201110 blog image EC CIC SURFE2R N1 APP Note

Download the Application Note - CIC from Escherichia coli!

In ClCs are a family of chloride ion channels and transporters with important physiological roles including regulation of the membrane potential, transepithelial salt transport and ion homeostasis. To date, 9 members of the ClC family have been identified in mammals, the first 4 (ClC1, ClC-2, ClC-Ka, and ClC-Kb) are located on the plasma membrane where they act as chloride ion channels whereas the remaining 5 are located in intracellular organelles (ClC-3-7) and are chloride-proton exchangers.

These transporters are important for endosome, lysosome and synaptic vesicle acidification, and mutations in, e.g. ClC-5 underlie the rare chronic kidney disorder, Dent’s Disease, and mutations in ClC-7 underlie osteopetrosis2,4, a rare inherited bone hardening disorder. Given their ubiquitous expression and importance in physiological processes, they are important potential drug targets. ClC from Escherichia coli (Ec-ClC or ClC-ec1) is closelyrelated to its mammalian counterparts and is a Cl-/H+ exchanger. It transports 2 Cl- into the cell, coupled to the efflux of 1 H+. Therefore Ec-ClC is an electrogenic transporter, generating a net charge flow. In E. coli, the Ec-ClC mediates acid resistance of enteric bacteria by promoting H+ extrusion.

Here we present Ec-ClC activity measurements on the SURFE2R N1 instrument using proteoliposomes reconstituted with purified Ec-ClC at different lipid-to-protein ratios.


Data and Applications

Adenine Nucleotide Translocator (ANT) - Recorded on the Surfer

Icon N1   Surfer ANT traceSURFE2R N1 data and applications:

The Adenine Nucleotide Translocator (ANT) is located in the inner membrane of mitochondria and transports, as the name suggests, either ADP or ATP alone or against each other.  Recordings were done on mitochondria inner membrane preparations from pigs heart.  Example traces display the action of ANT either in the presence of ATP, in an other experiment in the presence of ADP.

AmtB - Activation by ammonium

AmtB activation by ammonium

Icon N1 SURFE2R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE2R N1. Shown are transient currents measured after a 100 mM ammonium jump in empty liposomes (green) or proteoliposomes containing AmtB at a lipid protein ratio (LPR) of 50 (black), 10 (red) or 5 (blue). Insert: Normalized current measured in proteoliposomes containing AmtB at an LPR of 50 (black), 10 (red) or 5 (blue).

Data from Mirandela et al, 2018

AmtB - Substrate specificity

AmtB specificity for ammonium

Icon N1 SURFE2R N1 data and applications:

Purified AmtB from E.Coli was incorporated into liposomes and used on the SURFE2R N1. AmtB substrate specificity. (A) Transient current measured after a 100 mM substrate jump. Ammonium (red), methylammonium (black), potassium (green) or sodium (purple). Potassium and sodium do not act as substrates for AmtB and methylammonium translocates but at a much reduced rate compared with ammonium. Insert: Normalised current after a 100 mM substrate jump. Ammonium (red), methylammonium (black).

Data from Mirandela et al, 2018

ChR2 - Cell-based assay optimization

ChR2   Cell based assay optimization Figure6Icon N1   SURFE2R N1 data and applications:

For assay optimization we checked the influence on cell suspension volume we have attached to one single sensor on the signal-to-noise ratio during the SURFE2R experiment.

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ChR2 - Exposure time and frequency of light

ChR2   Exposure time and frequency of light Figure5Icon N1   SURFE2R N1 data and applications:

We investigated the influence of the duration and frequency of the light pulses for ChR2 activation on the stability of the ChR2 signals over time.

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ChR2 - Membrane potential as driving force

ChR2   Membrane potential as driving force Figure4Icon N1   SURFE2R N1 data and applications:

We investigated the effect of a membrane potential - generated by Na+/Ca2+ exchange activity of NCX1 - on the signal amplitude of light induced Na+ flux by ChR2.

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ChR2 - Stability of Retinal binding

ChR2 Stability of Retinal binding Figure1Icon N1   SURFE2R N1 data and applications:

We investigated the effects of retinal addition and removal on light-induced ChR2 activity using a prototypical SURFE2R N1 add-on for activation by light.

