SGLT1 - EC50 for D-glucose transport
SURFE2R N1 data and applications:
Short Description: Na+/sugar translocation is detected using a sugar concentration jump in presence of sodium. Here we determined the KM for glucose transport by SGLT1.
Background and Assay Description: We performed a single solution exchange workflow by applying a D-glucose concentration jump in presence of 300 mM Na+. By application of different D-glucose concentrations in the range between 1 mM and 250 mM on the same sensor, we could determine the EC50 for D-glucose transport by SGLT1. We have used the same concentration of Mannitol as compensation in each substrate-free solution.
Author: Nanion Technologies
Target: SGLT1, SLC5A1
Family/Type: SLC5, Solute:Sodium Symporter Family (SSSF, TCDB: 2.A.21)
Mode of transport: Na+/sugar symport
Sample: Purified membrane vesicles from plasma membrane
Expression: Recombinant overexpression in CHO cells
Platform: SURFE²R N1