Ryanodine Receptors | RyRs
Family:
Ryanodine receptor family
Topology and Members:
Three members are known in human:
RyR1: Is primarily expressed in skeletal muscle
RyR2: Is primarily expressed in myocardium
RyR3: Is expressed more widely, but especially in the brain
The channels contain six transmembrane helices, are composed of mostly homotetramers (heterotetramers are as well described) and complex with further proteins which modulate the channel functions.
Function:
Ryanodine receptors form a class of intracellular calcium channels in various forms of excitable animal tissue. Located in the sarcoplasmic/ endoplasmic reticulum membrane, the channels mediate the release of calcium ions from intracellular stores into the cytoplasm.
Data and Applications
Ryanodine Receptor - Application of Na-ATP and Ryanodine
Orbit 16 and applications:
Data were kindly provided by Ionera.
Traces illustrating RYR single channel activity in the planar lipid bilayer recorded on the Orbit 16.
The RYR channel was reconstituted via fusion of sarcoplasmic reticulum vesicles with preformed asolectin bilayer.
(A) Activity after vesicle fusion and buffer exchange on cis-side. (B) 120 s after addition of 1 mM Na-ATP to the cis-side. (C) 120 s after addition of 5 µM Ryanodine to the cis-side.
Conditions: Trans-side: 53 mM Ba(OH)2, 1 mM Ca2+; Cis-side: 150 nM Ca2+, VHold: 0 mV in all cases
Publications
2021 - Pathological conformations of disease mutant Ryanodine Receptors revealed by cryo-EM
Orbit mini publication in Nature Communications (2021)
Authors:
Woll K.A., Haji-Ghassemi O., Van Petegem F.
2018 - Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
CardioExcyte 96 publication in Stem Cells and Development
Authors:
Kondrashov A., Hoang M.D., Smith J., Bhagwan J., Duncan G., Mosqueira D., Munoz M., Vo N.T.N., Denning C.
2017 - Organelle membrane derived patches: reshaping classical methods for new targets
Port-a-Patch and 
Vesicle Prep Pro publication in Nature Scientific Reports (2017)
Authors:
Shapovalov G., Ritaine A., Bidaux G., Slomianny, C., Borowiec A-S., Gordienko D., Bultynck G., Skryma R., Prevarskaya N.
2016 - Pore architecture of TRIC channels and insights into their gating mechanism
Port-a-Patch and Vesicle Prep Pro publication in Nature (2016)
Authors:
Yang H., Hu M., Guo J., Ou X., Cai T., Liu Z.
2015 - Golgi anti-apoptotic proteins are highly conserved Ion Channels That Affect Apoptosis and Cell Migration
Port-a-Patch and Vesicle Prep Pro publication in Journal of Biological Chemistry (2015)
Authors:
Carrara G., Saraiva N., Parsons M., Byrne B., Prole D.L., Taylor C.W., Smith G.L.