CardioExcyte 96 – 结合细胞收缩,电生理与细胞活力记录
CardioExcyte 96非标记多功能心肌细胞研究系统是一款可同时记录心肌细胞网络收缩与电生理的设备，并且可以持续记录细胞基础阻抗并自动模拟急性与慢性细胞活力变化，可灵敏的记录可收缩的心肌细胞的细胞毒理反应，也可以记录一些非收缩的细胞，比如类肝细胞或癌细胞。CardioExcyte 96是一款全自动的设备，可同时记录96个孔，可提供高分辨率、非侵入与非标记的阻抗与场电位记录，CardioExcyte 96的主要特点有:
- 新的可选附件！FLEXcyte 96记录更真实的细胞收缩!
- 记录同一个孔的阻抗 & 胞外场电位
- 96孔平行记录
- 非侵入 & 非标记测量技术
- 高分辨率
- 可通过电压与光刺激进行细胞跳动同步
- 可进行急性与慢性细胞活力与毒理研究
- 为CiPA而验证的设备
CardioExcyte 96的96孔记录板 耗材整合的电子元件与成熟的芯片技术，使得该设备成为一款一站式的进行高效阻抗与胞外场电位记录的设备。CardioExcyte 96系统包括一套孵育系统，可以摆脱对孵育箱的依赖使得该系统可以直接放在实验台上，并能控制细胞环境的温度、空气成分和湿度。
CardioExcyte 96系统配有高效且易用的软件包，可方便地处理与导出数据，该系统已经在所有的主要干细胞供应商提供的干细胞来源心肌细胞上验证过，包括单层细胞与跳动的3D细胞簇;同时也在细胞系上 (肝细胞样细胞、癌细胞等)进行细胞毒理研究。
更多信息:
The CardioExcyte 96 package contains an Integra Viaflo Assist that provides automated multichannel pipetting for reproducible 96 well plate handling of cells and compounds.
The CardioExcyte 96 package includes an Incubation System. This means that the measurements are performed outside the cell culture incubator and the complete setup fits on a lab benchtop. The CardioExcyte 96 Incubation System controls temperature, gas mix and humidity.
Optical stimulation and investigation of impedance
and extracellular field potentials in parallel:
The stimulating optical lid, CardioExcyte 96 SOL, uses
LEDs for spatially uniform stimulation of cells transfected
with light-gated ion channels such as Channelrhodopsin2
(ChR2). After transfection of iPS cardiomyocytes with ChR2 the
cells can be stimulated by blue light. Each light impulse
induces a depolarization of the cells and thus an action potential is triggered. The advantages of optical
stimulation over electrical stimulation include the highly
precise timing, all cells of the beating network are
stimulated exactly at the time of the light stimulus. In
contrast, electrical stimulation propagates from the
electrode across the well and thus cells are stimulated
progressively. A mean beat calculation of precisely
timed beats enables in-depth compound analysis and
concentration response dependencies to be obtained.
This is one of the key software capabilities of the
CardioExcyte Control/ DataControl software package
that is provided with each CardioExcyte 96 system.
The CardioExcyte Control software supports online analysis of beating parameters. The unique Mean Beat Function automatically visualizes the average of beat traces from one well, enveloped by the standard deviation, indicating the consistency of the beat pattern within the recording well, i.e. if the cellular network is synchronously beating or not. The control software is intuitive and easy-to-use, at the same time very powerful in terms of experimental setup and online visualization of the recorded data. The DataControl 96 software package is an independent software package to load and analyze data recorded on the CardioExcyte 96.The analysis template can be saved and reloaded. Customized export formats of analysis results are integrated. The Online Analysis Post Processor (OAPP) is a software tool enabling quick visualization of well-to-well
statistics and concentration dependent effects. Parameters and traces can be normalized to control values or time points. Export of graphics is straightforward making OAPP a highly versatile tool for the analysis routine.
