NaV1.5 | sodium voltage-gated channel alpha subunit 5
Family:
Sodium channels
Subgroups:
NaV1.1-1.9
Topology:
Alpha subunits consist of four homologous domains (I-IV) with six transmembrane alpha helices (S1–S6) and a pore-forming loop. One a subunit may associate with 1 or 2 b subunits to make up the channel.
NaV1.5: Background Information
NaV1.5 is found primarily in cardiac muscle, where it mediates the fast influx of Na+-ions (INa) across the cell membrane, resulting in the fast depolarization phase of the cardiac action potential. As such, it plays a major role in impulse propagation through the heart.. NaV1.5 is a TTX-resistent sodium channel.
Gene:
SCN5A
Human Protein:
UniProt Q14524
Tissue:
Cardiac myocytes, uninnervated skeletal muscle, central neurons, gastrointestinal smooth muscle cells and Interstitial cells of Cajal
Function/ Application:
Myocardial conduction, generation of action potentials and cell excitability
Pathology:
Romano-Ward, Brugada syndrome, Jervell, Lange-Nielsen, Long QT syndrome (LQT3), pain, cancer, gastrointestinal: Irritable bowel syndrome
Accessory subunits:
b1, b2, b3, b4
Interaction:
β1, β2, β3, β4 subunit, syntrophin, NEDD4, NEDD4L, WWP2, calmodulin
Modulator:
Aconitine, veratridine, α-scorpion toxin, ATX-II, saxitoxin, tetrodotoxin, lidocaine
Assays:
Patch Clamp: whole cell, room temperature, State- and use-dependence
Particularities:
NaV channel analysis requires GigaOhm seals and a stable and low access resistance
Recommended Reviews:
International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005
Data and Applications
NaV1.5 - Raw current traces and Current-Voltage Relationship
Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.
The image shows currents from an example cell and the corresponding current-voltage plot for NaV1.5 expressed in stably-transfected CHO-K1 cells.
NaV1.5 - Tetracaine concentration response curve
Port-a-Patch mini data and applications:
Cells were kindly provided by Charles River.
The image shows the timecourse of the experiment (top) where peak amplitude is plotted against time and example traces in control conditions and different concentrations of tetracaine are also shown in the insert. The concentration reponse curve for tetracaine is shown on the bottom for an average of 6 cells. The curve is fit with a Hill equation revealing an IC50 = 25.5 ± 5.7 µM (n = 6).
NaV1.5-Late - Recorded from Cardiosight-S hiPSC-CMs
Port-a-Patch mini data and applications:
Cells were kindly provided by Nexel.
NaV-Late was induced by application of ATX II in Cardiosight-S® cells on the Port-a-Patch mini. Example traces are shown on the top with the response to the voltage ramp (CiPA protocol) shown in the inset. The timecourse of the experiment is shown on the bottom, with block by ranolazine.
32- well mode for smaller screens or academic investigations
SyncroPatch 384 data and applications:
Cells were kindly provided by SB Drug Discovery
An exemplary 32-well Mode Experiment. A small fraction of the chip can be used at a time, which is ideal for smaller compound screens.
Consecutive experiments of 32-wells on the same NPC-384 patch clamp chip over multiple days. Success rate and accurate pharmacology remains stable over 8 days as shown in the figure. Nav1.5 recordings in the presence of increasing Mexiletine concentrations.
NaV1.5 - Late Current Analysis using the CiPA Protocol
SyncroPatch 384/768 PE (a predecessor model of the SyncroPatch 384) data and applications:
Cells were kindly provided by Charles River.
Screenshots of the PatchControl 384 software showing NaV1.5 current traces in response to the CiPA voltage step protocol, measured on the SyncroPatch 384PE (a predecessor model of SyncroPatch 384) using whole cell patch clamp methodology and single-hole chips. The NaV1.5 late current was activated by the application of 60 nM ATX-II. The IC50 value of Ranolazine of the late sodium current current was determined as 40.4 µM.
Cardiac Ion Channels - Pharmacology of Sotalol
CardioExcyte 96 and SyncroPatch 384PE (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.
The image on the left hand side displays the results of the blocking effect of Sotalol on hERG. The result is in good agreement with manual patch clamp data (Crumb et al., 2016). The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 10 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility measurements.
