• Vesicle Prep Pro

    有機溶媒フリーで巨大単層ベシクル(GUV)を自動調製可能な市場初のシステム
  • Vesicle Prep Pro

    均一サイズのリポソームを自動調製 - 多様なアプリケーション

Vesicle Prep Pro - リポソームを簡単に自動調製

Vesicle Prep Pro(ベシクルプレッププロ)は有機溶媒フリーで巨大単層ベシクル(GUVs)を自動調製できる世界初の装置です。GUV は electro-swelling法により作成します。Vesicle Prep Pro は以下の特徴と優位性を有しています:

  • 均一な GUVを作成(直径 1 - 30 μm)
  • 温度コントロール
  • パラメーター設定(温度; 電圧; 周波数)
  • 多様な脂質を使用可能
  • 脂質平面膜実験 / タンパクの活性評価に最適
  • 堅牢、再現性の高い GUV作成
  • ITOスライドは再利用可能

Vesicle Prep Pro は 簡潔なプロトコールでベシクル調製が行えるように設計されており、堅牢かつ再現性の高い調製を可能としています。

ITOコートのスライドガラス上にGUV形成のためのチャンバーがあるため、ベシクルはいつでも顕微鏡で観察可能です。

柔軟なプロトコールデザインと温度コントロールにより、荷電脂質や相転移温度の高い脂質など、多様な脂質からGUVを作成できます。

Vesicle Prep Proで作成したGUVは、起電性の膜貫通タンパク、脂質ラフト形成、タンパク質-DNA相互作用などの生物物理学的特性の研究に有効であることが既に実証されています。

詳細情報:

VesicleControl: ソフトウェア

VesicleControl: Vesicle Prep Pro 専用ソフトウェア

VesicleControl big

VesicleControlソフトウェアは、Vesicle Prep Proで使用する複雑なベシクル作製用プロトコールを作成し、画面で確認できます。電圧、周波数、温度、時間などのパラメータを極めて柔軟に制御し、均一なサイズのベシクル作製できます。また、異なる相転移温度を持つ脂質に対しても、この温度制御により脂質を均一に分布させたベシク作製可能にしています。

試薬

Vesicle Prep Pro 試薬

Vesicle Prep Pro の実験用バッファー、パッチクランプ溶液はナニオン及びパートナー会社で製造後、ドイツのナニオン本社ラボで厳格な品質チェックを行い、ドイツミュンヘンから世界のユーザーへ発送されます。


試薬ラインナップ
  • Vesicle Prep Pro GUVキット: 5 vials x 500 µL GUVs, 13x 100 mM HCl, 9x 1 mL Sorbitol, 1 mM EGTA, 2 mM HEPES (pH 7.0 KOH); 13x 200 mM KCl, 10 mM HEPES (pH 7.0 KOH); 14x 200 mM KCl, 10 mM Hepes (pH 4.0 KOH), 13x 200 mM NaCl, 10 mM Hepes (pH 7.0 NaOH); 14 x 200 mM NaCl, 10 mM Hepes (pH 4.0 NaOH) (製品コード 051003)

インタビュー & ケーススタディー

Dr. Eric Stava - Statement about the Vesicle Prep Pro

icon vpp   “The Vesicle Prep Pro has been of great utility in our Bioelectronics research, allowing students and researchers with limited electrophysiology background to easily form giant unilamellar vesicles (GUVs).  The electronic control lets us alter the size and density of the GUVs, and the transparent chamber allows for excellent visualization of the electro-swelling process.”

Eric Stava, PhD, Research Assistant
University of Wisconsin-Madison, WI, US

Dr. Marisa Rangel - Statement about the Vesicle Prep Pro and Port-a-Patch

icon vpp   icon pap   “Our planar lipid bilayers are obtained from GUVs prepared with the Nanion Vesicle Prep Pro setup. Together with the Port-a-Patch, Nanion´s state-of-the-art automated patch clamp platform, the efficiency of characterization of pore-forming peptides (and other ionophores from natural sources) in artificial bilayers has greatly increased. Both systems are straightforward and easy to use, allowing high quality, low noise, single channel recordings.”

