• Port-a-Patch

    世界最小パッチクランプセットアップ
  • Port-a-Patch

    誰でもデータ取得 - 教育ツールとして最適
  • Port-a-Patch

    細胞, オルガネラ, 脂質二分子膜
  • Port-a-Patch

    世界で最も歴史あるプレーナー式パッチクランプ装置
  • Port-a-Patch

    細胞内灌流実験に最適

Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"

icon pap   Port-a-Patch application note:   logo pdf   (0.5 MB) 

 Summary:

Bacterial membranes are not easy to patch clamp. Since bacterial ion channels are of increasing interest, we started to optimize the protocols for patch clamping bacterial spheroplasts with the Port-a-Patch. Bacterial spheroaplasts can be prepared up to a size of 5 µm. They consist of the inner bacterial membrane. This technique was first used for patch clamp experiments by Boris Matrinac in 1987 and let to the discovery of mechanosensitive channels in E. coli. Here we describe the preparation of Spheroplasts out of E. coli and show ion channel currents recorded with the Port-a-Patch.

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