• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

我们的产品目录

SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16

Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Cardiomyocytes - "Recordings of action potentials in mouse ES cell-derived Cor.At cardiomyocytes on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.7 MB)
Cells were kindly provided by Axiogenesis.  

 Summary:

Cor.At® cardiomyocytes display typical cardiac ion channel activity and action potentials. Both the hERG blockers Quinidine and Cisapride, and the Na+-channel blocker Lidocaine, modulated the action potentials. The Quinidine and Lidocaine effects were reversible, the Cisapride effect was non-reversible. The results demonstrate the presence of an array of ion channels in Cor.At® cardiomyocytes which in conjunction are capable of generating action potentials. Hence these stem cell-derived cardiomyocytes are a suitable alternative to primary cardiomyocytes in drug screening and safety testing. In addition, these experiments demonstrate for the first time the suitability of a higher throughput planar patch clamp system, i.e. Nanion´s Patchliner®, for recording action potentials. This is possible because of the flexibility of Nanion‘s patch clamp systems allowing for a multitude of different experiments to be performed in both voltage and current clamp modes. Cells expressing multiple ion channels and therefore able to elicit action potentials, as opposed to cell lines overexpressing a single ion channel subtype, better represent the physiological system. Thus, the Patchliner® in combination with Cor.At® cardiomyocytes form a powerful tool for ion channel research, drug screening and safety testing.

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