Marisa Rangel, PhD, Researcher 
University of Sao Paulo, Butantan Institute, Sao Paulo, Brazil, WI, US

Prof. Dr. Colin Taylor - Statement about the Port-a-Patch and Vesicle Prep Pro

icon pap   icon vpp   “We are using the Vesicle Prep Pro and Port-a-Patch in our laboratory to study recombinant channel proteins reconstituted in planar bilayers. The system is easy to use and it makes experiments very efficient. Besides the great equipment, Nanion, with their widespread expertise, is also providing excellent continuous support, helping with methodology optimization and problem solving.”

Colin Taylor, Professor of  Cellular Pharmacology, Department of Pharmacology
University of Cambridge, Cambridge, UK

データ & アプリケーション

GUVs - Average Diameter

p44 1 GUVicon vpp   Vesicle Prep Pro data and applications:
The Image was kindly provided of Dr. Sonderman/Prof. Behrends, University of Freiburg, Germany.

The GUVs produced with the Vesicle Prep Pro typically range from 1 – 30 μm in diameter, with an average diameter of
approximately 10 μm (60-70%).

 

Gramicidin - Rapid Internal Solution Exchange

p24 4 InternExchicon pap    Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Switching of internal solutions during gramicidin recordings from a lipid bilayer was obtained within seconds. A lipid bilayer was formed using giant unilamellar vesicles. Currents were recorded at a holding potential of +150 mV. The internal solution was switched from HCl to KCl, resulting in lower channel conductance.

MscL - Pressure Clamp Experiments

p16 2 MscLicon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

The Port-a-Patch and the Suction Control Pro was used to study the effect of pressure on functional MscL protein reconstituted in GUVs. Using symmetrical KCl solutions, MscL gating was observed at -30 mBar and -60 mBar (holding potential + 30 mV), shown as outward currents in the data traces.

Bilayer Recordings - Low Noise

application Low noise holdericon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Typical recording of the Irms noise level of a bilayer (DPhPC) on the Low Capacitance Holder. Typical values are 220 fA

(10 kHz filter frequency, 50 kHz sampling rate. Cf. 2.3 pA RMS on the standard Port-a-Patch).

 

Alpha-Hemolysin - DNA Translocation

p45 4 AlphaHemolysinNeu icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:

Data were kindly supplied by Prof. Fritz Simmel, Technical University of Munich, Munich, Germany.

Reconstituted Alpha hemolysin channels are constantly open at positive and negative membrane potentials. Gating is observed as a result of the passage of a single stranded DNA molecule through the pore. Recordings were performed on the Port-a-Patch.

TRPM8 - Bilayer Recording

TrpM8 420x420icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications: 
Data kindly provided by Dr. Zakharian and Dr. Rohac From UMDNJ New Jersey Medical School, Newark, NJ 07103, USA

Shown are single channel events of TRPM8 reconstituted in a planar lipid bilayer. The recordings were made with the with the Port-a-Patch. The channel was activated by PIP2 and methanol. Currents were recorded at 100 mV.

KcsA - Bilayer Recordings

KCSA Vesicle prep proicon vpp   Vesicle Prep Pro data and applicatons:

The image shows a representative current trace recorded from a planar lipid bilayer in which purified KcsA channels were reconstituted (100 mM KCl, asymmetric pH; pH 4/pH 7). A ramp from -100 mV to +100 mV was used to evoke the single channel events.

Download: Application Note

MscL - Pressure Activation

Vesicle prep pro MscLicon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Boris Martinac, University of QLD, Brisbane, Australia.

Pressure activation of MscL. The MscL channel is gated by pressure, where single channel activity was recorded at 30 mV at the indicated negative hydrostatic pressure. Nanion provides a dedicated suction control device for pressure clamp, found under the Port-a-Patch section.

OmpF - Bilayer Recordings

OMPFPolyamine beaicon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Mathias Winterhalter, Jacobs University, Bremen, Germany.

Channels were activated in the presence of spermine (0.5 mM). The downward spikes represent the closure events due to the effect of spermine. 

Inositol-Triphosphate 3 Receptor (IP3) - Reconstitution

IP3 bea vereinfachticon vpp   Vesicle Prep Pro data and applications:
Data were kindly supplied by Prof. Colin Taylor, University of Cambridge, Cambridge, UK.

Representative traces of the IP3 (inositol-triphosphate) receptor were recorded at different voltages in the presence of IP3 (0.2 mM free Ca2+ and 1 mM Na2ATP, 140 mM KCl). Addition of 2 μM IP3 on the cytosolic side evoked openings of the IP3 receptor.