Cardiac Ion Channels - Pharmacology of Vandetanib
CardioExcyte 96 and 
Patchliner data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.
The image on the left hand side displays the results of the blocking effect of Vandetanib on hERG, NaV1.5, CaV1.2 and KV4.3. The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 1 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility.
NaV1.5 - Screening Online Analysis
SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:
Cells were kindly provided by Millipore.
The image shows the state dependent block at -130 mV (light blue) and -90 mV (dark blue) by the addition of increasing compound concentrations to the different recording wells. The large window shows the effect of increasing concentrations of Lidocaine (red box) on the hNav1.5 currents.
NaV1.5 - Lidocaine Dose Response
SyncroPatch384PE (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by EMD Millipore
NaV1.5 expressed in HEK293 cells recorded on the SyncroPatch 384PE (a predecessor model of SyncroPatch 384). The concentration response curves for lidocaine block of NaV1.5 were constructed at different holding potential (as indicated) either using a single concentration of compound pera per cell (solid lines) or cumulative concentration response curves (dashed line). The IC50 for lidocaine was shifted by a factor of 35 when holding potential was changed from -120 mV to -80 mV.
NaV1.5 - Inactivation Protocol
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) data and applications:
Cells were kindly provided by EMD Millipore.
Shown are raw current responses of HEK293 cells expressing hNaV1.5 to a double (inactivation) pulse protocol and the corresponding current-voltage plot. The data was fitted with a Boltzmann equation and the Vhalf of inactivation was -84 mV (n = 217).
NaV1.5 - Screening Mode
SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:
Cells were kindly provided by Millipore.
Compound effect on hNav1.5 was investigated. A two-pulse protocol (-90 mV / -130 mV) was used to establish if the different compounds blocked the Nav1.5 currents in a state-dependent manner.
The image shows raw data traces. Success rate for the experiment was 71% (>1 GOhm seal resistance), indicated by the green color.
The corresponding online analysis of this experiment is shown in the data set NaV1.5 - Screening Online Analysis.
NaV1.5 - Stable Access Resistance
Patchliner data and applications:
Cells were kindly provided by Millipore.
The I/V-characteristics of NaV1.5 currents (HEK293) are shown together with the repeated dose dependent block by TTX (lower panel). Five concentrations of TTX (0.3, 1, 3, 10, 30 μM) were applied, followed by washout with antagonist-free buffer and re-application of the same TTX concentrations.
NaV1.5 - Accurate Voltage Control
Port-a-Patch data and applications:
Cells stably transfected with human SCN5A were kindly provided by Millipore.
To perform recordings of fast events, such as the activation and inactivation of Na currents, it is essential to have accurate voltage control. The image shows currents and I/V characteristics of NaV1.5 expressed in HEK293 cells.
NaV1.5 - Current Voltage Relationship
SyncroPatch 384PE (a predecessor model of SyncroPatch 384) data and applications:
Cells were kindly provided by EMD Millipore.
Borosilicate glass chips are used as the patch clamp substrate, ensuring excellent voltage clamp of the cell membrane and high quality seals. Voltage gated channels such as hNaV1.5 (HEK293) have been used to validate the system. This data example shows the current-voltage characteristics and the corresponding raw current traces of a single cell from a recording on the SyncroPatch 384PE (a predecessor model of SyncroPatch 384). The current-voltage plot was fit with a Boltzmann equation revealing a Vhalf of activation of -51 mV for an average of 337 cells.
NaV1.5 - Lidocaine Block
Patchliner data and applications:
Cells were kindly provided by Cytomyx/Millipore.
Full dose response curves at different holding potentials were recorded for each cell (hNav1.5 in HEK293). Currents were elicited by a 10 ms voltage step to 0 mV. Plotted are average peak currents as a function of holding potential and lidocaine concentration.
Application Notes
Cardiac Ion Channels - "High Throughput Screening of Cardiac Ion Channels"
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) Patchliner CardioExcyte 96 application note 
(2.3 MB)
Cardiac Ion Channels - "Simultaneous Assessment of CiPA Stipulated Ion Channels on the SyncroPatch 384PE"
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) application note (1.3 MB)
Cells were kindly provided by Charles River.