Hans-Peter Scholz, Technician,
NSP-96芯片是Nanion公司适配CardioExcyte 96的专利产品，有适用于不同实验的多种规格可供选择。
其他规格可根据要求提供！ Prof. Bjorn C. Knollmann, Director of the Center for Arrhythmia Research and Therapeutics of Vanderbilt University School of Medicine: Dr. Marc Rogers, CSO Hans-Peter Scholz, Technician, Dr. Ralf Kettenhofen, Head of Laboratory Dr. Daniel Konrad Anika Duenbostell-Schmidt, Head QC at Ncardia Block of NCX1 expressed in iCell® Cardiomyocytes2 by SEA 0400 caused a dose dependent increase in beat rate. The image on the left hand side displays the results of the blocking effect of Sotalol on hERG. The result is in good agreement with manual patch clamp data (Crumb et al., 2016). The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 10 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility measurements. The image on the left hand side displays the results of the blocking effect of Vandetanib on hERG, NaV1.5, CaV1.2 and KV4.3. The compound induced arrhythmia when iPSC-CM were exposed to a minimum concentration of 1 µM. Arrhytmic events were both detected in field potential recordings as well as in the impedance based contractility. Nanion developed a CiPA conform analysis for the Myocyte phase II study. The feature comes along is included in our CiPA analysis routine. Automated arrhythmia detection is just one highlight out of many when it comes to the CardioExcyte 96 software. ChR2 transfected Cor.4U cells are following the optical pace rate. Raw data traces upon a 1 Hz, 1.5 Hz, 2 Hz., 2.5 Hz and 3 Hz stimulation rate, extracellular field potentials (top) and impedance (bottom). The stimulating optical lid, CardioExcyte 96 SOL, uses LEDs for spatially uniform stimulation of cells transfected with light-gated ion channels such as Channelrhodopsin2 (ChR2). Right graph: LPM – Light pulse per minute plotted against the recorded beat rate (average of 96 wells). ChR2 transfected Cor.4U cells are following the optical pace rate. Murine mammary carcinoma cells (H8N8 and H8N8 T3.2) were monitored over a time period of 500 h. Changes in impedance, and therefore confluency, were used as a measure of toxicity. (A) Impedance (left) and EFP signals (right) in control conditions Impedance (left) and EFP signals (right) in control conditions (A) and 1 μM sotalol (B) are shown. A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in isoproterenol at the concentrations indicated. Isoproterenol acts as a heart stimulant, it increases heart rate, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid. In the presence of even low concentrations of isoproterenol (1 nM), an increase in the beat rate is observed. B Bar graph showing beat rate before (dark blue) and after (light blue) incubation in isoproterenol at the concentrations indicated. At all concentrations, a clear increase in beat rate is observed. C EFP signal of 12 wells in control conditions (left) and the same 12 wells after 30 mins incubation in isoproterenol at the concentrations indicated (right). Isoproterenol shortens the FPD, an effect which is highlighted in the inset where the trace in the presence of isoproterenol (300 nM; black) and the control trace (red) are shown overlaid. Effect of the specific hERG blocker, E4031, on the impedance and EFP signals. A Impedance signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031 (300 nM, 3 mins incubation) induces EAD, shown in the inset, which can lead to potentially fatal ventricular arrhythmias. B EFP signal of 12 wells in control conditions (left) and the same 12 wells after 13 mins incubation in E4031 at the concentrations indicated (right). E4031(300 nM, 30 mins incubation) also causes arrhythmic effects in the EFP mode shown in the inset. Nifedpinie is a dihydropyridine calcium channel blocker that primarily blocks L-type calcium channels. Impedance and EFP recordings on Cor.4U cells reveal a concentration dependent effect on impedance amplitude, beat rate and also a shortening of the FPD as expected. Impedance amplitude is not changed by addition of increasing concentrations of Tetracaine (left panel), while beat rate of Cor.4U® cells is decreasing. For example, 29.6µM of Tetracaine decreased the beat rate by ~60% when compared to pre-addition values. Cumulative dose-response relationships indicate Tetracaine potency for same-well additions. Representative raw traces for impedance signals (middle panel) clearly indicate a decrease in cell monolayer beat rate with increasing concentrations of Tetracaine. Time-dependent effect of Pentamidine on Cor.4U cardiomyocytes. Pentamidine clinically causes acquired long QT syndrome, which is associated with prolonged QT intervals, tachycardias, and sudden cardiac arrest. Pentamidine delays terminal repolarization in human heart by acutely blocking cardiac inward rectifier currents. At the same time, it reduces surface expression of the cardiac potassium channel IKr/human ether à-go-go-related gene (hERG). The CardioExcyte 96 allows for The impedance signal recorded on the CardioExcyte 96 changes as a result of alterations in confluency, cell contact (morphological shape) and conductivity of adherent cells and thereby provides a measure of toxicity. MH cells from MetaHeps® were grown on NSP-96 plates and base impedance was monitored over time. Increasing concentrations of paracetamol induce a decrease in base impedance of MH cells which can be monitored continuously. Tween (2%) induced 100% cell death and was used as a positive control.
