TRPC5 | Transient Receptor Potential Cation Channel Subfamily C Member 5

Transient  receptor potential channels


Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations

TRPC5: Background information

TRPC5, aslo called Short transient receptor potential channel 5 or transient receptor protein 5 is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. TRPC5 has been implicated in the mechanism of mercury toxicity and neurological behavior


Human Protein:
UniProt Q9UL62

Brain, adult heart, testis, ovaries

Function/ Application:
Pressure sensing, TRPC5 counteracts TRPC1 in regulating neurite outgrowth and appears to be involved in growth cone collapse that occurs when the growth cone detects a repellant factor

Hypertrophic Pyloric Stenosis

TRPC1, TRPC3, TRPC4, calmodulin, CABP1, enkurin, Na+–H exchange regulatory factor, interferon-induced GTP-binding protein, ring finger protein 24, SEC14 domain and spectrin repeat-containing protein 1, Calcium channel, voltage-dependent, gamma subunit, Mitochondrial calcium uniporter 3


Patch Clamp: whole cell


Ligand-gated ion channel, as well as mechano-sensitive ion channel

Recommended Reviews:
International Union of Pharmacology. XLVII. Nomenclature and Structure-Function Relationships of Voltage-Gated Sodium Channels. Pharmacol Rev 57: 397–409, Catterall, et al. 2005 

Data and Applications

TRPC5 - Block by Gadolinium

TRPC5icon pl   Patchliner data and applications: 
Data are taken from Milligan C.J. et al., Nature Protocols, 2009, 4(2), 244-255.

Current voltage relationships showing activaion of TRPC5 by extracellular application of Gd3+ (100 μM). Voltage ramps (−100 mV to 100 mV, holding potential 0 mV) were for 1s at 0.1 Hz.

Application Notes

TRPC5 - "Internal perfusion of Ca2+ to activate hTRPC5 on Nanion's SyncroPatch 384PE"

icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) application note   logo pdf   (1.7 MB)
Cells were kindly provided by Charles River.


2017 - lnvestigation of the Ion Channels hTMEM16A/Ano1 and TRPC5 and their Modulation by Intracellular Calcium

icon sp96   SyncroPatch 384PE (a predecessor model of the SyncroPatch 384) poster, BPS Meeting 2017  logo pdf   (1.3 MB)


2022 - Further Exploration of the Benzimidazole Scaffold as TRPC5 Inhibitors: Identification of 1-Alkyl-2-(pyrrolidin-1-yl)-1H-benzo[d]imidazoles as Potent and Selective Inhibitors

icon sp96  SyncroPatch 384PE (a predecessor model of the SyncroPatch 384 instrument) Publication in ChemMedChem (2022)

Sharma S., Pablo J., Tolentino K., Gallegos W., Hinman J., Beninato M., Asche M., Greka A., Hopkins C.

2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast

Icon Orbit Mini   Orbit mini publication in Cells (2019)

Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.

2019 - Design, synthesis and characterization of novel N-heterocyclic-1-benzyl-1H-benzo[d]imidazole-2-amines as selective TRPC5 inhibitors leading to the identification of the selective compound, AC1903

icon sp96   SyncroPatch 384PE article in Bioorganic and Medicinal Chemistry Letters (2019)

Sharma, S.H., Pablo J.L., Montesinos, M.S., Greka, A., Hopkins, C.R.

2011 - Rapid and Contrasting Effects of Rosiglitazone on Transient Receptor Potential TRPM3 and TRPC5 Channels

icon pl  Patchliner publication in Molecular Pharmacology (2011)

Majeed Y., Bahnasi Y., Seymour V.A.L., Wilson L.A., Milligan C.J., Agarwal A.K., Sukumar P., Naylor J., Beech D.J.

2009 - Robotic multiwell planar patch-clamp for native and primary mammalian cells

icon pl  Patchliner publication in Nature Protocols (2009)

Milligan C.J., Li J., Sukumar P., Majeed Y., Dallas M.L., English A., Emery P., Porter K.E., Smith A.M., McFadzean I., Beccano-Kelly D., Bahnasi Y., Cheong A., Naylor J., Zeng F., Liu X., Gamper N., Jiang L., Pearson H.A., Peers C., Robertson B., Beech D.J.

2008 - TRPC channel activation by extracellular thioredoxin

icon pl  Patchliner publication in Nature (2008)

Xu S.Z., Sukumar P., Zeng F., Li J., Jairaman A., English A., Naylor J., Ciurtin C., Majeed Y., Milligan C.J., Bahnasi Y.M., Al-Shawaf E., Porter K.E., Jiang L.H., Emery P., Sivaprasadarao A., Beech D.J.

2008 - Production of a specific extracellular inhibitor of TRPM3 channels

icon pl  Patchliner publication in British Journal of Pharmacology (2008)

Naylor J., Milligan C.J., Zeng F., Jones C., Beech D.J.

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