Cytolysin (Bacterial) - Bilayer Recordings

application bacterial 3icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by ITC & CNR-Istituto di Biofisica, Trento, Italy.

Traces were recorded in 100 mM KCl, 10 mM Hepes, 0.1 mM EDTA, pH 7 at -40 mV. Lipid: Diphtanoyl-PC.

 

Alamethicin - Bilayer Recordings

application alameticin 2icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by M. Sondermann/Prof. Behrends, Univ. Freiburg.

Alamethicin is a channel forming peptide and, when patch clamped, reveals multiple non-equidistant conductance levels due to formation of alamethicin oligomers in the bilayer. Alamethicin single channel conductances. Recordings from a GUV prepared bilayer in 85 mM KCl at -140 mV.

Gramicidin - Single Channel Analysis

application gramicidin 1icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Recordings were kindly supplied by Tohoku University, Tohoku, Japan.

Plotting the current amplitude vs. the voltage reveals conductances of 94.88 pS and 28.28 pS, which correspond to two different gramicidin derivates present in the bilayer. Traces were recorded in 100 mM HCl at the indicated potentials. Clearly two gramicidin derivates (94.88 pS and 28.28 pS) can be distinguished.

Endoplasmatic Reticulum Membrane Protein Translocator (Sec61) - Single Channel recordings

Sec61 Rampicon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro data and applications:
Data were kindly provided by A. Cavalié, M. Jung, R. Zimmermann, Universität des Saarlandes, Homburg, Germany.

Microsomes were fused with GUVs within the VesiclePrepPro. The resulted Proteoliposomes formed a bilayer onto the NPC-1 Chip of the Port-a-Patch. Nice single channel openings could be recorded in symmetrical KCl solution applying a ramp from -110 mV to +100 mV for 400 ms.

ダウンロード:

アプリケーションノート

製品カタログ

Vesicle Prep Pro - Product Sheet

icon vpp   Vesicle Prep Pro product sheet   logo pdf   (2.6 MB)

論文

2018 - Visualizing Tension and Growth in Model Membranes Using Optical Dyes

icon vpp   Vesicle Prep Pro publication in Biophysical Journal (2018)

Authors:
Boyd M.A., Kamat N.P.

2018 - Structures of monomeric and oligomeric forms of the Toxoplasma gondii perforin-like protein 1

Icon Orbit Mini   Orbit mini and   icon vpp   Vesicle Prep Pro publication in Science Advances (2018)

Authors:
Ni T., Williams S.I., Rezelj S., Anderluh G., Harlos K., Stansfeld P.J., Gilbert R.J.C.

2018 - Single channel recording of a mitochondrial calcium uniporter

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in BBRC (2018)

Authors: 
Wu G., Li S., Zong G., Liu, X., Fei S., Shen L., Guan X., Yanga X., Shen Y.

2018 - Romo1 is a mitochondrial nonselective cation channel with viroporin-like characteristics

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Cell Biology (2018)

Authors:
Lee G.Y., You D.G., Lee H.R., Hwang S.W., Lee C.J., Do Yoo Y.

2018 - Regulation of the Pore-Forming Activity of Cecropin A by Local Anesthetics

icon vpp   Vesicle Prep Pro publication in Cell and Tissue Biology (2018)

Authors:
Efimova S.S., Medvedev R.Ya., Chulkov E.G., Schagina L.V., Ostroumova O.S.

2018 - Reconstitution and Electrophysiological Characterization of Ion Channels in Lipid Bilayers

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Current Protocols in Pharmacology (2018)

Authors:
Klaerke D.A., de los Angeles Tejada M., Grøsfjeld Christensen V., Lassen M., Amstrup Pedersen P., Calloe K.

2018 - Probing Interactions between AuNPs/AgNPs and Giant Unilamellar Vesicles (GUVs) Using Hyperspectral Dark-field Microscopy

icon vpp   Vesicle Prep Pro publication in the International Journal of Molecular Sciences (2018)

Authors:
Bhat A., Huan K., Cooks T., Boukari H., Lu Q.

2018 - Phase partitioning, solvent-switchable BODIPY probes for high contrast cellular imaging and FCS

icon vpp   Vesicle Prep Pro publication in New Journal of Chemistry (2018)

Authors:
O’Connor D. Byrne A., Dolana C., Keyes T.E.