Date: March 17. 2020, 4:00 PM CET (11:00 AM EDT) The Webinar focuses on the FLEXcyte 96 technology, an Add-on of the CardioExcyte 96 instrument. The analysis of cardiomyocyte contractility as well as data of short-termed and long-termed compound applications is discussed. Speakers: 日期: 9月12日, 4:00 PM CEST (北京时间10:00 PM) 获取CiPA肌细胞与离子通道工作组的最新进展： This Webinar covers the introduction of the CardioExcyte 96, a hybrid device for impedance- and MEA-type recordings from intact, beating networks of stem cell-derived cardiomyocytes. Presenter: Presenter: Presenter: Short introduction on the CardioExcyte 96 (tutorial video, 3 minutes)附件与特征
CardioExcyte 96 平台
CardioExcyte 96 培养系统
CardioExcyte 96 光遗传附件
软件
CardioExcyte 96 软件包
Statement about the DataControl 96 Package
Hans-Peter Scholz - Statement about the Analysis Software of the CardioExcyte 96 "DataControl 96"
“DataControl 96 allows us to analyze large amounts of data and represents a huge step towards standardized experiment analysis. All the well's individual raw data, analysis results, concentration response curves and also the fits averaged per compound are displayed on a single screen, which means that all relevant information is right at the place where we want to see it. Analysis templates can be used to re-do the same analysis procedures on a different compound, but same target, thus reducing the effort for data handling to just a couple of mouse clicks. Powerful averaging with standard deviation display and referencing methods allow for great quality control. Furthermore, multiple export options are available to allow a seamless integration in databases.”
Biopharma, Global Early Non Clinical Safety, Merck KGaA, Darmstadt, Germany耗材
NSP-96
描述与材质
可选规格
CardioExcyte 96 and 
FLEXcyte 96 product flyer on plates available 
(1.3 MB) 用户评价与案例研究
Prof. Bjorn C. Knollmann - Statement about the CardioExcyte 96
“We are happy to have acquired the CardioExcyte 96 from Nanion and look forward to publishing the first of many papers. We looked at several different devices on the market but were impressed by the resolution and ease-of-use of the CardioExcyte 96 and Nanion’s enthusiasm and friendly customer support. I was particularly intrigued by the pacing feature, the single electrode design giving better signal to noise ratio, the dual EFP + impedance capability, and the environmental chamber so that you can run everything on the benchtop. The new optical pacing feature added in 2017 has further expanded the utility of the instrument for drug testing and disease modeling: For the first time, rate-dependent changes of cardiac electrophysiology and contractility can be easily quantified.”
Marc Rogers - Statement about the Patchliner and CardioExcyte 96
"It has been an absolute pleasure and privilege to work with Niels and the Nanion team over the past decade. I firmly believe that our two small, but independently minded ion channel focused companies, share a similar business ethos and a clear focus on delivering cost-effective solutions to our customers. In our extensive use of the Patchliner platform since 2007, my teams have benefited from the dedication of Nanion’s service engineers, application scientists, and bespoke data acquisition routines, whilst Nanion have benefited from seeing their products applied and adapted to real-world drug discovery projects and assays." "This fruitful working relationship has now been extended to our use of the CardioExcyte96 phenotypic assay platform, which we utilise to develop and validate human iPSC cardiomyocyte cell lines and assays to meet the post-hERG, predictive cardiac safety testing needs of the drug discovery industry”."
Metrion Biosciences
Hans-Peter Scholz - Statement about the Analysis Software of the CardioExcyte 96 "DataControl 96"
“DataControl 96 allows us to analyze large amounts of data and represents a huge step towards standardized experiment analysis. All the well's individual raw data, analysis results, concentration response curves and also the fits averaged per compound are displayed on a single screen, which means that all relevant information is right at the place where we want to see it. Analysis templates can be used to re-do the same analysis procedures on a different compound, but same target, thus reducing the effort for data handling to just a couple of mouse clicks. Powerful averaging with standard deviation display and referencing methods allow for great quality control. Furthermore, multiple export options are available to allow a seamless integration in databases.”
