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16.12.2010 | Joint publication of the LMU and Nanion in Science Signaling

MUNICH, GERMANY, December 16, 2010 -- Researches from the LMU and Nanion Developed a Planar Patch Clamp Approach to Characterize Ionic Currents from Intact Lysosomes

Since its launch in the early 1980s, the patch clamp method has been extensively used to study ion channels in the plasma membrane, but its application to the study of intracellular ion channels has been limited. Unlike the plasma membrane, intracellular membranes are usually not stable enough to withstand mechanical manipulation by glass electrodes during seal formation and rupturing of the membrane. To circumvent these problems, we developed a method involving the immobilization of isolated organelles on a solid matrix planar glass chip. This glass chip contains a microstructured hole that supports the formation of gigaseals and subsequent electrophysiological recordings despite the high fragility of intracellular membranes.

In a collaboration between the Teams of Prof. Christian Wahl-Schott and Prof Martin Biel from the LMU and Dr. Andrea Brüggemann from Nanion Technologies it was possible to develop a method, which allows to record the ionic currents from intact Lysosomes. The detailed Method is published in Science Signaling, 7 December 2010.

We want to continue with this successful collaboration and further develop this method for other organelles, says Andrea Brüggemann.

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