a-Patch mini as a great tool to introduce patch clamp to young university students, but also post-doc level scientists in need of a fast, high quality electrophysoplogy data."
Dr. Martin Sumser, Senior Research Scientist
Ludwig-Maximilians University Munich
Bernhard Stenger - Statement about the Port-a-Patch mini
„The new Port-a-Patch mini combines a small and easy set-up with an intuitive software and enabled us to generate data with minimal effort. With the help of a video tutorial we were able to record the effect of AITC on TRPA1 within an hour after setting up the system. This device can be a great tool for testing the effect of compounds on ion channels with the patch clamp technique without spending a lot of time with preparation like priming tubes or pulling pipettes. Especially the possibility of using small volumes (5-15µl) as internal and external solution are an advantage when handling rare and/or toxic substances.”
Dr. Bernhard Stenger, Principal Investigator
Walter-Straub-Institute for Pharmacology and Toxicology, Ludwig-Maximilians University Munich.
ウェビナー＆動画
ダウンロード：
製品カタログ
Port-a-Patch mini - Product Sheet
Port-a-Patch mini product sheet: 
(3.0 MB)