Biopharma, Global Early Non Clinical Safety, Merck KGaA, Darmstadt, Germany
Dr. Ralf Kettenhofen - Statement about the CardioExcyte 96
"CardioExcyte 96 is an easy-to-use system, providing impedance-based and MEA-like cardiac safety data from a diversity of stem cell-derived cardiomyocytes, and constitutes an excellent complement to automated patch clamp-based safety screening. Concentration- and time-dependence of a compound’s potential cardiotoxicity can efficiently be obtained where the alteration of beating patterns can give a hint as to which cardiac ion channel(s) is affected, after which detailed electrophysiology investigations can be undertaken. Cardiac network responses offer a comprehensive view of a compound’s safety profile, without having to use in-vivo methods, which saves time, costs and suffering. Further on, the powerful software, used for recordings and analysis, employs comprehensive beat investigation algorithms, displaying detailed beating kinetics in real-time. Data handling and export is straightforward, easy to grasp and yet very, very powerful."
Ncardia, Cologne, Germany.
Dr. Daniel Konrad - Statement about the CardioExcyte 96
“Impedance and field potential data of beating cardiomyocytes is getting increasingly important to obtain a comprehensive view of the cardiac liability of test compounds. To answer these questions for our clients, B’SYS successfully implemented the CardioExcyte 96 system and provides related validated services to its customers, mostly using ipS derived cardiomyocytes. B’SYS’ scientists are pleased with the CardioExcyte’s ease of operation and data acquisition. The powerful software allows for applying meaningful data analysis and time saving export of data as needed for our daily customer reports.“
CEO B'SYS
Anika Duenbostell-Schmidt - Statement about the CardioExcyte 96
"I use the CardioExcyte 96 weekly to test substances on our human iPSC-derived cardiomyocytes. It allows me to map, compare and evaluate a wide variety of pharmacological responses with a high degree of reliability and uniformity, and evaluate them through the rapid analysis function."
Ncardia, Cologne, Germany.数据与应用
NCX1 - Effect of block of cardiac NCX on beat rate
CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.
Cardiac Ion Channels - Pharmacology of Sotalol
CardioExcyte 96 and 
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.
Cardiac Ion Channels - Pharmacology of Vandetanib
CardioExcyte 96 and Patchliner data and applications:
Cells were kindly provided by Charles River and Cellular Dynamics.
Cardiomyocytes - Myocyte phase II study: CiPA conform analysis and arrhythmia detection
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Cardiomyocytes - Channelrhodopsin 2 (ChR2) transfected Cor.4U cells and optical pacing
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Cardiomyocytes - Optogenetics meets cardiac safety
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Murine Mammary Carcinoma Cells - Effect of CAF (cyclophosphamide, adriamycin (doxorubicin) and 5-fluouracil)
CardioExcyte data and applications:
Data kindly provided by Oliver Reinhardt, MPI Experimentelle Medizin, Göttingen
H8N8 cells have been developed as a tool to investigate tumor cell recurrence, chemoresistance and epithelial-to-mesenchymal transition (EMT). Cells were treated with increasing concentrations of CAF (cyclophosphamide, adriamycin (doxorubicin) and 5-fluouracil) on the CardioExcyte 96 during impedance measurements. Therefore, the CardioExcyte 96 in combination with murine mammary carcinoma cells provides a novel tool for investigating therapy resistance of cancer cells in vitro.
Data kindly provided by Oliver Reinhardt, MPI Experimentelle Medizin, Göttingen
Cardiomyocytes - Effects of verapamil on impedance (left) and EFP (right) signals of Cellartis Cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Takara Bio Cellartis Clontech.
(B) Upon application of 10 nM verapamil
(C) Upon application of 100 nM verapamil.
(D) The mean beats for impedance (left) and EFP (right) are shown in D
Cardiomyocytes - Effects of sotalol on impedance (left) and EFP (right) signals of Cellartis Cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Takara Bio Cellartis Clontech.
Irregular beating can be observed in the presence of sotalol.
