The software for the Patchliner comprises PatchControlHT for controlling the actions of the robot and the experimental workflow, and PatchMaster for electrophysiological data acquisition. PatchControlHT is a graphical user interface for straightforward and easy programming of protocols either by using pre-programmed modules, or by setting up customized protocols based on the specific user requirements. PatchControlHT can run fully automated, controlling the experiments from start to finish, but changes can also be made on-the-fly, a particularly useful feature for assay development and academic research. Because of its inherent logical programming capabilities, experimental redundancy is reduced to a minimum, thus maximizing the data throughput. In addition, PatchControlHT is compatible with various document and database formats which makes compound loading and data analysis faster and easier when screening a large number of compounds.
PatchMaster is a powerful electrophysiology data acquisition software used in manual patch clamp experiments. Voltage protocols from manual patch clamp can be loaded into PatchMaster on the Patchliner and are automatically extended to all 4 or 8 channels at the click of a button. A wide variety of analysis parameters can be chosen in PatchMaster and the online analysis values automatically displayed and exported during the experiment.
Data can easily be analyzed using Nanion's Data Analysis Package, an efficient and convenient data analysis tool. With a few mouse clicks raw data is loaded, displayed, analyzed and pooled, reducing the effort to a minimum. Current-voltage relationship plots and concentration response curves are automatically calculated and displayed. Vhalf values and IC50 or EC50 values are shown for each individual well, and the average values for all active wells are calculated. Raw current traces and time courses are displayed, facilitating judgement of data quality of each recorded well. Individual cells can be easily excluded from the data set and average concentration response curves and IC50 or EC50 values recalculated. Data from several chips and over multiple days can be pooled, and cumulative addition or single point addition regimes can be analyzed.