2018 - Parallel evolution of frog antimicrobial peptides produces identical conformations but subtly distinct membrane and antibacterial activities

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in bioRxiv (2018)

Authors:
Manzo G., Ferguson P.M., Hind C., Clifford M., Gustilo V.B., Ali H., Bansal S.S., Bui T.T., Drake A.F., Atkinson R.A., Sutton M.J., Lorenz C.D., Phoenix D.A., Mason A.J.

2018 - Minor sequence modifications in temporin B cause drastic changes in antibacterial potency and selectivity by fundamentally altering membrane activity

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in bioRxiv (2018)

Authors:
Manzo G., Ferguson P.M., Gustilo V.B., Ali H., Bui T.T., Drake A.F., Atkinson R.A., Batoni G., Lorenz C.D., Phoenix D.A., Mason A.J.

2018 - Methods for Single-Molecule Sensing and Detection Using Bacteriophage Phi29 DNA Packaging Motor

icon vpp   Vesicle Prep Pro book chapter in Molecular Motors (2018)

Authors:
Haque F, Zhang H, Wang S, Chang C-L, Savran C, Guo P.

2018 - Method for immobilization of living and synthetic cells for high-resolution imaging and single-particle tracking

icon vpp   Vesicle Prep Pro publication in Scientific Reports (2018)

Authors:
Syga Ł., Spakman D., Punter C.M., Poolman B.

2018 - Lipid-mediated regulation of pore-forming activity of syringomycin E by thyroid hormones and xanthene dyes

icon vpp   Vesicle Prep Pro publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2018)

Authors:
Efimova S.S., Zakharova A.A., Ismagilov A.A., Schagina L.V., Malev V.V., Bashkirov P.V., Ostroumova O.S.

2018 - Hybrid vesicles from lipids and block copolymers: Phase behavior from the micro- to the nano-scale

icon vpp   Vesicle Prep Pro publication in Colloids and Surfaces B: Biointerfaces (2018)

Authors:
Magnani C., Montis C., Mangiapia G., Mingotaud A.-F., Mingotaud C., Roux C., Joseph P., Berti D., Lonetti B.

2018 - High contrast imaging and flexible photomanipulation for quantitative in vivo multiphoton imaging with polygon scanning microscope

icon vpp   Vesicle Prep Pro publication in the Journal of Biophotonics (2018)

Authors:
Li Y., Montague S.J., Brüstle A., He X., Gillespie C., Gaus K., Gardiner E.E., Lee W.M.

2018 - Differential interactions of bacterial lipopolysaccharides with lipid membranes: implications for TRPA1-mediated chemosensation

icon vpp   Vesicle Prep Pro publication in Scientific Reports (2018)

Authors:
Startek J.B., Talavera K., Voets T., Alpizar Y.A.

2018 - Development of an on-chip antibiotic permeability assay with single molecule detection capability

icon vpp   Vesicle Prep Pro publication in IEEE Transactions on NanoBioscience (2018)

Authors:
Guzel F.D., Citak F.

2018 - Cholesterol Enriched Archaeosomes as a Molecular System for Studying Interactions of Cholesterol-Dependent Cytolysins with Membranes

icon vpp   Vesicle Prep Pro publication in The Journal of Membrane Biology (2018)

Authors:
Rezelj S., Kozorog M, Švigelj T., Ulrih N.P., Žnidaršič N., Podobnik M., Anderluh G.

2018 - Cargo induces retromer-mediated membrane remodeling on membranes

icon vpp   Vesicle Prep Pro publication in ascb Molecular Biology of the Cell (2018)

Authors:
Purushothaman L.K., Ungermann C.

2018 - An aromatic cluster in Lysinibacillus sphaericus BinB involved in toxicity and proper in-membrane folding

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Archives of Biochemistry and Biophysics (2018)

Authors:
Chooduang A., Surya W., Torres J., Boonserm P.

2018 - Acyl chain asymmetry and polyunsaturation of brain phospholipids facilitate membrane vesiculation without leakage

icon vpp   Vesicle Prep Pro publication in eLife (2018)

Authors:
Manni M.M., Tiberti M.L., Pagnotta S., Barelli H., Gautier R., Antonny B.

2018 - A synthetic enzyme built from DNA flips 10e7 lipids per second in biological membranes

icon vpp   Vesicle Prep Pro publication in the Nature Communications (2018)

Authors:
Ohmann A, Li C-Y, Maffeo C, Al Nahas K, Baumann K.N, Göpfrich K, Yoo J, Keyser U.F, & Aksimentiev A.