Cardiomyocytes - "Combining automated patch clamp, impedance and EFP of hiPSC-CMs"
CardioExcyte 96 SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) Patchliner Application Note
Cells kindly provided by Takara-Clonetech.
Cardiomyocytes - "Impedance and EFP recordings of Pluricyte Cardiomyocytes on the CardioExcyte 96"
CardioExcyte 96 Application Note (1.3 MB)
Cells were kindly provided by Ncardia.
NaV1.5 - "Characterization of CreaCell's hNaV1.5 (A-0822) on Nanion's Patchliner"
Patchliner application note: (0.9 MB)
Cells were kindly provided by CreaCell.
NaV1.5 - "High Throughput Pharmacology of NaV1.5 Channels on Nanion's SyncroPatch 384PE"
SyncroPatch 384i (a predecessor model of the SyncroPatch 384) application note (1.6 MB)
Cells were kindly provided by Millipore.
NaV1.5 - "NaV1.5-ΔKPQ late INa current properties and pharmacology on the SyncroPatch 384i"
SyncroPatch 384i (a predecessor model of the SyncroPatch 384) application note (1.5 MB)
Cells were kindly provided by Metrion Biosciences.
NaV1.5 - "Pharmacology of hNaV1.5 recorded on Nanion's Patchliner"
Patchliner application note: (0.3 MB)
Cells were kindly provided by Millipore.
NaV1.5-Late - "INa-Late recorded from CHO cells and hiPSC-CMs on Nanion´s Patchliner"
Patchliner application note: (0.5 MB)
Cells were kindly provided by Charles River.
iCell® Cardiomyocytes2 were kindly provided by Fujifilm Cellular Dynamics International.
Webinars and Movies
2018 - HTS Phase I study: an update on progress of the CiPA Ion Channel Work Stream using the SyncroPatch 384PE and Patchliner
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i), Patchliner and CardioExcyte 96 Oral Presentation
Presenter:
Tim Strassmaier, Nanion Technologies Inc. USA
Source:
Webinar: "CiPA study: Bridging ion channel and myocyte data", September 12, 2018
03.11.2016 | External Webinar: Accelerating Ion Channel Characterization and New Drug Candidate Identification
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i)
This webinar will show high-throughput functional annotation of human ion channel variants associated with excitation disorders will be described along with use of the Syncropatch 384PE to measure subtype selective activation of KV7 potassium channels as well as inhibition of voltage gated sodium channels like NaV1.7, NaV1.1, and NaV1.5.
Organisation: Icagen Inc.
Presentations
2020 - Validation of a impedance-based phenotypic screening assay able to detect multiple mechanisms of chronic cardiotoxicity in human stem cell-derived cardiomyocytes
CardioExcyte 96 presentation (slide deck) (4.5 MB)
2018 - Innovations for cell monitoring in safety and toxicity assays
CardioExcyte 96 & SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) presentation (slide deck) (3.2 MB)
2017 - HTS in Cardiac Safety
CardioExcyte 96 presentation (slide deck) (4.0 MB)
Posters
2022 - Cardiosafety Testing Based on CiPA - at Scale with SyncroPatch 384 and Genedata Screener
SyncroPatch 384 poster, SLAS 2022 (1.3 MB)
2021 - Reliable identification of cardiac liability in drug discovery using automated patch clamp: Considerations and best practices for high throughput recordings of NaV 1.5
Patchliner and SyncroPatch 384i (a predecessor model of SyncroPatch 384) Physiology 2021 (2 MB)
2018 - Optogenetic technologies enable high throughput ion channel drug discovery and toxicity screening
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) and CardioExcyte 96 poster, Biophysics Annual Meeting 2018 (1.3 MB)
2018 - Combining electrophysiology and contractility recordings for more complete assessment of hiPSC-CMs
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i), Patchliner and 
CardioExcyte 96 poster, Europhysiology Meeting 2018 (1.4 MB)
2017 - Cardiomyocytes in Voltage Clamp and Current Clamp by Automated Patch Clamp
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) and Patchliner poster, BPS Meeting 2017 (1.7 MB)
2015 - High Throughput Automated Patch Clamp of Ion Channels Important in Cardiac Safety and Drug Discovery
SyncroPatch 384PE (a predecessor model of SyncroPatch 384) poster, Chantest Meeting 2015 (1.9 MB)
Publications
2022 - There is no F in APC: Using physiological fluoride-free solutions for high throughput automated patch clamp experiments
SyncroPatch 384 publication in Frontiers in Molecular Neuroscience (2022)
Authors:
Rapedius M., Obergrussberger A., Humphries E.S.A., Scholz S., Rinke-Weiss I., Goetze T.A., Brinkwirth N., Rotordam M.G., Strassmaier T., Randolph A., Friis S., Liutkute A., Seibertz F., Voigt N., Fertig N.
