• Aerolysin

    Screenshot s of a typical channel forming aerolysin activity assay with the Orbit 16.

Aerolysin

Family:
Pore-forming Toxins

Description:
Members of this family are proteins/peptides, synthesized by one cell and secreted for insertion into the membrane of another cell where they form transmembrane pores. Pore-forming toxins (PFTs) are the most common bacterial cytotoxic proteins and are required for virulence in a large number of important pathogens.

Subgroups:
Over 100 subgroups belong to the "Pore-forming Toxins" family, including amongst others:
The α-Hemolysin Channel-forming Toxin (αHL) Family
The Aerolysin Channel-forming Toxin (Aerolysin) FamilyThe Botulinum and Tetanus Toxin (BTT) Family
The Pertussis Toxin (PTX) Family
The Crystal Protein (Cry) Family

Aerolysin Background Information


Overview:

The aerolysins are a closely related group of channel-forming toxins secreted by members of the family Aeromonas, important human and animal pathogens. They are activated by host and bacterial proteases which remove a C-terminal fragment of about 40 amino acyl residues. The activated monomeric toxin then binds to a receptor glycosyl phosphatidylinositol (GPI)-anchored protein on the surface of the target cell.


Data Sheet:

Gene:
aerA

Protein
UniProt: P09167 (Aeromonas hydrophila)

Topology:
Homodimer in solution; homoheptamer in the host membrane.

Function:
The mature toxin binds to eukaryotic cells and aggregates to form holes (approximately 3 nm in diameter) leading to the destruction of the membrane permeability barrier and osmotic lysis. Proteolytic cleavage and subsequent release of the propeptide trigger a major conformation change, leading to the formation of a heptameric pre-pore that then inserts into the host membrane.

Assays:
Bilayer Recordings on the Orbit Product family

Reviews and Links

Posters

Publications

2020 - Electrical recognition of the twenty proteinogenic amino acids using an aerolysin nanopore

Icon Orbit  Orbit 16 publication in Nature Biotechnology (2020)

Authors:
Ouldali H., Sarthak K., Ensslen T., Piguet F., Manivet P., Pelta J., Behrends J.C., Aksimentiev A., Oukhaled A.

2019 - A comparison of ion channel current blockades caused by individual poly(ethylene glycol) molecules and polyoxometalate nanoclusters

Icon Orbit   Orbit 16 publication in The European Physical Journal E (2019)

Authors:
Wang H., Kasianowicz J.J., Robertson J.W.F., Poster D.L., Ettedgui J.

2018 - Size-dependent interaction of a 3-arm star poly(ethylene glycol) with two biological nanopores

Icon Orbit   Orbit 16 publication in The European Physical Journal E (2018)

Authors:
Talarimoghari M., Baaken G., Hanselmann R., Behrends J.C.

2016 - Probing driving forces in aerolysin and α-hemolysin biological nanopores: electrophoresis versus electroosmosis

Icon Orbit  Orbit 16 publication in Nanoscale (2016)

Authors:
Boukhet M., Piguet F., Ouldali H., Pastoriza-Gallego M., Pelta J., Oukhaled A.

2015 - High-Resolution Size-Discrimination of Single Nonionic Synthetic Polymers with a Highly Charged Biological Nanopore

Icon Orbit  Orbit 16 and   icon vpp   Vesicle Prep Pro publication in American Chemical Society Nano (2015)

Authors: 
Baaken G., Halimeh I., Bacri, Pelta J., Oukhaled A., Behrends J.C.

 

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