2018 - A direct role for hepatitis B virus X protein in inducing mitochondrial membrane permeabilization

icon vpp   Vesicle Prep Pro publication in Journal of Viral Hepatitis (2018)

Authors:
Lee H‐R., Cho Y.Y., Lee G.Y., You D‐g., Yoo Y.D., Kim Y.J.

2017 - The Rotavirus NSP4 Viroporin Domain is a Calcium-conducting Ion Channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature Scientific Reports

Authors:
Pham T., Perry J.L., Dosey T.L., Delcour L.H.,Hyser J.M.

2017 - Polydim-I antimicrobial activity against MDR bacteria and its model membrane interaction

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in PLoS ONE (2017)

Authors: 
Rangel M., Fernandes dos Santos Castro F., Mota-Lima L.D., Clissa P.B., Martins D.B., Perez dos Santos Cabrera M., Mortari M.R.

2017 - Organelle membrane derived patches: reshaping classical methods for new targets

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature Scientific Reports (2017)

Authors:
Shapovalov G., Ritaine A., Bidaux G., Slomianny, C., Borowiec A-S., Gordienko D., Bultynck G., Skryma R., Prevarskaya N.

2017 - Lipid phase separation in the presence of hydrocarbons in giant unilamellar vesicles

icon vpp   Vesicle Prep Pro publication in AIMS Biophysics (2017)

Authors:
Bartelds R., Barnoud J., Boersma A.J., Marrink S.J., Poolman B.

2017 - Ion- and water-binding sites inside an occluded hourglass pore of a trimeric intracellular cation (TRIC) channel

icon vpp   Vesicle Prep Pro publication in BioMed Central Biology (2017)

Authors: 
Ou X., Guo J., Wang L., Yang H., Liu X., Sun J., Liu Z.

2017 - Evaluation of the antimicrobial activity of the mastoparan Polybia-MPII isolated from venom of the social wasp Pseudopolybia vespiceps testacea (Vespidae, Hymenoptera)

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in International Journal of Antimicrobial Agents (2017)

Authors: 
Silva J.C., Neto L.M., Neves R.C., Gonçalves J.C., Trentini M.M., Mucury-Filho R., Smidt K.S., Fensterseifer I.C., Silva O.N., Lima L.D., Clissa P.B., Vilela N., Guilhelmelli F., Silva L.P., Rangel M., Kipnis A., Silva-Pereira I., Franco O.L., Junqueira-Kipnis A.P., Bocca A.L., Mortari M.R.

2017 - Engineering a pH responsive pore forming protein

Icon Orbit Mini  Orbit mini and   icon vpp   Vesicle Prep Pro publication in Scientific Reports (2017)

Authors: 
Kisovec M., Rezelj S., Knap P., Cajnko M.M., Caserman S., Flašker A., Žnidaršič N., Repič M., Mavri J., Ruan Y., Scheuring S., Podobnik M., Anderluh G.

2017 - Bilayer-Mediated Structural Transitions Control Mechanosensitivity of the TREK-2 K2P Channel

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Structure (2017)

Authors: 
Arya P, Jarerattanachat V., Clausen M.V., Schewe M., McClenaghan C., Argent L., Conrad L.J., Dong Y.Y., Pike A.C.W., Carpenter E.P., Baukrowitz T., Sansom M.S.P., Tucker S.J.

2017 - Asymmetric mechanosensitivity in a eukaryotic ion channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in PNAS (2017)

Authors:
Clausen M.V., Jarerattanachata V., Carpenterc E.P., Mark S. , Sansomb P., Tucker S.J.

2016 - The N-terminal Ankyrin Repeat Domain Is Not Required for Electrophile and Heat Activation of the Purified Mosquito TRPA1 Receptor

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature (2016)

Authors: 
Survery S., Moparthi L., Kjellbom P., Högestätt E.D., Zygmunt P.M., Johanson U.

2016 - Structural and functional characterization of a calcium-activated cation channel from Tsukamurella paurometabola

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature Communications (2016)

Authors:
Dhakshnamoorthy B., Rohaim A., Rui H., Blachowicz L., Roux B.

2016 - Pore architecture of TRIC channels and insights into their gating mechanism

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Nature (2016)

Authors: 
Yang H., Hu M., Guo J., Ou X., Cai T., Liu Z.