Cardiomyocytes - Effect of the ß-adrenergic receptor agonist, Isoproterenol, on the impedance and EFP signals on iCells
CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.
Cardiomyocytes - Effect of E4031 on the impedance and EFP signals on iCell cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Cellular Dynamics.
Cardiomyocytes - Nifedipine and its concentration dependent effect on Cor.4U cells
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Cardiomyocytes - Tetracaine dose response curves as recorded with Cor.4U cells
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Extracellular Field Potential (EFP) spike amplitude is decreased by cumulative Tetracaine dose applications to the same monolayer of Cor.4U® cardiomyocytes (top right), in agreement with compound mechanism of action. Representative raw traces for EFP signals (bottom graph) clearly indicate a decrease in spike amplitude.
Cardiomyocytes - Time-dependent effect of Pentamidine on Cor.4U cardiomyocytes
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis.
Insets: EFP raw traces and online analysis value FPDc in the presence of Pentamidine (10 µM) at different timepoints.
Cardiomyocytes - Data from different cell providers
CardioExcyte data and applications:
Cells were kindly provided by Axiogenesis, Cellular Dynamics, GE Healthcare, Pluriomics, ReproCell, Takara Bio Cellartis Clonetech.
• Non-invasive, label-free measurements of beating cardiomyocyte networks
• 96 recording wells in parallel with 1 ms time resolution
• Quick experiments or long-term compound effects on cardiotoxicity
• Real-time access to beating parameters
• Outstanding software for data analysis and export
• Cost effcient consumables - 96-well format
Hepatocytes - Paracetamol toxicity on hepatocyte-like MetaHeps cells
CardioExcyte data and applications:
Cells were kindly provided by MetaHeps.网络研讨会与影像
Webinars
17.03.2020 | Webinar: A new technology to evaluate mature cardiac contractility on highthroughput level
FLEXcyte 96, CardioExcyte 96 Webinar
Dr. Sonja Stölzle-Feix
Dr. Matthias Gossmann
12.09.2018 | 在线研讨会: CiPA study: Bridging ion channel and myocyte data
CardioExcyte 96, Patchliner and SyncroPatch 384PE (a predecessor model of SyncroPatch 384i)
28.04.2015 | Webinar: Excited About Contraction – Combining Contractility and Excitability Measurements in Cardiotoxicity Screening
CardioExcyte 96
Movies: Tutorials and Oral Presentations
2018 - HTS Phase I study: an update on progress of the CiPA Ion Channel Work Stream using the SyncroPatch 384PE and Patchliner
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i), Patchliner and CardioExcyte 96 Oral Presentation
Tim Strassmaier, Nanion Technologies Inc. USA
Source:
Webinar: "CiPA study: Bridging ion channel and myocyte data", September 12, 2018
2018 - CiPA myocyte phase II validation study results: cross-site comparison using the CardioExcyte 96
CardioExcyte 96 Oral Presentation
Dr. Sonja Stölzle-Feix, Nanion Technologies
Source:
Webinar: "CiPA study: Bridging ion channel and myocyte data", September 12, 2018
2017 - Impedance-Based Monitoring of Cell-Based Assays: The Power of MultiFrequency Recordings
CardioExcyte 96 Oral Presentation
Prof. Dr. Joachim Wegener, University of Regensburg, Germany
2017 - CardioExcyte 96 Tutorial Video
CardioExcyte 96
下载:
Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors: Authors:
应用数据
Cardiac Ion Channels - "High Throughput Screening of Cardiac Ion Channels"
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) Patchliner CardioExcyte 96 application note (2.3 MB)
Cardiomyocytes - "Combining automated patch clamp, impedance and EFP of hiPSC-CMs"
CardioExcyte 96 SyncroPatch 3984PE (a predecessor model of SyncroPatch 384i) Patchliner Application Note
Cells kindly provided by Takara-Clonetech.
Cardiomyocytes - "Impedance and EFP recordings of Cor.4U cells using Nanion’s CardioExcyte 96"
CardioExcyte 96 Application Note (1.3 MB)
Cells were kindly provided by Ncardia.
Cardiomyocytes - "Impedance and EFP recordings of iCell Cardiomyocytes² on the CardioExcyte 96"
CardioExcyte 96 Application Note (2.8 MB)
Cells were kindly provided by Cellular Dynamics.
