21.07.2009 | New Patchliner Application Note: Heat activation of TRPV1
MUNICH, GERMANY, July 21, 2009 -- Nanion’s Patchliner® allows temperature controlled experiments, either at physiological temperatures or by application of temperature jumps.
Here, TRPV1 receptors were activated by noxious temperatures (>42 ˚C) using the temperature regulation feature of the Patchliner®. Solution were heated to temperatures up to 60 ˚C in the pipette and rapidly applied to the cell, transiently activating TRPV1 channels. The temperature responses were compared to capsaicin activation, and could also be blocked by co-application of antagonist and tempered solutions.
Download the TRPV1 application note here.