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    Nanion技术: 离子通道研究的智能工具

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

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SyncroPatch 384i

SyncroPatch 384i

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Patchliner

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CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

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SURFE²R N1

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Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

KcsA - "Lipid bilayer recordings of KcsA channels reconstituted in proteoliposomes"

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro application note:  logo pdf   (0.2 MB)

Summary:

Solvent-free planar lipid bilayers were formed in an automated manner using suction to attract a giant unilamellar vesicle (GUV) to the patch clamp chip which subsequently bursts across the aperture. Incubation of GUV's with purified KcsA channel protein yielded proteoliposomes. These proteoliposomes allow for immediate recording of channel activity after GUV sealing. The rapid formation of protein-containing planar lipid bilayers is of potential use for the efficient electrophysiological characterization of KcsA as shown here and also other ion channel proteins of interest.

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