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    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

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SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

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Port-a-Patch

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Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

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SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16

Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Glycine receptors (GlyRa1) - "Modulators for glycine receptors investigated using the Patchliner "

icon pl   Patchliner application note:   logo pdf   (0.4 MB)
Cells were kindly provided by AstraZeneca.

 Summary:

The Patchliner was used to study the pharmacology of the hGlyR a1 receptor expressed in mouse fibroblast cell line (L-tk). The Patchliner allows for fast application of drugs (<50 ms) with precisely controlled application intervals and wash times. These are important experimental parameters when investigating ligand gated ion channels and their effectors since many ligand gated ion channels rapidly desensitize. Kinetics and level of desensitization are determined by ligand concentration, exposure time, or both. Because of the Patchliner's rapid solution exchange combined with brief drug exposure, capabilities and timed application intervals, the deleterious effect caused by receptor desensitization can be minimized and corrected for. Here we show the use of Nanion's Patchliner for long (22 s) and short (1 s) application of glycine to patch clamped whole-cells. For the short applications of compound, a stacked solution application protocol was used. Specifically, two zones of solution were aspirated into the pipette used for administration. When applied to the cell, this results in exposure first to the agonist zone followed by a rapid wash out after 1 s of drug application. In all the protocols used, pre-incubation of modulators is possible, as well as washout in the presence of a modulator.

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