Automated patch clamp revolutionizes the patch clamp technique making it easier to use and increasing throughput. Automated patch clamp uses a planar borosilicate glass chip rather a fine glass pipette used in conventional patch clamp. The planar glass chip contains a small hole and suction is used from underneath to attract a cell to the hole and form a good seal between the glass and the cell membrane. Electrical currents are measured in the same way in automated and conventional patch clamp and the data quality is comparable between the two techniques. Throughput is increased by recording multiple cells simultaneously and applications such as exchanging the internal recording solution are possible.
Ion channels are integral membrane proteins present in virtually all living cells. They are involved in almost all physiological processes, and their malfunction underlies many disease states, making them important pharmacological targets. Although the conventional patch clamp technique yields information-rich data about ion channels and their biophysical properties, it is low throughput and requires skilled personnel to perform the experiments. Automated patch clamp is much easier to use, and the data generated is comparable to the conventional technique. What is more, multiple cells can be recorded simultaneously, thus increasing throughput for statistical analysis and compound screening.
Whether you are researching channelopathies, screening compound libraries, investigating biophysical properties and stimuli of ion channels, or doing routine cardiac safety screening, Nanion offers the right automated patch clamp instrument for you. With our range of throughput capabilities, unprecedented flexibility, outstanding quality and world-renowned customer support, you can be rest assured that you will make the right choice.
Contact our specialist Dr. Alison Obergrussberger (Scientific Communications Manager). Alison is delighted to help you: