P2X2/ P2X3 - Application of ATP on the Patchliner

P2X23 1321N1Cells ATP PLicon pl   Patchliner data and applications: 
Cells were kindly supplied by Evotec AG, Hamburg, Germany

P2X2/3 recorded from 1321N1 cells on the Patchliner. P2X2/3 was activated by increasing concentrations of ATP with an EC50 = 7.8 ± 1.0 µM (n = 10). ATP was applied for approximately 600 ms using the stacked solutions function of the Patchliner (holding potential = -80mV).

 

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