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    Nanion技术: 离子通道研究的智能工具

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    SyncroPatch 384i: HTS Automated Patch Clamp

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    SURFE²R 96SE: 非标记高通量转运体筛选

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    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

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SyncroPatch 384

SyncroPatch 384

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CardioExcyte 96

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Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

2004 - Calcium transport by sarcoplasmic reticulum Ca-ATPase can be investigated on a solid-supported membrane

Icon N1   SURFE²R-technology (custom-built system) publication in Bioelectrochemistry (2004)

Authors:
Bartolommei G., Buoninsegni F.T., Moncelli M.R.

Journal:
Bioelectrochemistry (2004) 63(1-2):157-160


Abstract:

Sarcoplasmic reticulum (SR) native vesicles incorporating Ca-ATPase are adsorbed on a solid-supported lipid membrane (SSM). Upon adsorption, the ion pumps are chemically activated by concentration jumps of ATP and the capacitive current transients generated by SR Ca-ATPase are measured under potentiostatic conditions. The Michaelis-Menten constant, KM, for ATP is evaluated by varying the concentration of ATP in the activating solution. This preliminary result shows that ion transport by SR Ca-ATPase can be suitably investigated by a technique based on concentration jumps on an SSM.


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