• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

我们的产品目录

SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16

Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Bacterial Spheroplasts - "Preparation and recordings on the Port-a-Patch of native E. Coli spheroplasts"

icon pap   Port-a-Patch application note:   logo pdf   (0.5 MB) 

 Summary:

Bacterial membranes are not easy to patch clamp. Since bacterial ion channels are of increasing interest, we started to optimize the protocols for patch clamping bacterial spheroplasts with the Port-a-Patch. Bacterial spheroaplasts can be prepared up to a size of 5 µm. They consist of the inner bacterial membrane. This technique was first used for patch clamp experiments by Boris Matrinac in 1987 and let to the discovery of mechanosensitive channels in E. coli. Here we describe the preparation of Spheroplasts out of E. coli and show ion channel currents recorded with the Port-a-Patch.

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