2016 - Photosensitization in Porphyrias and Photodynamic Therapy Involves TRPA1 and TRPV1

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in The Journal of Neuroscience (2016)

Authors: 
Babes A., Sauer S.K., Moparthi L., Kichko T.I., Neacsu C., Namer B., Filipovic M., Zygmunt P.M., Reeh P.W., Fischer M.J.

2016 - Human TRPA1 is a heat sensor displaying intrinsic U-shaped thermosensitivity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Scientific Reports (2016)

Authors: 
Moparthi L., Kichko T. I., Eberhardt M., Högestätt E. D., Kjellbom P., Johanson U., Reeh P., W., Leffler A., Filipovic M. R., Zygmunt P. M.

2016 - Functional characterization of p7 viroporin from hepatitis C virus produced in a cell-free expression system

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Protein Expression and Purification (2016)

Authors: 
Soranzo T., Cortès S., Gilde F., Kreir M., Picart C., Lenormand J.-L.

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2016 - Conductance and Capacity of Plain Lipid Membranes under Conditions of Variable Gravity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomedical Science and Engineering (2016)

Authors: 
Sieber M., Kaltenbach S., Hanke W. and Kohn F.P.M

2015 - Quantification of Fluoroquinolone Uptake through the Outer Membrane Channel OmpF of Escherichia coli

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2015)

Authors: 
Cama J., Bajaj H., Pagliara S., Maier T., Braun Y., Winterhalter M., Keyser U.F.

2015 - High-Resolution Size-Discrimination of Single Nonionic Synthetic Polymers with a Highly Charged Biological Nanopore

Icon Orbit  Orbit 16 and   icon vpp   Vesicle Prep Pro publication in American Chemical Society Nano (2015)

Authors: 
Baaken G., Halimeh I., Bacri, Pelta J., Oukhaled A., Behrends J.C.

2015 - Different Ligands of the TRPV3 Cation Channel Cause Distinct Conformational Changes As Revealed by Intrinsic Tryptophan Fluorescence Quenching

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)

Authors: 
Billen B., Brams M., Debaveye S., Remeeva A., Alpizar Y.A., Waelkens E., Kreir M., Brüggemann A., Talavera K., Nilius B., Voets T., Ulens C.

2015 - Cell-free expression of a functional pore-only sodium channel

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Protein Expression and Purification (2015)

Authors:
Kovácsová G, Gustavsson E, Wang J, Kreir M, Peuker S, Westenhoff S.

2015 - Bilayer-Spanning DNA Nanopores with Voltage- Switching between Open and Closed State

Icon Orbit  Orbit 16 and   icon vpp   Vesicle Prep Pro publication in American Chemical Society Nano (2015)

Authors: 
Seifert A., Göpfrich K., Burns J.R., Fertig N., Keyser U.F., Howorka S.

2015 - Antibiotic translocation through porins studied in planar lipid bilayers using parallel platforms

Icon Orbit  Orbit 16,   icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Analyst (2015)

Authors:
Weichbrodt C., Bajaj H., Baaken G., Wang J., Guinot S., Kreir M, Behrends J.C., Winterhalter M., Fertig N.

2014 - Temperature-sensitive gating of hCx26: high-resolution Raman spectroscopy sheds light on conformational changes

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biomedical Optics Express (2014)

Authors:
Kniggendorf A.-K., Meinhardt-Wollweber M., Yuan X., Roth B., Seifert A., Fertig N., Zeilinger C.

2014 - Studying mechanosensitive ion channels with an automated patch clamp

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in European Biophysics Journal (2014)

Authors:
Barthmes M.,  Mac Donald F.J., Birkner J.P., Brüggemann A., Wahl-Schott C., Koçer A.

2014 - Structure of a Conserved Golgi Complex-targeting Signal in Coronavirus Envelope Proteins

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biological Chemistry (2014)

Authors: 
Li Y., Surya W., Claudine S., Torres J.

2014 - Membrane assembly of the functional KcsA potassium channel in a vesicle-based eukaryotic cell-free translation system

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biosensors and Bioelectronics (2014)

Authors: 
Dondapati S.K., Kreir M., Quast R.B., Wüstenhagen D.A., Brüggemann A., Fertig N., Kubick S.

2014 - Human TRPA1 is intrinsically cold- and chemosensitive with and without its N-terminal ankyrin repeat domain

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

Authors: 
Moparthi L., Survery S., Kreir M., Simonsen C., Kjellbom P., Högestätt E.D., Johanson U., Zygmunt P.M.

