• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384: HTS Automated Patch Clamp

    SyncroPatch 384: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

我们的产品目录

SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Buffer Solution

Buffer Solution

2022 - Tackling Chronic Compound Responses of hiPSC-CMs for Preclinical Cardiac Risk Evaluation: Defined Serum-Free Medium and Long-Term Culture on the FLEXcyte 96

Icon CE   FLEXcyte 96 poster, BPS 2022  logo pdf   (1.03 MB)

Abstract:

In pre-clinical drug development, cardiac contraction analysis of potential drug candidates is one of the crucial steps to ensure a successful and reliable transition to clinical stages. The use of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) continues to increase in the assessment of safety and toxicological side effects of newly developed compounds, due to their reproducibility and low ethical concern. However, the obstacles of an immature phenotype as well as the need of serum-containing media for chronic assays raise concerns over non-physiological responses in preclinical drug development. To solve this issue, a variety of methods are currently being tested to foster hiPSC-CM maturation in vitro and to conduct chronic studies without the need of serum containing medium. Here we assessed the effects of relatively defined medium (serum-free) and prolonged cell culture times on iCell® Cardiomyocytes² maturation with focus on functional contractile properties using the FLEXcyte 96 technology. Analysis of iCell Cardiomyocytes² contractile properties cultured in iCell Cardiomyocytes Serum-free Medium compared to being cultured in regular serum-containing iCell Cardiomyocytes Maintenance Medium showed similar results for most parameters and expected effects of gold standard compounds assessed over 5 days. In addition, iCell Cardiomyocytes², cultured on FLEXcyte 96 plates and analyzed for 40 days, showed an increase in amplitude and a decrease in beat rate over time, indicating the pro-maturation effect of a physiological environment over time

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