2021 - Alamethicin Channels as a Signal Transduction Element in an Immobilized Single Giant Unilamellar Vesicle
Vesicle Prep Pro publication in Sensors and Materials (2021)
Fukuda H., Nitta M., Sakamoto M., Shoji A., Sugawara M.
Sensors and Materials (2021) doi: 10.18494/SAM.2021.3067
A single giant unilamellar vesicle (GUV) functionalized with an anti-bovine serum albumin (BSA) antibody was immobilized on an avidin slip, and alamethicin channels were embedded as a signal transduction element for creating a channel-based molecular sensing system. The GUV sensor based on the membrane-bound anti-BSA antibody receptor exhibited alamethicin activities that reflected the binding of BSA (an analyte) at the membrane/solution interface. The normalized integrated current at −60 mV was able to be used as a measure of the amount of BSA in a solution. The quantification of BSA at pg/mL level was demonstrated.