• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384i: HTS Automated Patch Clamp

    SyncroPatch 384i: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

我们的产品目录

SyncroPatch 384i

SyncroPatch 384i

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16

Orbit 16

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

2015 - Orbit mini Membrane Preparation Tutorial Video

Icon Orbit Mini   Orbit Mini

Short introduction how to prepare membranes on the Orbit mini

 


Guide:


Manually paint a lipid bilayer on the chip’s four cavities by gently spreading lipid solution over them with e.g. one of the provided tools for painting (brush, Teflon piece in holder, glass rod) 

Nanion recommends to paint bilayers via lipid covered air bubbles:

  1. Take e.g. a 10 μl Eppendorf pipette (small white tip).
  2. Cover the tip with lipids by dipping it into the lipid stock solution without aspirating any of it. Due to capillary forces a small amount of lipid solution will remain in the pipette tip - squeeze out any of these residues from the pipette tip.
  3. Place the tip on the chip’s surface close to a cavity, dispense a small air bubble (2-3 μl) over the cavity and aspirate it back. This results in the formation of a lipid bilayer which can be monitored by following the change in the current response: the signal should not go into saturation anymore and exhibit capacitive properties as shown in detail in the quickstart guide.

Repeat step three on all cavities. If bilayer formation is not successful within a first run start again at step one.

Note: Do not throw the tip away afterwards. It can be still used to repaint broken bilayers during the experiment.

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