• Aerolysin

    Screenshot s of a typical channel forming aerolysin activity assay with the Orbit 16.

2022 - A Two-Step Calibration Method for Evaluation High Bandwidth Electrochemical Instrument

Icon Orbit  Orbit 16 publication in Journal of Electroanalytical Chemistry (2022)

Authors:
Zhang L.L., Zhong C.B., Li J.G., Niu H.Y., Ying Y.L., Long Y.T.

Journal:
Journal of Electroanalytical Chemistry (2022) doi: 10.1016/j.jelechem.2022.116266


Abstract: 

Single-entity electrochemistry allows the ultrasensitive detection of individual entities by incorporating high bandwidth electrochemical instruments. However, differences among instruments and shielding setups cause large deviations in the recorded signals, which leads to remarkable measurement errors in practical applications. Here, we developed a two-step method to calibrate instruments differences for achieving an accurate single-entity analysis. Two coefficients C1 and C2 were calculated with standard measurement through the model resistors of 1.00 GΩ. C1 obtained from the slope value of the current-voltage (I-V) curve is used to calibrate the shifting of the current statistic distribution. Then, C2, assessed from the standard deviation (STD) noises, is employed to calibrate the full-width half maximum of current statistic distributions. After applying in the model single-molecule experiments of Poly(dA)4 detection with aerolysin nanopores, we showed the effective calibration of measurement differences among four high bandwidth electrochemical instruments. Therefore, this method is generally applicable to all kinds of single-entity electrochemical measurements to reduce measurement errors from instrument differences.


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