DNA Nanopores - Synthetic DNA-based Nanopores
Description:
The design of synthetic membrane channels, constructed entirely from DNA, started only some years ago. The channels, mimicking natural protein channels, are thought to be useful in several different fields, e.g. as biomarkers, in nanofluidics or in drug-delivery processes.
Topology:
A typical DNA membrane channel is assembled from several DNA double helices arranged into a polygon pattern, with the central cavity forming the transmembrane pore. To facilitate insertion of the DNA channel into a lipid bilayer membrane, the DNA helices are chemically modified to carry hydrophobic anchors. Until now, most of the DNA channels featured four or six DNA helices arranged in a square or a hexagon, with the inner channel diameter varying between 1 and 2.5 nm.
Testimonials
Movies
Publications
Orbit 16 and
Orbit Mini publication in Nature Protocols (2021)
Authors:
Lanphere C., Offenbartl-Stiegert D., Dorey A., Pugh G., Georgiou E., Xing Y., Burns J.R., Howorka S.
Vesicle Prep Pro Publication in ACS Appl. Mater. Interfaces (2020)
Authors:
Chen L, Liang S., Chen Y., Wu M., Zhang Y.
Vesicle Prep Pro publication in Nanopore Technology (2020)
Authors:
Göpfrich K., Ohmann A., Keyser U.F.
Orbit Mini publication in ScienceAdvances (2020)
Authors:
Cao C., Krapp L.F., Al Ouahabi A., König N.F., Cirauqui N., Radenovic A., Lutz J.F. Dal Peraro M.
Orbit 16 and
Orbit mini publication in Nature Communications (2019)
Authors:
Diederichs T, Pugh G., Dorey A., Xing, Y., Burns J.R., Nguyen Q.H., Tornow M., Tampé R., & Howorka S
Orbit 16 publication in ACS Nano (2018)
Authors:
Burns J.R., Howorka S.
Vesicle Prep Pro publication in the Nature Communications (2018)
Authors:
Ohmann A, Li C-Y, Maffeo C, Al Nahas K, Baumann K.N, Göpfrich K, Yoo J, Keyser U.F, & Aksimentiev A.
Orbit 16 publication in Nature Communications (2017)
Authors:
Maingi V., Burns J.R., Uusitalo J.J., Howorka S., Marrink S.J., Sansom M.S.P.
Orbit 16 publication in Nature Nanotechnology (2016)
Authors:
Burns J.R., Seifert A., Fertig N, Howorka S.
Orbit 16 and
Vesicle Prep Pro publication in American Chemical Society Nano (2015)
Authors:
Seifert A., Göpfrich K., Burns J.R., Fertig N., Keyser U.F., Howorka S.
Orbit 16 publication in Nano Letters (2013)
Authors:
Burns J.R., Stulz E., Howorka S.
Vesicle Prep Pro publication in Angewandte Chemie International Edition (2013)
Authors:
Burns J.R., Göpfrich K., Wood J.W.,Thacker V.V.,Stulz E., Keyser U.F., Howorka S.