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ChR2 - Symmetry of transport

ChR2   Symmetry of transport Figure3Icon N1   SURFE2R N1 data and applications:

Depending on the direction of the Na+ gradient, we observed positive or negative transient currents after ChR2 activation by light. A negative control without ChR2 shows no current signal upon light activation.

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ChR2 - Variation of light intensity

ChR2   Variation of light intensity Figure2Icon N1   SURFE2R N1 data and applications:

We observed saturation kinetics for the light-induced ChR2 activity with increasing LED intensities using a prototypical SURFE2R N1 add-on for activation by light.

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ClC - EC50 for inward and outward directed chloride flux

Clc Figure 2   EC50 Efflux vs InfluxIcon N1   SURFE2R N1 data and applications:

We determined EC50 and relative Vmax for Cl- in an Cl-/H+ exchange assay for both transport directions and found a slight asymmetry in transporter kinetics.

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ClC - Inhibition with OADS

Clc Figure 3   OADS inhibitionIcon N1   SURFE2R N1 data and applications:

We investigated the effect of the specific ClC inhibitor OADS on the transport activity of ClC and its reversibility.



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ClC - Inward and outward directed chloride flux

Clc Figure 1   transient currents Influx vs EffluxIcon N1   SURFE2R N1 data and applications:

According to the transport direction of 2Cl-/1H+ exchange, we observed positive (Cl- efflux) or negative (Cl- influx) transient currents.


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ClC - Proteoliposomes with different lipid-to-protein ratios

Clc Figure 4   LPRsIcon N1   SURFE2R N1 data and applications:

We tested proteoliposomes with different densities of reconstituted ClC protein for their signal-to-noise ratios and compared the obtained signal amplitudes with a negative control: liposomes without reconstituted protein.

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EAAT3 - Current Traces at different Sodium Chloride Concentrations

EAAC1 SURFE2R N1 Website

Icon N1   SURFE2R N1 data and applications:

EAAT3 current traces were recorded after application of different Sodium Chloride concentrations. The EC50 of the peak current was determined as 42.2 mM. 

 

NCX_Mj - EC50 for Calcium and Magnesium

F1 2Icon N1   SURFE2R N1 data and applications:

Measurement of the transport activity of NCX_Mj. The first solution exchange provides a Na+-free solution to establish an outward directed Na+ gradient; the second solution exchange provides the divalent cation to activate the ion exchanger. By applying different concentrations of one divalent cation on the same sensor, we determined EC50 for Ca2+, Mg2+ and Sr2+.

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NCX1 - Block by KB-R7943

NCX1 KBR7943 iCell Cor4U HEK

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytes2, Cor.4U® Cardiomyocytes or HEK cells was blocked by increasing concentrations of KB-R7943.  

 

 

NCX1 - Block by SEA0400

NCX1 iCell HEK SEA0400

Icon N1   SURFE2R N1 data and applications:

Cardiac NCX1 expressed in iCell® Cardiomyocytesor HEK cells was blocked by increasing concentrations of SEA0400, a specific blocker of NCX.  

 

Organic Cation Transporter 2 (OCT2) - Pharmacology

OCT2 SURFE2R N1

Icon N1 SURFE2R N1 data and applications:

EC50 values and relative Vmax for three different substrates are shown.

The IC50 value for Choline was determined as 4.6 mM, for TEA as 0.33 mM and for Metformin as 8.6 mM.

PepT1 - Pharmacology of Lys[Z(NO2)]-Val

Surfer PepT1 traces IC50Icon N1   SURFE2R N1 data and applications:

Representive traces of PepT1 overexpressed in CHO cells. PepT1 (SLC15A1) is expressed mainly in intestine and kidney, it enables uptake of oligopeptides. The PepT1 signal is evoked by glycylglycin. The transport of oligopeptides can be inhibited by addition of Lys[Z(NO2)]-Val. The IC50 of Lys[Z(NO2)]-Val was 380µM.

Respiratory Chain Complex I and III - Current Over Mitochondrial Membrane

Surfer RC complexI III traceIcon N1   SURFE2R N1 data and applications:

In the respiratory chain Complex I and III, oxidation of NADH leads to transport of 4 protons across the membrane. The electrons are transferred to cytochrome c. Because of the electron leakage to oxygen, both complex are the main sites of production of harmful superoxide. In these experiments using the SURFE2R N1, application of 100µM NADH results in a current over the mitochondrial membrane, where Complex I and III are expressed.

