• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

我们的产品目录

SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Acetylcholine Receptor Alpha 3 Beta 4 - " Nicotinic a3b4 receptors recorded on Nanion's Patchliner"

icon pl   Patchliner application note:   logo pdf   (0.5 MB)

Summary:

The nicotinic acetylcholine receptor (nAChR) is a member of the ligand-gated ion channel superfamily which includes GABAA, 5HT3, NMDA and glycine receptors. It is a cation-permeable ion channel activated by the neurotransmitter acetylcholine and the natural alkaloid, nicotine. Neuronal nAChR are pentameric and functional channels are formed from a repertoire of nine α (α2 to α10) and three β subunits (β2 to β4). Most nAChR exist as heteromers with the stoichiometry 2α to 3β, however some α subunits function as homomers, these being α7 or α9. nAChR have been proposed to play a role in many neurological disorders such as Alzheimer’s Disease, Parkinson’s, Tourette’s Syndrome and depression. mRNA for the α3 and β4 subunits is found in the mammalian CNS and PNS, in particular automomic ganglion cells and chromaffin cells. nAChR containing the α3 subunit are essential for mediating fast synaptic  transmission in the autonomic nervous system and is essential for survival. Block of nicotinic α3β4 receptors by methadone has also been suggested to play a potential role in analgesia. Here we present data collected on a 4- or 8-channel Patchliner showing the potential use of the Patchliner to record nAChR α3β4 currents activated by nicotine. Nicotine activated α3β4 receptors in a concentration dependent manner with an EC50 similar to those reported in the literature. Nicotinic α3β4 receptors could be repetitively activated by nicotine and blocked by mecamylamine, a ganglionic blocker with clinically relevant hypotensive actions, with an IC50 in good agreement with the literature.

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