• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

我们的产品目录

SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16

Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

2018 - Quantifying Permeation of Small Charged Molecules across Channels: Electrophysiology in Small Volumes

icon pap   Port-a-Patch and   icon vpp   Vesicle Prep Pro publication in ACS Omega (2018)

Authors:
Wang J., Benier L., Winterhalter M.

Journal:
ACS Omega (2018) doi: 10.1021/acsomega.8b01611


Abstract:

A major bottleneck in the development of small molecule antibiotics is to achieve good permeability across the outer membrane in Gram-negative bacteria. Optimization with respect to permeability surprisingly lacks appropriate methods. Recently we proposed to use the diffusion potential for charged molecules created by their difference in electrophoretic mobility while crossing the outer membrane channel under a concentration gradient. The latter provides semi-quantitative values but the current available setups require large volumes and thus exclude several classes of molecules. Here we propose a simple approach capturing proteoliposomes at aperture of glass surface (planar aperture or conical glass capillary) decreasing the necessary volume below 50 µL. We measured the transport of two charged molecules sulbactam and ceftazidime across the two major porins in E.coli. Both molecules permeate through these porins were observed with sulbactam owes higher permeability.


Download here

返回总览

Nanion运营博客

Cookies make it easier for us to provide you with our services. With the usage of our services you permit us to use cookies.