TRPA1 - Application of AITC and HC030031

130218 AITC HC 96icon sp96   SyncroPatch 96 (a predecessor model of SyncroPatch 384PE) data and applications: 
Cells were kindly provided by EMD Milipore.

Screenshot of online analysis data of hTRPA1 expressing HEK 293 cells as recorded on one 4x hole NPC-96 patch clamp chip. Currents were obained by performing ramps from -50 mV to +50 mV. Graphs show the summation of the current amplitudes of 4 cells per well, which were activated by 3 μM AITC and inhibited by 30 μM HC030031 plotted against time. For highlighted experiments, note the blue shade overlaid on the curves represents the full block with HC. The grey shade represents the activation step, the white color the wash steps. 

 

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