Respiratory Chain Complex II and III - Current Traces

Surfer RC II IIIIcon N1   SURFE2R N1 data and applications:

Respiratory Chain Complex II is a succinate dehydrogenase. In this complex, succinate is oxidated to fumarate. The electrons are transfered to the quinone pool, the Complex III (cytochrome bc1 complex) and cytochrome c, while four protons are transported to the intermembrane space.

Respiratory Chain Complex IV - Current Traces

Surfer RC IVIcon N1   SURFE2R N1 data and applications:

The Respiratory Chain Complex IV, known as Cytochrom c oxidase, reduces oxygen by cunsumption of electrons from cytocrome c and transport of two protons to the intermembrans space. Here the reaction runs backwards.

 

 

Respiratory Chain Complex V - Action of the ATP Synthase after Application of ATP

Surfer RC VIcon N1   SURFE2R N1 data and applications:

The Respiratory Chain Complex V uses the transmembrane proton gradient (produced by Complex I, III and IV) to generates ATP from ADP plus phosphate. One component of ATP synthase acts as an ion channel that provides for a proton flux back into the mitochondrial matrix. This reflux releases free energy, which is used to drive ATP synthesis, catalyzed by the other component of the complex. Experiments on the SURFE2R N1 show the action of the ATPase after application of ATP.

SERCA - IC50 for Cisplatin

SERCA Fig 1 partIcon N1   SURFE2R N1 data and applications:

Ca2+-transport in SERCA is triggered by ATP hydrolysis. Using ATP concentration jumps, the authors determined the IC50 of SERCA inhibition by cisplatin

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SGLT1 - Current Traces after Application of alpha-MDG

SGLT1 SURFER N1

Icon N1   SURFE2R N1 data and applications:

Current traces after application of 10 mM and 50 mM alpha-MDG.

 

Sodium-Potassium ATPase - Analysis

Surfer NaK ATPaseIcon N1   SURFE2R N1 data and applications:

The Sodium-Potassium ATPase, also known as Na+/K+ pump is responsible for the active transport of Na+ and K+ in the cells containing relatively high concentrations of K+ ions but low concentrations of Na+ ions. The Na+/K+-ATPase helps maintain resting potential, avail transport, and regulate cellular volume. Here, we demonstrate the conductance of the pump, in the presence of ATP, Na+ and K+, or in the presence of ATP and Na+.

Publications

2021 - Structure and dynamics of the drug-bound bacterial transporter EmrE in lipid bilayers

 Icon N1   SURFE2R N1 publication in Nature Communications(2021)

Authors:
Shcherbakov A.A., Hisao G., Mandala V.S., Thomas N.E., Soltani M., Salter E.A., Davis Jr J.H., Henzler-Wildman K.A., Hong M.

2021 - Structural basis for Potassium transport by KdpFABC

 Icon N1  SURFE2R N1 pre-print in bioRxiv (2021)

Authors:

Sweet M.E., Larsen C., Zhang X., Schlame M., Pedersen B.P., Stokes D.L.

2020 - Unlocking the Reversal Potential of Solid Supported Membrane Electrophysiology to Determine Transport Stoichiometry

Icon N1   SURFE²R N1 pre-publication in bioRxiv (2020)

Authors:
Henzler-Wildman K.A., Thomas N.E.

2020 - The structural basis of promiscuity in small multidrug resistance transporters

 Icon N1   SURFE2R N1 publication in Nature Communications (2020)

Authors:
Kermani A.A., Macdonald C.B., Burata O.E., Koff B.B., Koide A., Denbaum E., Koide S., Stockbridge R.B.

2020 - Protein Adsorption on Solid Supported Membranes: Monitoring the Transport Activity of P-Type ATPases

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review in Molecules (2020)

Authors:
Tadini-Buoninsegni F.

2020 - Label-Free Bioelectrochemical Methods for Evaluation of Anticancer Drug Effects at a Molecular Level

Icon 96SE   SURFE²R 96SE and   Icon N1    SURFE²R N1 Review article in Frontiers in Sensors (2020)

Authors: 
Tadini-Buoninsegni F.,Palchetti I.

