Assay development
and validation

Accelerating your research with exceptional assays.

If at first you don’t succeed,
try Nanion instead

Finding the right conditions for your assay is important for any successful research program. When you choose to work with Nanion, you will get access to over 30 MSc and PhD level scientists to support experiments, including experienced support for assay development. We have a combined wealth of knowledge about ion channels, biophysics, electrophysiology and cell monitoring and we are happy to share that knowledge with you to ensure you get the most out of your Nanion instruments. We can advise you on cell culture and harvesting protocols as well as buffer solution recommendations and suitable voltage protocols. This saves you time and maximizes your output.

Flexibility of throughput

and consumables

Transferability from lower to higher throughput

For some projects, data from just a few cells may be required, whereas for others a screening a large compound library of hundreds or thousands of compounds may be necessary. You want to be sure that the solutions, cell preparation and voltage protocols are optimal before using precious reagents, cells and consumables. Before embarking on a large compound screen, you can optimize the conditions on the Port-a-Patch or Patchliner and easily transfer the assay to the SyncroPatch 384. In addition, you can use individual wells of the Patchliner and the SyncroPatch 384 in 32-well mode to make your assay development as cost effective as possible.

Find the right consumable

Cells vary in their size and membrane fragility, as well as ion channel expression levels. That's why the NPC chips for the Port-a-Patch, Port-a-Patch mini, Patchliner and SyncroPatch 384 are available with different resistances and as single or multiple holes in each well. Larger patch clamp apertures, i.e. lower resistance are required for cells with a larger size, whereas smaller apertures (higher resistance) are preferable for small or fragile cells. For transiently transfected cells or where current size is small, multiple holes in each well can be beneficial to amplify the current response. Additionally, specialized NPC-384 chips for the SyncroPatch 384 are available for fluoride-free experiments which can be important for some experiments.

NSP-96 plates for the CardioExcyte 96 are available in different sizes and as transparent plates for combining optical readouts with contractility. Sensors for the SURFE2R N1 and MECA 4 recording chips for the Orbit mini are available in different sizes and the MECA 4 Fluo recording chips are compatible with simultaneous fluorescent measurements. The type of consumable is simply chosen at the time of ordering and our specialists are happy to advise you on the right consumable for your experiments.

A wide range of flexible and easy solutions tailored to your challenges

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Assay Development
and validation

Combine assays for a holistic view

Do you want to use multiple techniques to answer your research questions? With Nanion you can combine automated patch clamp with organelle recordings using SSM-based electrophysiology, or find out the effects of mutations at the ion channel level right through to whole cardiomyocyte contractility. Nanion is your one stop shop for all your electrophysiology and cell monitoring needs!

How can we help you?

Contact our specialist Dr. Sonja Stölzle-Feix (Director Scientific Affairs and Product Manager of Cell analytics systems). Sonja is delighted to help you:
or call: +49 89 2190 95-075
or connect via LinkedIn