2022 - The G213D variant in Nav1.5 alters sodium current and causes an arrhythmogenic phenotype resulting in a multifocal ectopic Purkinje-related premature contraction phenotype in human-induced pluripotent stem cell-derived cardiomyocytes
CardioExcyte 96 publication in EP Europace (2022)
Authors:
Calloe K., Geryk M., Freude K., Treat J.A., Vold V.A., Frederiksen H.R.S., Broendberg A.K., Frederiksen T.C., Jensen H.K., Cordeiro J.M.
2022 - In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384 instrument) Publication in Nature Communications (2022)
Authors:
Montnach J., Blömer L. A., Lopez L., Filipis F., Meudal H., Lafoux A., Nicolas S., Chu D., Caumes C., Béroud R., Jopling C., Bosmans F., Huchet C., Landon C., Canepari M., De Waard M.
2022 - Genome-wide association analyses identify new Brugada syndrome risk loci and highlight a new mechanism of sodium channel regulation in disease susceptibility
SyncroPatch 768 PE (a predecessor model of the SyncroPatch 384 instrument) publication in Nature Genetics (2022)
Authors:
Barc J., Tadros R., Glinge C., Chiang D.Y., Jouni M., Simonet F., Jurgens S.J., Baudic M., Nicastro M., Potet F., Offerhaus J.A., Walsh R., Choi S.H., Verkerk A.O., Mizusawa Y., Anys S., Minois D., Arnaud M., Duchateau J., Wijeyeratne Y.D., Muir A., Papadakis M., Castelletti S., Torchio M., Ortuño C.G., Lacunza J., Giachino D.F., Cerrato N., Martins R.P., Campuzano O., Van Dooren S., Thollet A., Kyndt F., Mazzanti A., Clémenty N., Bisson A., Corveleyn A., Stallmeyer B., Dittmann S., Saenen J., Noël A., Honarbakhsh S., Rudic B., Marzak H., Rowe M.K., Federspiel C., Le Page S., Placide L., Milhem A., Barajas-Martinez H., Beckmann BM., Krapels I.P., Steinfurt J., Winkel B.G., Jabbari R., Shoemaker M.B., Boukens B.J., Škorić-Milosavljević D., Bikker H., Manevy F.C., Lichtner P., Ribasés M., Meitinger T., Müller-Nurasyid M., KORA-Study Group, Veldink J.H., van den Berg L.H., Van Damme P., Cusi D., Lanzani C., Rigade S., Charpentier E., Baron E., Bonnaud S., Lecointe S., Donnart A., Le Marec H., Chatel S., Karakachoff M., Bézieau S., London B., Tfelt-Hansen J., Roden D., Odening K.E., Cerrone M., Chinitz L.A., Volders P.G., van de Berg M.P., Laurent G., Faivre L., Antzelevitch C., Kääb S., Arnaout A.A., Dupuis JM., Pasquie JL., Billon O., Roberts J.D, Jesel L., Borggrefe M., Lambiase P.D., Mansourati J., Loeys B., Leenhardt A., Guicheney P., Maury P., Schulze-Bahr E., Robyns T., Breckpot J., Babuty D., Priori S.G., Napolitano C., Nantes Referral Center for inherited cardiac arrhythmia, de Asmundis C., Brugada P., Brugada R., Arbelo E., Brugada J., Mabo P., Behar N., Giustetto C., Molina M.S., Gimeno J.R., Hasdemir C., Schwartz P.J., Crotti L., McKeown P.P, Sharma S., Behr E.R., Haissaguerre M., Sacher F., Rooryck C., Tan H.L., Remme C.A., Postema P.G., Delmar M., Ellinor P.T., Lubitz S.A., Gourraud JB., Tanck M.W., George Jr. A.L, MacRae C.A., Burridge P.W., Dina C., Probst V., Wilde A.A., Schott JJ, Redon R., & Bezzina C.R.
