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    SyncroPatch 384i: HTS Automated Patch Clamp

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    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

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SyncroPatch 384i

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Vesicle Prep Pro

Vesicle Prep Pro

2018 - Development of an on-chip antibiotic permeability assay with single molecule detection capability

icon vpp   Vesicle Prep Pro publication in IEEE Transactions on NanoBioscience (2018)

Authors:
Guzel F.D., Citak F.

Journal:
IEEE Transactions on NanoBioscience (2018) 99:1-1


Abstract:

Electrophysiology is the method of choice to characterize membrane channels. In this study, we demonstrate a patch pipette based simple miniaturization that allows performing conductance measurements on a planar lipid bilayer in a microfluidic channel. Membrane proteins were reconstituted into Giant Unilamellar Vesicles (GUVs) by electroswelling, and GUVs with a single channel insertion were patched at the tip of pipette. We applied this approach to investigate the interactions of porins from E. coli with single antibiotics, and this will potentially provide information on the permeability rates. The results of this study suggest that this approach can be extended to the integration of several pipettes into the microfluidic channel from different positions, allowing the multiplexed recordings and also reducing the substrate consumption below μL volumes.


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