Cardiomyocytes - "Impedance and EFP recordings of Pluricyte Cardiomyocytes on the CardioExcyte 96"
CardioExcyte 96 Application Note (1.3 MB)
Cells were kindly provided by Ncardia.
Cardiotoxicity - "Assessing cardiotoxic risk of anti-cancer agents on Nanion’s CardioExcyte 96"
CardioExcyte 96 Application Note (0.6 MB)
Cells were kindly provided by Ncardia and experiments performed by Oliver Reinhardt and Dr. Frauke Alves from the University of Göttingen.
CytoSwitch - "Inducing cell death using a photostatin on the CardioExcyte 96 and SOL"
CardioExcyte 96 and SOL Application Note (0.4 MB)
Cells were kindly provided by LMU Munich/CytoSwitch.
Hepatocytes - "Investigating DILI using MetaHeps cells on Nanion’s CardioExcyte 96"
CardioExcyte 96 Application Note (0.7 MB)
Cells were kindly provided by Metaheps GmbH.
Hepatocytes - Toxicity analysis in expanded upcyte® liver cells on Nanion’s CardioExcyte 96
CardioExcyte 96 Application Note (7.8 MB)
Cells were kindly provided by upcyte technologies产品彩页
CardioExcyte 96 / FLEXcyte 96 Flyer - Plates
CardioExcyte 96 and FLEXcyte 96 Product flyer (1.3 MB)发表文献
2019 - Transcriptomic profiling reveals p53 as a key regulator of doxorubicin-induced cardiotoxicity
CardioExcyte 96 publication in Cell Death Discovery (2019)
McSweeney K.M., Bozza W.P., Alterovitz W.-L., Zhang B.
2019 - The patient-independent human iPSC model – a new tool for rapid determination of genetic variant pathogenicity in long QT syndrome
CardioExcyte 96 publication in Heart Rhythm (2019)
Chavali, N.V., Kryshtal, D.O., Parikh, S.S., Wang, L., Glazer, A.M., Blackwell, D.J., Kroncke, B.M., Shoemaker, M.B., Knollmann, B.C.
2019 - The N-termini of GRK2 and GRK3 simulate the stimulating effects of RKIP on β-adrenoceptors
CardioExcyte 96 publication in Biochemical and Biophysical Research Communications (2019)
Maimari T., Krasel C., Bünemann M., Lorenz K.
2019 - Pharmacological enhancement of repolarization reserve in human induced pluripotent stem cells derived cardiomyocytes
CardioExcyte 96 publication in Biochem. Pharmacol. (2019)
Treat JA, Goodrow RJ, Bot CT, Haedo RJ, Cordeiro JM.
2019 - Particulate matter 2.5 induced arrhythmogenesis mediated by TRPC3 in human induced pluripotent stem cell-derived cardiomyocytes
CardioExcyte 96 publication in Archives of Toxicology (2019)
Cai C., Huang J., Lin Y., Miao W., Chen P., Chen X., Wang J., Chen M.
2019 - Downregulation of miR-146a Contributes to Cardiac Dysfunction Induced by the Tyrosine Kinase Inhibitor Sunitinib
CardioExcyte 96 publication in Frontiers in Pharmacology (2019)
Shen L., Li C., Zhang H., Qiu S., Fu T., Xu Y.
2019 - An integrated characterization of contractile, electrophysiological, and structural cardiotoxicity of Sophora tonkinensis Gapnep. in human pluripotent stem cell-derived cardiomyocytes
CardioExcyte 96 publication in Stem Cell Research & Therapy (2019)
Wang R., Wang M., Wang S., Yang K., Zhou P., Xie X., Cheng Q., Ye J., Sun G., Sun X.
2018 - The opioid oxycodone use‐dependently inhibits the cardiac sodium channel Nav1.5
CardioExcyte 96 publication in British Journal of Pharmacology (2018)
Meents J.E., Juhasz K., Stölzle-Feix S., Peuckmann-Post V., Rolke R. Lampert A.
2018 - Simplified footprint-free Cas9/CRISPR editing of cardiac-associated genes in human pluripotent stem cells
CardioExcyte 96 publication in Stem Cells and Development
Kondrashov A., Hoang M.D., Smith J., Bhagwan J., Duncan G., Mosqueira D., Munoz M., Vo N.T.N., Denning C.