2022 - Dominant negative effects of SCN5A missense variants
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384 instrument) Publication in Genetics in Medicine (2022)
Authors:
O'Neill M.J., Muhammad A., Li B., Wada Y., Hall L., Solus J.F., Short L., Roden D.M., Glazer A.M.
2021 - Regulatory effects of non-steroidal anti-inflammatory drugs on cardiac ion channels Nav1.5 and Kv11.1
Port-a-Patch Publication in Chemico-Biological Interactions (2021)
Authors:
Xu Y., Li W., Han Y., Liu H., Zhang S., Yan J., Sun J., Liu Y., Zhang J., Zhao M.
2021 - Induced Pluripotent Stem Cell-Derived Cardiomyocytes with SCN5A R1623Q Mutation Associated with Severe Long QT Syndrome in Fetuses and Neonates Recapitulates Pathophysiological Phenotypes
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384 instrument) Publication in Biology (2021)
Authors:
Hayama E., Furutani Y., Kawaguchi N., Seki A., Nagashima Y., Okita K., Takeuchi D., Matsuoka R., Inai K., Hagiwara N., Nakanishi T.
2021 - Computer modeling of whole-cell voltage-clamp analyses to delineate guidelines for good practice of manual and automated patch-clamp
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384 instrument) Publication in Scientific Reports (2021)
Authors:
Montnach J., Lorenzini M., Lesage A., Simon I., Nicolas S., Moreau E., Marionneau C., Baró I., De Waard M., Loussouar G.
2021 - Antiarrhythmic Hit to Lead Refinement in a Dish Using Patient-Derived iPSC Cardiomyocytes
Patchliner Publication in Journal of Medicinal Chemistry
Authors:
Cashman J.R., Ryan D., McKeithan W.L., Okolotowicz K., Gomez-Galeno J., Johnson M., Sampson K.J., Kass R.S, Pezhouman A, Karagueuzian H.S., Mercola M.,
2020 - Reengineering an Antiarrhythmic Drug Using Patient hiPSC Cardiomyocytes to Improve Therapeutic Potential and Reduce Toxicity
Patchliner and SyncroPatch 768PE (a predecessor model of the SyncroPatch 768i instrument) publication in Cell Stem Cell (2020)
Authors:
McKeithan W. L., Feyen D.A.M., Bruyneel A.A.N., Okolotowicz K.J., Ryan D.A., Sampson K.J., Potet F., Savchenko A., Gómez-Galeno J., Vu M., Serrano R., George Jr. A.L., Kass R.S., Cashman J.R., Mercola M.
2020 - Homocysteine-induced electrical remodeling via the mediation of IP 3 R1/Nav1.5 signaling pathway
Port-a-Patch publication in American Journal of Translational Research (2020)
Authors:
Han L., Wu A., Li Q., Xia Z., Wu Y., Hong K., Xia Z., Li J.
2020 - High-throughput reclassification of SCN5A variants
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) Publication in The American Journal of Human Genetics (2020)
Authors:
Glazer A.M., Wada Y., Li B., Muhammad A., Kalash O.R., O’Neill M.J., Shields T., Hall L., Short L., Blair M.A., Kroncke B.M., Capra J.A., Roden D.M
2020 - GS-967 and Eleclazine Block Sodium Channels in Human Induced Pluripotent Stem Cell-derived Cardiomyocytes
SyncroPatch 768PE (a predecessor model of the SyncroPatch 768i) pre-publication in bioRxiv (2020)
Authors:
Potet F., Egecioglu D.E., Burridge P.W.,George A.L. Jr.
2020 - Disease Phenotypes and Mechanisms of iPSC-Derived Cardiomyocytes From Brugada Syndrome Patients With a Loss-of-Function SCN5A Mutation
Patchliner publication in Frontiers in Cell and Developmental Biology (2020)
Authors:
Li W., Stauske M., Luo X., Wagner S., Vollrath M., Mehnert C.S., Schubert M., Cyganek L., Chen S., Hasheminasab S.M., Wulf G., El-Armouche A., Maier L.S., Hasenfuss G., Guan K.
