• nAChRα3β4

    repetitive activation by nicotine measured on the

  • nAChRα3β4

    nicotine concentration response curve
    measured on the Patchliner

  • nAChRα3β4

    block by mecamylamine
    recorded on the Patchliner

AChRa3b4 | nicotinic acetylcholine receptor alpha 3 beta 4

Acetylcholine Receptors

The family has been devided into nicotinic acetylcholine receptors (ionotropic channels) and muscarinic acetylcholine receptors (metabotropic G-protein coupled receptors). Both subgroups are sensitive to acetylcholine.

Nicotinic acetylcholine receptors: As typical cys-loop receptors, acetylcholine receptor subunits contain four transmembrane spanning domains TM1-TM4. Five subunits form a pore, homomeric and heteromeric combinations are possible.17 vertebrate subunits have been identified: α genes: CHRNA1 - CHRNA10; β genes: CHRNB1 . CHRNB4, as well as CHRND (delta), CHRNE (epsilon), CHRNG (gamma)
Muscarinic acetylcholine receptors: 5 receptors have been described (M1 - M5), encoded by the 5 genes CHRM1 - CHRM5. These proteins contain seven transmembrane regions and do not form homo or heteromers. 

AChRα3β4: Background Information


Human Protein:
UniProt P32297, P30926

Brain, retina, spinal cord, peripheral neuronal tissues, including neurons of mammalian sympathetic ganglia, parasympathetic ganglia, sensory neurons such as the trigeminal ganglia and adrenal chromaffin cells

Function/ Application:
Mediates effects of acetylcholine and nicotine in specific regions of the brain, retina and spinal cord

Alzheimer's disease, Parkinson's, Tourette's Syndrome, depression

Accessory subunits:


DHβE, mecamylamine, α-conotoxin, hexamethonium, A-867744, NS1738

Patch Clamp: whole cell, room temperature

Cell systems:
Stably-transfected HEK293 cell line

Recommended Reviews:
Physiol Rev 89: 73–120, 2009; doi:10.1152/physrev.00015.2008, Albuquergue et al; Nicotinic acetylcholine receptors, in: Burger’s Medicinal Chemistry, Vol II Drug Discovery and Drug Development, Chapter 11, pp 357-405. Ed. Abraham, D., J. Wiley, New York., Colquhoun et al.

Data and Applications

Acetylcholine Receptor Alpha 3 Beta 4 - Activation


icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications:

Shown is a raw current response of a HEK293 cell expressing AChR (α3β4) to exposure to 300 μM nicotine. Solutions were  stacked (layered) in the pipette to achieve brief exposure times.


Acetylcholine Receptor Alpha 3 Beta 4 - Concentration Response Curve to Nicotine


icon pl  Patchliner data and applications:

Shown are the a raw current responses of a HEK293 cell expressing AChR (α3β4) to increasing concentrations of nicotine. Solutions were  stacked (layered) in the pipette to achieve brief exposure times.


Application Notes


2020 - Discovery and Development of SUVN-911: A Novel, Potent, Selective, and Orally Active Neuronal Nicotinic Acetylcholine α4β2 Receptor Antagonist for the Treatment of Depression

icon pl  Patchliner publication in Journal of Medicinal Chemistry (2020)

Nirogi R., Mohammed A.R., Shinde A.K., Ravella S.R., Bogaraju N., Subramanian R., Mekala V.R., Palacharla R.C., Muddana N., Thentu J.B., Bhyrapuneni G., Abraham R., Jasti V.



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