2020 - Energy Coupling in Cation-Pumping Pyrophosphatase—Back to Mitchell

Icon N1   SURFE²R N1 publication in Frontiers in Plant Science (2020)

Authors:
Baykov A.A.

2020 - Electrophysiology Measurements of Metal Transport by MntH2 from Enterococcus faecalis

icon sp96 SURFE2R N1 publication in Membranes (2020)

Authors:
Gantner M., Laftsoglou T., Rong H., Postis V.L.G., Jueken L.J.C.

2020 - A two-lane mechanism for selective biological ammonium transport

Icon N1   SURFE²R N1 publication in eLife (2020)

Authors:
Williamson G., Tamburrino G., Bizior A., Boeckstaens M., Mirandela G.D., Bage M.G., Pisliakov A., Ives C.M., Terras E., Hoskisson P.A., Marini A.M., Zachariae U., Javelle A.

2019 - Phosphatidylserine flipping by the P4-ATPase ATP8A2 is electrogenic

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PNAS (2019)

Authors:
Tadini-Buoninsegni F., Mikkelsen S.A., Mogensen L.S., Molday R.S., and Andersen J.P.

2019 - Mutation of two key aspartate residues alters stoichiometry of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

Icon N1   SURFE²R N1 publication in Nature Scientific Reports (2019)

Authors:
Patiño-Ruiz M., Fendler K., & Călinescu O.

2019 - Functional Reconstitution of Membrane Proteins Derived From Eukaryotic Cell-Free Systems

Icon N1   SURFE2R N1 and   Icon Orbit   Orbit 16 publication in Frontiers in Pharmacology (2019)

Authors:
Dondapati S.K., Lübberding H., Zemella A., Thoring L., Wüstenhagen D.A., Kubick S.

2018 - The lipid environment determines the activity of the E. coli ammonium transporter, AmtB

Icon N1   SURFE²R N1 publication in Faseb J. (2018)

Authors:
Mirandela G.D., Tamburrino G., Hoskisson P.A., Zachariae U., Javelle A.

2017 - SSM-Based Electrophysiology for Transporter Research

Icon N1   SURFE²R N1 and   Icon 96SE   SURFE²R 96SE book chapter in Methody in Enzymology

Authors:
Bazzone A., Barthmes M., Fendler K.

2017 - Mechanisms of charge transfer in human copper ATPases ATP7A and ATP7B

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in IUBMB Life (2017)

Authors:
Tadini-Buoninsegni F., Smeazzetto S.

2017 - Insights into the mechanism of membrane pyrophosphatases by combining experiment and computer simulation

Icon N1  SURFE²R N1 publication in Structural Dynamics (2017)

Authors: 
Shah N.R., Wilkinson C., Harborne S.P.D., Turku A., Li K.-M., Sun Y.-J., Harris S., Goldman A.

2017 - Effect of cisplatin on the transport activity of PII-type ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Metallomics (2017)

Authors:
Tadini-Buoninsegni F., Sordi G, Smeazzetto S, Natile G, Arnesano F.

2017 - Discovery of Compounds that Positively Modulate the High Affinity Choline Transporter

Icon N1  SURFE²R N1 publication in Frontiers in Molecular Neuroscience (2017)

Authors: 
Choudhary P., Armstrong E.J., Jorgensen C.C., Piotrowski M., Barthmes M., Torella R., Johnston S.E., Maruyama Y., Janiszewski J.S., Storer R.I., Skerratt S.E., Benn C.L.

2017 - Conformational memory in the association of the transmembrane protein phospholamban with the sarcoplasmic reticulum calcium pump SERCA

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2017)

Authors:
Smeazzetto S., Armanious G.P., Moncelli M.R., Bak J.J., Lemieux M.J., Young H.S., Tadini-Buoninsegni F.

2017 - Competition is the basis of the transport mechanism of the NhaB Na+/H+ exchanger from Klebsiella pneumoniae

Icon N1  SURFE²R-technology (custom-built system)  publication in PLoS ONE (2017)

Authors:
Patiño-Ruiz M., Ganea C., Fendler K., Călinescu O.

