• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

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SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

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Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

2015 - Functional Characterization of Prokaryotic NCX by Solid Supported Membrane Technology

Icon N1   SURFE²R N1 poster, Gordon Research Conference 2015  logo pdf   (0.9 MB)

Abstract:

Sodium/calcium exchangers (NCXs) are membrane transporters which play an important role in Ca2+ homeostasis and Ca2+ signaling. These proteins have been implicated in various Ca2+ dependent physiological processes such as neurotransmission, skeletal and smooth muscle contraction, cardiac contractility and apoptosis. Here, we use solid supported membrane (SSM) technology to performed functional analysis of NCX_Mj, an archaeal NCX isoform which has recently been crystalized. Using this approach we characterized the substrate affinity, ion specificity and inhibition by divalent cations of this archaeal exchanger protein. In conclusion we found a high functional similarity of
NCX_Mj and eukaryotic NCX isoforms, although the prokaryotic protein lacks a large regulatory domain. Furthermore our results demonstrate that SSM based technology is well suited for state-of-the-art functional characterization of transporter proteins.

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