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SyncroPatch 384

NPC-384 chip

The NPC-384 chip is the cost-efficient and high quality consumable for the SyncroPatch 384. It is produced and quality-assured exclusively in-house at Nanion headquarters and shipped from Munich to our international customers. Different types of NPC-384 chips are available which should be chosen depending on cell size and application to maximize success rates. Recently, we developed dedicated fluoride-free chips (NPC384FF) for high throughput recordings in absence of internal fluoride.

Reference Publication: Rapedius and Obergrussberger et al., Front. Mol. Neurosci. 2022

Description and Material

  • Each NPC-384 chip contains 384 recording chambers
  • 384 well plate with borosilicate glass slide
  • All or part of the chip can be used for experiments, the rest of the chip used at a later timepoint
  • Enables external solution exchange and internal solution perfusion during recording
  • Supports recording of large variety of assays and cell types due to open well format
  • Automated waste removal ensures that the number of exchanges of either the internal or the external solution is unlimited
  • Whole-cell and perforated patch recordings possible
  • Different chip types and aperture sizes to facilitate assay optimization
  • Single-hole or multi-hole chips are available
  • Our new standard chip types are NPC-384T made with thin glass to allow for improved access resistance and voltage control, available in two different aperture sizes (small (S-Type) – and large holes (L-Type))
  • NPC-384FF chips are available for fluoride-free recordings

Open well format

Each well of the 384-well plate of the NPC-384 chip has one (or multiple) apertures for cell capture and recording. The pipette adds solution close to the patch clamp aperture and the solution can be removed from the external side of the well by the pipette. The internal solution is supplied via a perfusion system and solution can be exchanged whilst continuously recording.

Available chip types

NPC-384

  • "NPC-384, 1x medium resistance": one hole per well (Order # 221102)
  • "NPC-384, 1x medium resistance plus": one hole per well (Order # 221104)
  • "NPC-384, 4x medium resistance": four holes per well (Order # 221402)
  • "NPC-384, 1x high resistance": one hole per well (Order # 221101)
  • "NPC-384, 4x high resistance": four holes per well (Order # 221401)
  • "NPC-384, 1x low resistance": one hole per well (Order # 221103)
  • "NPC-384, 4x low resistance": four holes per well (Order # 221403)
  • "NPC-384, 8x": eight holes per well (Order # 221801)

NPC-384T

  • "NPC-384T, 1x S-Type": thin glass, one hole per well (Order # 222101)
  • "NPC-384T, 4x S-Type": thin glass, four holes per well (Order # 222401)
  • "NPC-384T, 8x S-Type": thin glass, eight holes per well (Order # 222801)
  • "NPC-384T, 1x L-Type": thin glass, one hole per well (Order # 222102)

NPC-384FF

  • "NPC-384FF, 1x high resistance": one hole per well (Order # 224101)
  • "NPC-384FF, 1x medium resistance": one hole per well (Order # 224102)
  • "NPC-384FF, 1x low resistance": one hole per well (Order # 224103)
  • "NPC-384FF, 4x high resistance": four holes per well (Order # 224401)
  • "NPC-384FF, 4x medium resistance": four holes per well (Order # 224402)
  • "NPC-384FF, 4x low resistance": four holes per well, (Order # 224403)
  • "NPC-384FF Kit": chips and solutions (Order # 223100)

Buffer solutions

Reliable buffer solutions are critical for any electrophysiological application. Our goal, therefore, is to provide high-class recording solutions that leave you in no doubt about quality and stability. Our quality assurance includes chemical tests as well as tests on our patch clamp systems of each lot. Our buffers are shipped with the corresponding "Certificates of Analysis" and "Material Safety Data Sheets" (MSDS).

How can we help you?

Contact our specialist Dr. Claudia Haarmann (Product Manager of the SyncroPatch 384). Claudia is delighted to help you:

Claudia.Haarmann@nanion.de
or call: +49 89 2190 95-082
or connect via LinkedIn