2016 - pH Regulation of Electrogenic Sugar/H+ Symport in MFS Sugar Permeases

Icon N1   SURFE²R-technology (custom-built system) publication in PLoS ONE (2016)

Authors:
Bazzone A., Madej M.G., Kaback H.R., Fendler K.

2016 - Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism

Icon N1  SURFE²R N1 publication in Nature Communications (2016)

Authors: 
Li K., Wilkinson C., Kellosalo J., Tsai J., Kajander T, Jeuken L.J.C., Sun Y., Goldman A.

2016 - Functional characterization of solute carrier (SLC) 26/sulfate permease (SulP) proteins in membrane mimetic systems

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2016)

Authors:
Srinivasan L., Baars T.L., Fendler K., Michel H.

2016 - Electrophysiological characterization of the archaeal transporter NCX_Mj using solid supported membrane technology

Icon N1  SURFE²R N1 and   icon vpp   Vesicle Prep Pro publication in Journal of General Physiology (2016)

Authors: 
Barthmes M., Liao J., Jiang Y., Brüggemann A., Wahl-Schott C.

2016 - Electrogenic Cation Binding in the Electroneutral Na+/H+ Antiporter of Pyrococcus abyssi

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2016)

Authors:
Călinescu O., Linder M., Wöhlert D., Yildiz Ö., Kühlbrandt W., Fendler K.

2016 - Charge translocation by mitochondrial NADH:ubiquinone oxidoreductase (complex I) from Yarrowia lipolytica measured on solid-supported membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical and Biophysical Research Communications (2016)

Authors:
Siebels I., Dröse S.

2015 - Structural and Functional Studies of NirC from Salmonella typhimurium

Icon N1   SURFE²R-technology (custom-built system) publication in Methods in Enzymology (2015)

Authors:
Rycovska-Blume A., Lü W., Andrade S., Fendler K., Einsle O.

2015 - Hofmeister effect of anions on calcium translocation by sarcoplasmic reticulum Ca2+-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Nature Scientific Reports (2015)

Authors:
Tadini-Buoninsegni F., Moncelli M.R., Peruzzi N., Ninham B.W., Dei L., Lo Nostroa P.

2015 - A universal mechanism for transport and regulation of CPA sodium proton exchangers

Icon N1  SURFE²R-technology (custom-built system) publication in Biological Chemistry (2015)

Authors:
Călinescu O., Fendler K.

2015 - A sulfur‐based transport pathway in Cu+‐ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in EMBO Reports (2015)

Authors:
Mattle D., Zhang L., Sitsel O., Pedersen L.T., Moncelli M.R., Tadini-Buoninsegni F., Gourdon P., Rees D.C., Nissen P., Meloni G.

2014 - Substrate-bound outward-open state of the betaine transporter BetP provides insights into Na+ coupling

Icon N1  SURFE²R-technology (custom-built system) publication in Nature Communications (2014)

Authors:
Perez C., Faust B., Mehdipour A.R., Francesconi K.A., Forrest L.R., Ziegler C.

2014 - Species differences in bacterial NhaA Na+/H+ exchangers

Icon N1  SURFE²R-technology (custom-built system) publication in FEBS Letters (2014)

Authors:
Călinescu O., Danner E., Böhm M., Hunte C., Fendler K..

2014 - NhaA Na+/H+ antiporter mutants that hardly react to the membrane potential

Icon N1  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Alkoby D., Rimon A., Budak M., Patino-Ruiz M., Călinescu O., Fendler K., Padan E..

2014 - Molecular characterization of the Na+/H+-antiporter NhaA from Salmonella Typhimurium

Icon N1  SURFE²R-technology (custom-built system) publication in PLoS ONE (2014)

Authors:
Lentes C.J., Mir S.H., Boehm M., Ganea C., Fendler K., Hunte C.

2014 - Keeping it simple, transport mechanism and pH regulation in Na+/H+ exchangers

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2014)

Authors:
Călinescu O., Paulino C., Kühlbrandt W., Fendler K.

2014 - Industrializing Electrophysiology: HT Automated Patch Clamp on SyncroPatch 96 Using Instant Frozen Cells

  Icon 96SE  SURFE²R N96 (predecessor model of SURFE²R 96SE) book chapter in Patch-Clamp Methods and Protocols

Authors:
Polonchuk L.

