TRPM7 | Transient Receptor Potential Cation Channel Subfamily M Member 7
Family:
Transient receptor potential channels
Subgroups:
TRPC (TRPC1–TRPC7), TRPV (TRPV1-TRPV6), TRPA1, TRPM (TRPM1–TRPM8), TRPP (TRPP1–TRPP3, PKD1, PKDREJ, PKDL1–PKDL3), TRPML (TRPML1–TRPML3), TRPN
Topology:
Most TRP channels are composed of 6 transmembrane domains (helices) with intracellular N- and C-termini, non-selectively permeable to various cations
TRPM7: Background information
TRPM7 is a divalent cation channel permeable to calcium and magnesium. The protein encoded by this TRPM7 is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis.
Gene:
TRPM7
Human Protein:
UniProt Q96QT4
Tissue:
Widely expressed in mammalian cells, especially heart, pituitary, bone, adipose tissue, highest expression level in kidney
Function/ Application:
TRPM7 has a central role in magnesium ion homeostasis and in the regulation of anoxic neuronal cell death. Furthermore, the channel is involved in TNF-induced necroptosis downstream of MLKL by mediating calcium influx and it may be involved in a fundamental process that adjusts plasma membrane divalent cation fluxes according to the metabolic state of the cell.
Pathology:
Ophthalmomyiasis, Amyotrophic Lateral Sclerosis-Parkinsonism/Dementia Complex 1, dementia, lateral sclerosis, cardiac fibrosis, atrial fibrillation, macrothrombocytopenia. TRPM7 plays a role in cancer cells including survival, cell cycle progression, proliferation, growth, migration, invasion, and epithelial-mesenchymal transition (EMT)
Interaction:
PLCB1, PLCB2, phosphorylates annexin 1, forms complexes in synaptic vesicles with synapsin I and synaptotagmin I and directly interacts with snapin, TRPM7 forms heterodimers with TRPM6.
Modulator:
carvacrol, H+ (extracellular), ADP, naltriben, PiP2, 2-APB, spermine, fingolimod, La3+
Assays:
Patch Clamp: whole cell
Recommended Reviews:International Union of Pharmacology. XLIII. Compendium of voltage-gated ion channels: transient receptor potential channels., Pharmacol Rev 55(4):591-6 Clapham, et al. 2003
Data and Applications
TRPM7 - Current Traces
Port-a-Patch data and applications:
Shown are mTRPM7 (HEK293) raw current responses to a voltage ramp voltage procotol (−100 mV to 100 mV over 200 ms) recorded under control conditions and after subsequent internal perfusion with Mg2+ (left). Initally, currents showed some run-up before they became stable. The arrows in the timecourse on the right mark the timepoints for which the raw current traces are shown.
Application Notes
TRPM7 - "TRPM7 activation by internal sequestering of Mg2+ ions recorded on the Port-a-Patch"
Port-a-Patch application note: 
(0.8 MB)
Publications
2016 - Human T cells in silico: Modelling their electrophysiological behaviour in health and disease
Patchliner publication in Journal of Theoretical Biology (2016)
Authors:
Ehling P., Meuth P., Eichinger P., Hermann A.M., Bittner S., Pawlowski M., Pankratz S., Herty M., Budde T., Meuth S.G.