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    Nanion技术: 离子通道研究的智能工具

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    SyncroPatch 384i: HTS Automated Patch Clamp

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    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

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    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

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SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

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CardioExcyte 96

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FLEXcyte 96

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Orbit 16

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Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Neurons - "Peri.4U and Dopa.4U stem cell-derived neurons recorded on Nanion´s Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.6 MB)
Cells were kindly provided by Axiogenesis.  

Summary:

Human induced pluripotent stem cell-derived neurons (hiPSC-neurons) may provide a viable cellular model for studying the mechanisms underlying neurological diseases and drug development. Axiogenesis provides a number of hiPSC-neurons including dopaminergic neurons (Dopa.4U) and peripheral neurons (Peri.4U), amongst others. These neurons have been used on in-vitro systems such as multielectrode arrays (MEA), immunocytochemistry and calcium imaging. They are an interesting model for studying neurological diseases such as Parkinson’s Disease, as well as for efficacy, drug discovery and toxicity studies. In this study, the Patchliner was used to record from Dopa.4U and Peri.4U neurons in voltage and current clamp modes. The cell harvesting procedure was optimized (using papain) to ensure that the cells retained proximal dendrites and initial axon segments in order to maintain ion channel expression present in these regions. Due to their irregular shape (presence of processes), success rate (typically 20 - 50% for RSeal >200 MΩ) was lower than other cell types which have a smooth, round shape, e.g. standard cell lines. Voltage-gated Na+ (NaV) and K+ (KV) currents were recorded in both cell types. Action potentials (AP) were also recorded and block of the AP of Dopa.4U cells by lidocaine is shown.

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