2014 - Direct observation of electrogenic NH4(+) transport in ammonium transport (Amt) proteins

Icon N1   SURFE²R-technology (custom-built system) publication in PNAS (2014)

Authors:
Wacker T., Garcia-Celma J.J., Lewe P., Andrade S.L.

2014 - Anticancer Ruthenium(III) Complex KP1019 Interferes with ATP-Dependent Ca2+ Translocation by Sarco-Endoplasmic Reticulum Ca2+-ATPase (SERCA)

Icon N1  SURFE²R N1 publication in ChemMedChem (2014)

Authors: 
Sadafi F.Z., Massai L., Bartolommei G., Moncelli M.R., Messori L., Tadini-Buoninsegni F.

2013 - Photocycle and vectorial proton transfer in a rhodopsin from the eukaryote Oxyrrhis marina

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2013)

Authors:
Janke C., Scholz F., Becker-Baldus J., Glaubitz C., Wood P.G., Bamberg E., Wachtveitl J., Bamann C.

2013 - Functional characterization of a ClC transporter by solid-supported membrane electrophysiology

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of General Physiology (2013)

Authors:
Garcia-Celma J., Szydelko A., Dutzler R.

2013 - Enhanced adsorption of Ca-ATPase containing vesicles on a negatively charged solid supported membrane for the investigation of membrane transporters

Icon N1   SURFE²R N1 publication in Langmuir (2013)

Authors: 
Sacconi A., Moncelli M.R., Mergheri G., Tadini-Buoninsegni F.

2013 - Electrophysiological characterization of uncoupled mutants of LacY

Icon N1  SURFE²R-technology (custom-built system) publication in Biochemistry (2013)

Authors:
Gaiko O., Bazzone A., Fendler K., Kaback H.R.

2013 - Differential Effects of Mutations on the Transport Properties of the Na+/H+ Antiporter NhaA from Escherichia coli*

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2013)

Authors:
Mager T., Braner M., Kubsch B., Hatahet L., Alkoby D., Rimon A., Padan E., Fendler K.

2012 - The nitrite transport protein NirC from Salmonella typhimurium is a nitrite/proton antiporter

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2012)

Authors:
Rycovska A., Hatahet L., Fendler K., Michel H.

2012 - Investigation of the sodium-binding sites in the sodium-coupled betaine transporter BetP

Icon N1  SURFE²R-technology (custom-built system) publication in PNAS (2012)

Authors:
Khafizov K., Perez C., Koshy C., Quick M., Fendler K., Ziegler C., Forrest L.R.

2012 - Distinctive features of catalytic and transport mechanisms in mammalian sarco-endoplasmic reticulum Ca2+ ATPase (SERCA) and Cu+ (ATP7A/B) ATPases

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2012)

Authors:
Lewis D., Pilankatta R., Inesi G., Bartolommei G., Moncelli M.R., Tadini-Buoninsegni F.

2012 - Assaying the proton transport and regulation of UCP1 using solid supported membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in European Biophysics Journal (2012)

Authors:
Blesneac I., Ravaud S., Machillot P., Zoonens M., Masscheylen S., Miroux B., Vivaudou M., Pebay-Peyroula E.

2011 - Transport Mechanism and pH Regulation of the Na+/H+ Antiporter NhaA from Escherichia coli

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2011)

Authors:
Mager T., Rimon A., Padan E., Fendler K.

2011 - Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

Icon N1  SURFE²R ONE (a predecessor model of the SURFE²R N1) publication in Australian Journal of Chemistry (2011)

Authors:
Gaiko O., Janausch I., Geibel S., Vollert H., Arndt P., Gonski S., Fendler K.

2011 - G117C MelB, a mutant melibiose permease with a changed conformational equilibrium

Icon N1  SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Ganea C., Meyer-Lipp K., Lemonnier R., Krah A., Leblanc G., Fendler K.

2011 - Development of an assay for Complex I/Complex III of the respiratory chain using solid supported membranes and its application in mitochondrial toxicity screening in drug discovery

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2011)

Authors:
Preissl S., Bick I., Obrdlik P., Diekert K., Gul S., Gribbon P.