2020 - Cross-site and cross-platform variability of automated patch clamp assessments of drug effects on human cardiac currents in recombinant cells
Patchliner and SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i instrument) publication in Nature Scientific Reports (2020)
Authors:
Kramer J., Himmel H.M., Lindqvist A., Stoelzle-Feix S., Chaudhary K.W., Li D., Bohme G.A., Bridgland-Taylor M., Hebeisen S., Fan J., Renganathan M., Imredy J., Humphries E.S.A, Brinkwirth N., Strassmaier T., Ohtsuki A., Danker T., Vanoye C., Polonchuk L., Fermini B., Pierson J.B. & Gintant G.
2020 - A general procedure to select calibration drugs for lab-specific validation and calibration of proarrhythmia risk prediction models: An illustrative example using the CiPA model
SyncroPatch 768PE (a predecessor model of the SyncroPatch 768i instrument) publication in the Journal of Pharmacological and Toxicological Methods (2020)
Authors:
Han X., Samieegohar M., Ridder B.J., Wu W.W., Randolph A., Tran P., Sheng J., Stoelzle-Feiz S., Brinkwirth N., Rotordam M.G., Becker N., Friis S., Rapedius M., Goteze T.A., Strassmaier T., Okeyo G., Kramer J., Kuryshev Y., Li Z.
2019 - Electrophysiological evaluation of pentamidine and 17-AAG in human stem cell-derived cardiomyocytes for safety assessment
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) article in European Journal of Pharmacology (2019)
Authors:
Asahi Y., Nomura F., Abe Y., Doi M., Sakakura T., Takasuna K., Yasuda K.
2018 - The opioid oxycodone use‐dependently inhibits the cardiac sodium channel Nav1.5
CardioExcyte 96 publication in British Journal of Pharmacology (2018)
Authors:
Meents J.E., Juhasz K., Stölzle-Feix S., Peuckmann-Post V., Rolke R. Lampert A.
2018 - Multifocal atrial and ventricular premature contractions with an increased risk of dilated cardiomyopathy caused by a Nav1.5 gain-of-function mutation (G213D)
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) -related publication in International Journal of Cardiology (2018)
Authors:
Calloe K., Broendberg A.K., Christensen A.H., Pedersen L.N., Olesen M.S., de los Angeles Tejada M., Friis S., Thomsen M.B., Bundgaard H., Jensen H.K.
2017 - L-Type Calcium Channel Inhibition Contributes to the Proarrhythmic Effects of Aconitine in Human Cardiomyocytes
Patchliner publication in PLoS ONE (2017)
Authors:
Wu J., Wang X., Chung Y.Y. Koh C.H. Liu Z., Guo H., Yuan Q., Wang C., Su S., Wei H.
2017 - Identification of Na+/K+-ATPase inhibition-independent proarrhythmic ionic mechanisms of cardiac glycosides
Patchliner publication in Nature Scientific Reports (2017)
Authors:
Koh C.H., Wu J., Chung Y.Y., Liu Z., Zhang R.R., Chong K., Korzh V., Ting S., Oh S., Shim W., Tian H.Y., Wei H.
2017 - High-throughput electrophysiological assays for voltage gated ion channels using SyncroPatch 768PE
SyncroPatch 768PE (a predecessor model of SyncroPatch 384/768i) publication in PLoS One (2017)
Authors:
Li T, Lu G, Chiang E.Y., Chernov-Rogan T., Grogan J.L., Chen J.
2017 - Correlation between human ether-a-go-go related gene channel inhibition and action potential prolongation
Patchliner publication in British Journal of Pharmacology (2017)
Authors:
Saxena P., Hortigon‐Vinagre M.P., Beyl S.,Baburin I., Andranovits S., Iqbal S.M., Costa A., IJzerman A.P., Kügler P., Timin E., Smith G.L., Hering S.
