"Electrophysiological investigation of integral membrane proteins using the Orbit mini – experiments on lipid bilayers made easy"
Date: 17. September 2020
Time: 4:00 PM CET / 10:00 AM EDT
Dr. Conrad Weichbrodt
A free standing lipid bilayer separating two aqueous compartments represents a fundamental prerequisite for the investigation of electrophysiological features of membrane spanning proteins like ion channels, porins or certain membrane active toxins. The convenient and reproducible preparation of these model bilayers and the often tedious workflow of conducting such an experiment on classic one channel setups, however, still remain an obstacle for easy and fast data generation.
Here we present Nanion’s Orbit mini device which is explicitly designed to meet the special requirements of experiments on artificial lipid bilayers: use of Ionera’s MECA (micro electrode cavity array) chip technology combined with state of the art low noise amplifiers (Elements S.R.L.) enable the fully parallel low-noise recording of four separate lipid bilayers at bandwidths up to 100 kHz. Today’s webinar consists of a general introduction of the system and a brief overview of it features, applications and optional add ons. We then demonstrate how to actually perform an experiment on the device showcasing translocation of Polyethylene glycol (PEG) polymers through the well established and commercially available porin alpha-Hemolysin.
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