2011 - Characterisation of the purified human sodium/iodide symporter reveals that the protein is mainly present in a dimeric form and permits the detailed study of a native C-terminal fragment

Icon N1   SURFE²R-technology (custom-built system) publication in Biochimica et Biophysica Acta (BBA) - Biomembranes (2011)

Authors:
Huc-Brandt S., Marcellin D., Graslin F., Averseng O., Bellanger L., Hivin P., Quemeneur E., Basquin C., Navarro V., Pourcher T., Darrouzet E.

2010 - The G215R Mutation in the Cl−/H+-Antiporter ClC-7 Found in ADO II Osteopetrosis Does Not Abolish Function but Causes a Severe Trafficking Defect

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in PLoS ONE (2010)

Authors:
Schulz P., Werner J., Stauber T., Henriksen K., Fendler K.

2010 - Solid-supported membrane technology for the investigation of the influenza A virus M2 channel activity

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Pflügers Archiv - European Journal of Physiology (2010)

Authors:
Balannik V., Obrdlik P., Inayat S., Steensen C., Wang J., Rausch J.M., DeGrado W.F., Kelety B., Pinto L.H.

2010 - Electrophysiology of respiratory chain complexes and the ADP-ATP exchanger in native mitochondrial membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemistry (2010)

Authors:
Watzke N., Diekert K., Obrdlik P.

2010 - Electrophysiological characterization of ATPases in native synaptic vesicles and synaptic plasma membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biochemical Journal (2010)

Authors:
Obrdlik P., Diekert K., Watzke N., Keipert C., Pehl U., Brosch C., Boehm N., Bick I., Ruitenberg M., Volknandt W., Kelety B.

2010 - Delineating electrogenic reactions during lactose/H+ symport

Icon N1  SURFE²R-technology (custom-built system) publication in Biochemistry (2010)

Authors:
Garcia-Celma J.J., Ploch J., Smirnova I., Kaback H.R., Fendler K.

2010 - ATP dependent charge movement in ATP7B Cu+-ATPase is demonstrated by pre-steady state electrical measurements

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2010)

Authors:
Tadini-Buoninsegni F., Bartolommei G., Moncelli M.R., Pilankatta R., Lewis D., Inesi G.

2009 - Measuring Ion Channels on Solid Supported Membranes

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2009)

Authors:
Schulz P., Dueck B., Mourot A., Hatahet L., Fendler K.

2009 - Inhibitory effect of Pb2+ on the transport cycle of the Na+,K+-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Chemical Research in Toxicology (2009)

Authors:
Gramigni E., Tadini-Buoninsegni F., Bartolommei G., Santini G., Chelazzi G., Moncelli M.R.

2009 - Electrophysiological characterization of LacY

Icon N1  SURFE²R-technology (custom-built system) publication in PNAS (2009)

Authors:
Garcia-Celma J.J., Smirnova I.N., Kaback H.R., Fendler K.

2009 - Electrogenic ion pumps investigated on a solid supported membrane: comparison of current and voltage measurements

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Langmuir (2009)

Authors:
Bartolommei G., Moncelli M.R., Rispoli G., Kelety B., Tadini-Buoninsegni F.

2009 - DtpB (YhiP) and DtpA (TppB, YdgR) are prototypical proton-dependent peptide transporters of Escherichia coli

Icon N1  SURFE²R ONE (a predecessor model of SURFE²R N1) publication in The FEBS Journal (2009)

Authors:
Harder D., Stolz J., Casagrande F., Obrdlik P., Weitz D., Fotiadis D., Daniel H.

2009 - An automatic electrophysiological assay for the neuronal glutamate transporter mEAAC1

Icon N1   SURFE²R-technology (custom-built system) publication in Journal of Neuroscience Methods (2009)

Authors:
Krause R., Watzke N., Kelety B., Dörner W., Fendler K.

2008 - Rapid activation of the melibiose permease MelB immobilized on a solid-supported membrane

Icon N1  SURFE²R-technology (custom-built system) publication in Langmuir (2008)

Authors:
Garcia-Celma J.J., Dueck B., Stein M., Schlueter M., Meyer-Lipp K., Leblanc G., Fendler K.

2008 - Identification of the arginine/ornithine antiporter ArcD from Halobacterium salinarum

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in FEBS Letters (2008)

Authors:
Wimmer F., Oberwinkler T., Bisle B., Tittor J., Oesterhelt D.