2017 - A mutant of the Buthus martensii Karsch antitumor-analgesic peptide exhibits reduced inhibition to hNav1.4 and hNav1.5 channels while retaining analgesic activity
Port-a-Patch publication in Journal of Biological Chemistry (2017)
Authors:
Xu Y., Meng X., Hou X., Sun J., Kong X., Sun Y., Liu Z., Ma Y., Niu Y., Song Y., Cui Y., Zhao M., Zhang J.
2016 - Use-dependent Block of Human Cardiac Sodium Channels by GS967
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) publication in Molecular Pharmacology (2016)
Authors:
Potet F., Vanoye C.G., George Jr. A.L.
2016 - Automated Patch Clamp Meets High-Throughput Screening: 384 Cells Recorded in Parallel on a Planar Patch Clamp Module
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) publication in Journal of Lab Automation (2016)
Authors:
Obergrussberger A., Brüggemann A., Goetze T.A., Rapedius M., Haarmann C., Rinke I., Becker N., Oka T., Ohtsuki A., Stengel T., Vogel M., Steindl J., Mueller M., Stiehler J., George M., Fertig N.
2016 - Automated Electrophysiological and Pharmacological Evaluation of Human Pluripotent Stem Cell-Derived Cardiomyocytes
Patchliner publication in Stem Cells and Development (2016)
Authors:
Rajamohan D., Kalra S., Hoang M.D., George V., Staniforth A., Russell H., Yang X., Denning C.
2015 - The Functional Property Changes of Muscular Nav1.4 and Cardiac Nav1.5 Induced by Scorpion Toxin BmK AGP-SYPU1 Mutants Y42F and Y5F
Port-a-Patch publication in Biochemistry (2015)
Authors:
Meng x., Xu Y., Zhao M., Wang F., Ma Y., Jin Y., Liu Y., Song Y., Zhang J.
2015 - Scalable Electrophysiological Investigation of iPS Cell-Derived Cardiomyocytes Obtained by a Lentiviral Purification Strategy
Patchliner publication in Journal of Clinical Medicine (2015)
Authors:
Friedrichs S., Malan D., Voss Y., Sasse P.
2015 - Novel screening techniques for ion channel targeting drugs
Patchliner, SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and CardioExcyte 96 publication in Channels (2015)
Authors:
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.
2014 - New strategies in ion channel screening for drug discovery: are there ways to improve its productivity?
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) publication in Journal of Laboratory Automation (2014)
Authors:
Farre C., Fertig N.
2012 - Isolation, characterization and total regioselective synthesis of the novel μO-conotoxin MfVIA from Conus magnificus that targets voltage-gated sodium channels
Port-a-Patch publication in Biochemical Pharmacology (2012)
Authors:
Vetter I., Dekan Z., Knapp O., Adams D.J., Alewood P.F., Lewis R.J.
2011 - State-of-the-art automated patch clamp devices: heat activation, action potentials, and high throughput in ion channel screening
Port-a-Patch, Patchliner and SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) publication in Frontiers in Pharmacology (2011)
Authors:
Stoelzle S., Obergrussberger A., Brüggemann A., Haarmann C., George M., Kettenhofen R., Fertig N.
2010 - Propranolol blocks cardiac and neuronal voltage-gated sodium channels
Patchliner publication in Frontiers in Pharmacology (2010)
Authors:
Wang D.W., Mistry A.M., Kahlig K.M., Kearney J.A, Xiang J., George Jr. A.L.
2009 - Port-a-Patch and Patchliner: High fidelity electrophysiology for secondary screening and safety pharmacology
Port-a-Patch and Patchliner publication in Combinatorial Chemistry & High Throughput Screening (2009)
Authors:
Farre C., Haythornthwaite A., Haarmann C., Stoelzle S., Kreir M., George M., Brüggemann A., Fertig N.
2007 - Automated ion channel screening: patch clamping made easy
Port-a-Patch and Patchliner publication in Expert Opinion Therapeutic Targets (2007)
Authors:
Farre C., Stoelzle S., Haarman C., George M., Brueggemann A., Fertig N.
2006 - Microchip technology for automated and parallel patch clamp recording
Port-a-Patch and Patchliner publication in Small Journal (2006)
Authors:
Brüggemann A., Stoelzle S., George M., Behrends J.C., Fertig N.