2014 - An outer membrane channel protein of Mycobacterium tuberculosis with exotoxin activity

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2014)

Authors: 
Danilchanka A., Sun J., Pavlenok M., Maueröder C., Speer A., Siroy A., Marrero J., Trujillo C., Mayhew D.L., Doornbos K.S., Muñoz 'L.E., HerrmannM., EhrtS., Berens C., Niederweisa M.

2013 - Lipid-Bilayer-Spanning DNA Nanopores with a Bifunctional Porphyrin Anchor

icon vpp   Vesicle Prep Pro publication in Angewandte Chemie International Edition (2013)

Authors: 
Burns J.R., Göpfrich K., Wood J.W.,Thacker V.V.,Stulz E., Keyser U.F.Howorka S.

2013 - Effect of flavonoids on the phase separation in giant unilamellar vesicles formed from binary lipid mixtures

icon vpp   Vesicle Prep Pro publication in Chemistry and Physics of Lipids (2013)

Authors: 
Ostroumova O.S., Chulkov E.G., Stepanenko O.V., Schagina L.V.

2012 - Natural and artificial ion channels for biosensing platforms

icon pap   Port-a-Patch,   icon pl   Patchliner,   icon sp96   SyncroPatch 96 ((a predecessor model of SyncroPatch 384PE) and   icon vpp   Vesicle Prep Pro publication in Analytical and Bioanalytical Chemistry (2012)

Authors: 
Steller L., Kreir M., Salzer R.

2012 - Hydrophobic gating of mechanosensitive channel of large conductance evidenced by single-subunit resolution

icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States of America (2012)

Authors: 
Birkner JP, Poolman B, Koçer A.

2012 - Green Fluorescent Protein Changes the Conductance of Connexin 43 (Cx43) Hemichannels Reconstituted in Planar Lipid Bilayer

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in The Journal of Biological Chemistry (2012)

Authors: 
Carnarius C., Kreir M., Krick M., Methfessel C., Moehrle V., Valerius O., Brüggemann A., Steinem C., Fertig N.

2012 - Design, synthesis and functional analysis of dansylated polytheonamide mimic: an artificial Peptide ion channel

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of the American Chemical Society (2012)

Authors: 
Itoh H., Matsuoka S., Kreir M., Inoue M.

2011 - Voltage-gated sodium channel (NaV) protein dissection creates a set of functional pore-only proteins

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Proceedings of the National Academy of Sciences of the United States (2011)

Authors: 
Shaya D., Kreir M., Robbins R.A., Wong S., Hammon J., Brüggemann A., Minor Jr. D.L.

2010 - Studying mechanosensitive ion channels using liposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Methods in Molecular Biology (2010)

Authors: 
Martinac B., Rohde P.R., Battle A. R., Petrov E., Pal P, Alexander Foo A.FW, Vásquez V., Huynh T. and Kloda A.

2010 - Permeation of antibiotics through escherichia coli OmpF and OmpC porins: screening for influx on a single-molecule level

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Biomolecular Screening (2010)

Authors: 
Mahendran K.R., Kreir M., Weingart H., Fertig N., Winterhalter M.

2009 - The M34A mutant of Connexin26 reveals active conductance states in pore-suspending membranes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Journal of Structural Biology (2009)

Authors:
Gaßmann O., Kreir M., Ambrosi C., Pranskevich J., Oshima A, Röling C., Sosinsky G., Fertig N., Steinem C.

2009 - Amyloid-beta-Induced Ion Flux in Artificial Lipid Bilayers and Neuronal Cells: Resolving a Controversy

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Neurotoxicity Research (2009)

Authors: 
Capone R., Quiroz F.G., Prangkio P., Saluja I., Sauer A.M., Bautista M.R., Turner R.S., Yang J., Mayer M.

2008 - Rapid screening of membrane protein activity: electrophysiological analysis of OmpF reconstituted in proteoliposomes

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Lab-on-a-chip (2008)

Authors: 
Kreir M., Farre C., Beckler M., George M., Fertig N.

2006 - High-resolution electrophysiology on a chip: Transient dynamics of alamethicin channel formation

icon pap  Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in Biochimica et Biophysica Acta - Biomembranes (2006)

Authors: 
Sondermann M., George M., Fertig N., Behrends J.C.

high phase transition temperatures
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