2008 - Effect of Clotrimazole on the Pump Cycle of the Na,K-ATPase

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Biophysical Journal (2008)

Authors:
Bartolommei G., Devaux N., Tadini-Buoninsegni F., Moncelli M., Apell H.-J.

2007 - Functional and structural characterization of a prokaryotic peptide transporter with features similar to mammalian PEPT1

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biological Chemistry (2007)

Authors:
Weitz D., Harder D., Casagrande F., Fotiadis D., Obrdlik P., Kelety B., Daniel H.

2006 - Transporter assays using solid supported membranes: a novel screening platform for drug discovery

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in ASSAY and Drug Development Technologies (2006)

Authors:
Kelety B., Diekert K., Tobien J., Watzke N., Dörner W., Obrdlik P., Fendler K.

2006 - The inner interhelix loop 4-5 of the melibiose permease from Escherichia coli takes part in conformational changes after sugar binding

Icon N1  SURFE²R-technology (custom-built system) publication in Journal of Biological Chemistry (2006)

Authors:
Meyer-Lipp K., Séry N., Ganea C., Basquin C., Fendler K., Leblanc G.

2006 - Structure and function of prokaryotic glutamate transporters from Escherichia coli and Pyrococcus horikoshii

Icon N1   SURFE²R-technology (custom-built system) publication in Biochemistry (2006)

Authors:
Raunser S., Appel M., Ganea C., Geldmacher-Kaufer U., Fendler K., Kühlbrandt W.

2006 - Establishment of Cell-Free Electrophysiology for Ion Transporters: Application for Pharmacological Profiling

Icon N1   SURFE²R ONE (a predecessor model of SURFE²R N1) publication in Journal of Biomolecular Screening (2006)

Authors:
Geibel S., Flores-Herr N., Licher T., Vollert H.

2005 - Kinetics of charge translocation in the passive downhill uptake mode of the Na+/H+ antiporter NhaA of Escherichia coli

Icon N1   SURFE²R 500 (a predecessor model of SURFE²R N1) publication in Biochimica et Biophysica Acta (BBA) - Bioenergetics (2005)

Authors:
Zuber D., Krause R., Venturi M., Padan E., Bamberg E., Fendler K.

2004 - Time-resolved charge translocation by sarcoplasmic reticulum Ca-ATPase measured on a solid supported membrane

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2004)

Authors:
Tadini Buoninsegni F., Bartolommei G., Moncelli M.R., Inesi G., Guidelli R.

2004 - Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane

Icon N1   SURFE²R-technology (custom-built system) publication in Bioelectrochemistry (2004)

Authors:
Bartolommei G., Buoninsegni F.T., Moncelli M.R.

2003 - Charge displacements during ATP-hydrolysis and synthesis of the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (2003)

Authors:
Burzik C., Kaim G., Dimroth P., Bamberg E., Fendler K.

2002 - Photocurrents Generated by Bacteriorhodopsin Adsorbed on Thiol/Lipid Bilayers Supported by Mercury

Icon N1   SURFE²R-technology (custom-built system) publication in Langmuir (2002)

Authors:
Dolfi A., Tadini-Buoninsegni F., Moncelli M.R., Guidelli R.

2001 - Evidence for intraprotein charge transfer during the transport activity of the melibiose permease from Escherichia coli

Icon N1  SURFE²R-technology (custom-built system) publication in Biochemistry (2001)

Authors:
Ganea C., Pourcher T., Leblanc G., Fendler K.

1999 - Kinetics of electrogenic transport by the ADP/ATP carrier

Icon N1  SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Gropp T., Brustovetsky N., Klingenberg M., Müller V., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na+/K+-ATPase Investigated on Solid Supported Membranes: Cytoplasmic Cation Binding and Release

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K., Bamberg E.

1999 - Charge Translocation by the Na/K-ATPase Investigated on Solid Supported Membranes: Rapid Solution Exchange with a New Technique

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1999)

Authors:
Pintschovius J., Fendler K.

1993 - Charge transport by ion translocating membrane proteins on solid supported membranes

Icon N1   SURFE²R-technology (custom-built system) publication in Biophysical Journal (1993)

Authors:
Seifert K., Fendler K., Bamberg E.

 

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