• Vesicle Prep Pro

    有機溶媒フリーで巨大単層ベシクル(GUV)を自動調製可能な市場初のシステム
  • Vesicle Prep Pro

    均一サイズのリポソームを自動調製 - 多様なアプリケーション

2018 - Single channel recording of a mitochondrial calcium uniporter

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in BBRC (2018)

Authors: 
Wu G., Li S., Zong G., Liu, X., Fei S., Shen L., Guan X., Yanga X., Shen Y.

Journal: 
BBRC (2018) In Press. doi:10.1016/j.bbrc.2018.01.010


Abstract: 

Mitochondrial calcium uniporter (MCU) is the pore-forming subunit of the entire uniporter complex and plays an important role in mitochondrial calcium uptake. However, the single channel recording of MCU remains controversial. Here, we expressed and purified different MCU proteins and then reconstituted them into planar lipid bilayers for single channel recording. We showed that MCU alone from Pyronema omphalodes (pMCU) is active with prominent single channel Ca2+ currents. In sharp contrast, MCU alone from Homo sapiens (hMCU) is inactive. The essential MCU regulator (EMRE) activates hMCU, and therefore, the complex (hMCU-hEMRE) shows prominent single channel Ca2+ currents. These single channel currents are sensitive to the specific MCU inhibitor Ruthenium Red. Our results clearly demonstrate that active MCU can conduct large amounts of calcium into the mitochondria.


Download here

Back

Cookies make it easier for us to provide you with our services. With the usage of our services you permit us to use cookies.
More information Ok