Succinate Dehydrogenase - Succinate-Coenzyme Q Reductase
Gene family: Mitochondrial complex II
The protein complex consists of four protein subunits: succinate dehydrogenase, (SDHA); succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, (SDHB); succinate dehydrogenase complex subunit C, (SDHC) and succinate dehydrogenase complex, subunit D, (SDHD)
The enzyme is found in the inner mitochondrial membrane in eukaryotes and the plasma membrane of many aerobic or facultative bacteria.
It catalyzes succinate oxidation in the citric acid cycle and transfers the electrons to quinones in the membrane, thus constituting a part of the aerobic respiratory chain (known as complex II). Complex 2 is a parallel electron transport pathway to complex 1, but unlike complex 1, no protons are transported to the intermembrane space in this pathway. Therefore, the pathway through complex 2 contributes less energy to the overall electron transport chain process.
Succinate Dehydrogenase: Background Information
CaV2.1, also known as brain calcium channel I, gives rise to P and/or Q-type calcium currents and is predominantly expressed in neuronal tissue. Localized to the presynaptic membrane of many types of neurons, CaV2.1 ion channels are involved in triggering neurotransmitter release via both, vesicle release and postsynaptic calcium entry.
Pre-synaptic Ca2+ influx and neurotransmitter release in neurons, fast synaptic transmission
Migraine (FHM-1), ataxia (EA-2, SCA6), Benign paroxysmal torticollis of infancy, hemiplegia
β4 subunit CACNB4, α2δ subunits, calmodulin, CaBP1, VILIP, mint, CASK, Syntaxin, SNAP25
Kuratoxin, ω-agatoxin IIIA, ω-conotoxin MVIIC, ω-agatoxin-IVA, roscovitine
Patch Clamp: whole cell, room temperature
CaV channels often show a rundown phenomenon. Adequate intra- and extrcellular solutions are essential for a good data quality.
Catterall WA, Perez-Reyes E, Snutch TP, Striessnig J (2005). "International Union of Pharmacology. XLVIII. Nomenclature and structure-function relationships of voltage-gated calcium channels". Pharmacol Rev. 57 (4): 411–25.