Patchliner application note: (0.4 MB)
Cells were kindly provided by Charles River.
TRPM8 is a member of the transient receptor potential channel (TRP) family. TRPM8 is known to be a thermosensitive channel, activated by cold temperatures (below ~25˚C) and ligands such as menthol, Eucalyptol and icilin. It belongs to the melastatin subfamily of TRP channels and shows an outward rectification with a relatively high permeability for calcium ions and little selectivity between monovalent cations. Menthol, a secondary alcohol produced by the peppermint herb, Mentha piperita, is widely used in the food and pharmaceutical industries as a cooling/soothing compound and odorant. It induces Ca2+ influx in a subset of sensory neurons from dorsal root and trigeminal ganglia, where the TRPM8 channel is specifically expressed. Here we present data of hTRPM8 collected on the Patchliner. Cells performed well on the Patchliner with a success rate of 89% for seal resistance >600 MΩ. Channel activation by the agonists menthol and Eucalyptol is shown.