• Nanion技术: 离子通道研究的智能工具

    Nanion技术: 离子通道研究的智能工具

  • SyncroPatch 384: HTS Automated Patch Clamp

    SyncroPatch 384: HTS Automated Patch Clamp

  • SURFE²R 96SE: 非标记高通量转运体筛选

    SURFE²R 96SE: 非标记高通量转运体筛选

  • Dynamic Clamp: Patchliner

    Dynamic Clamp: Patchliner

  • 脂双层记录: Orbit产品系列

    脂双层记录: Orbit产品系列

  • CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

    CardioExcyte 96 SOL:用光遗传的手段起搏心肌细胞

我们的产品目录

SyncroPatch 384

SyncroPatch 384

Patchliner

Patchliner

Port-a-Patch

Port-a-Patch

Port-a-Patch mini

Port-a-Patch mini

CardioExcyte 96

CardioExcyte 96

FLEXcyte 96

FLEXcyte 96

SURFE²R 96SE

SURFE²R 96SE

SURFE²R N1

SURFE²R N1

Orbit 16 TC

Orbit 16 TC

Orbit Mini

Orbit Mini

Vesicle Prep Pro

Vesicle Prep Pro

Buffer Solution

Buffer Solution

SGLT1 - EC50 for D-glucose transport

SGLT1 Figure 2   EC50 glucose

Icon N1   SURFE2R N1 data and applications:

Short Description: Na+/sugar translocation is detected using a sugar concentration jump in presence of sodium. Here we determined the KM for glucose transport by SGLT1. 
Read more

 

Background and Assay Description: We performed a single solution exchange workflow by applying a D-glucose concentration jump in presence of 300 mM Na+. By application of different D-glucose concentrations in the range between 1 mM and 250 mM on the same sensor, we could determine the EC50 for D-glucose transport by SGLT1. We have used the same concentration of Mannitol as compensation in each substrate-free solution.

Author: Nanion Technologies
Target: SGLT1, SLC5A1
Family/Type: SLC5, Solute:Sodium Symporter Family (SSSF, TCDB: 2.A.21)
Mode of transport:
Na+/sugar symport
Organism: Human
Sample: Purified membrane vesicles from plasma membrane
Expression: Recombinant overexpression in CHO cells
Platform:   Icon N1   SURFE²R N1

返回总览

We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.