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2019 - Protein-free division of giant unilamellar vesicles controlled by enzymatic activity

 icon vpp   Vesicle Prep Pro Pre-publication in BioRxiv (2019)

Authors:
Dreher Y., Spatz J.P., Göpfrich K.

Pre-publication:
BioRxiv (2019) doi: https://doi.org/10.1101/2019.12.30.881557


Abstract:

Cell division is one of the hallmarks of life. Success in the bottom-up assembly of synthetic cells will, no doubt, depend on strategies for the controlled autonomous division of protocellular compartments. Here, we describe the protein-free division of giant unilamellar lipid vesicles (GUVs) based on the combination of two physical principles – phase separation and osmosis. We visualize the division process with confocal fluorescence microscopy and derive a conceptual model based on the vesicle geometry. The model successfully predicts the shape transformations over time as well as the time point of the final pinching of the daughter vesicles. Remarkably, we show that two fundamentally distinct yet highly abundant processes – water evaporation and metabolic activity – can both regulate the autonomous division of GUVs. Our work may hint towards mechanisms that governed the division of protocells and adds to the strategic toolbox of bottom-up synthetic biology with its vision of bringing matter to life.

 


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