2018 - Safety Pharmacology Methods and Models in an Evolving Regulatory Environment
CardioExcyte 96 & CiPA Editorial in Journal of Pharmacological and Toxicological Methods (2018)
Pugsley M.K., Harter M.L., de Korte T., Connaughton C., Authier S., Curtis M.J.
2018 - Hypertrophic cardiomyopathy-linked mutation in troponin T causes myofibrillar disarray and pro-arrhythmic action potential changes in human iPSC cardiomyocytes
CardioExcyte 96 publication in Journal of Molecular and Cellular Cardiology
Wang L., Kim K., Parikh S., Cadar A.G., Bersell K.R., He H., Pinto J.R., Kryshtal D.O., Knollmann B.C.
2018 - Cross-site comparison of excitation-contraction coupling using impedance and field potential recordings in hiPSC cardiomyocytes
CardioExcyte 96 publication in Journal of Pharmacological and Toxicological Methods (2018)
Bot C.T., Juhasz K., Haeusermann F., Polonchuk L., Traebert M., Stölzle-Feix S.
2017 - Structural and electrophysiological dysfunctions due to increased endoplasmic reticulum stress in a long-term pacing model using human induced pluripotent stem cell-derived ventricular cardiomyocytes
CardioExcyte 96 publication in Stem Cell Research and Therapy (2017)
Cui C., Geng L., Shi J., Zhu Y., Yang G., Wang Z., Wang J., Chen M.
2017 - Frequency-Dependent Multi-Well Cardiotoxicity Screening Enabled by Optogenetic Stimulation
CardioExcyte 96 publication in International Journal of Molecular Sciences
Rehnelt S., Malan D., Juhasz K., Wolters B., Doerr L., Beckler M., Kettenhofen R., Bohlen H., Bruegmann T., Sasse P.
2017 - Combined Impedance and Extracellular Field Potential Recordings from Human Stem Cell-Derived Cardiomyocytes
CardioExcyte 96 book chapter in Stem Cell-Derived Models in Toxicology (2017)
Obergrussberger A., Thomas U., Stölzle-Feix S., Becker N., Juhasz K, Doerr L., Beckler M., George M., Fertig N.
2017 - An impedance-based approach using human iPSC-derived cardiomyocytes significantly improves in vitro prediction of in vivo cardiotox liabilities
CardioExcyte 96 publication in Toxicology and Applied Pharmacology (2017)
Koci B., Luerman G., Duenbostell A., Kettenhofen R., Bohlen H., Coyle L., Knight B., Ku W., Volberg W., Woska Jr. J.R., Brown M.P.
2016 - Safety pharmacology studies using EFP and impedance
CardioExcyte 96 publication in Journal of Pharmacological and Toxicological Methods (2016)
Obergrussberger A., Juhasz K., Thomas U., Stölzle-Feix S., Becker N., Dörr L., Beckler M., Bot C., George M., Fertig N.
2016 - Identification of Drug-Drug Interactions In Vitro: A Case Study Evaluating the Effects of Sofosbuvir and Amiodarone on hiPSC-Derived Cardiomyocytes
CardioExcyte 96 publication in Toxicological Sciences (2016)
Millard DC, Strock CJ, Carlson CB, Aoyama N, Juhasz K, Goetze TA, Stoelzle-Feix S, Becker N, Fertig N, January CT, Anson BD, Ross JD.
2015 - Novel screening techniques for ion channel targeting drugs
Patchliner, SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and CardioExcyte 96 publication in Channels (2015)
Obergrussberger A., Stölzle-Feix S., Becker N., Brüggemann A., Fertig N., Möller C.
2015 - New Easy-to-Use Hybrid System for Extracellular Potential and Impedance Recordings.
CardioExcyte 96 publication in Journal of Laboratory Automation (2015)
Doerr L., Thomas U., Guinot D.R., Bot C.T., Stoelzle-Feix S., Beckler M., George M., Fertig N.海报
2019 - High content in vitro cell monitoring effects of adjuvant chemotherapy in breast cancer and cancer treatment-related cardiomyopathy
CardioExcyte 96 poster, SOT Conference 2019 (0.8 MB)
2018 - Combining electrophysiology and contractility recordings for more complete assessment of hiPSC-CMs
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i), Patchliner and 
CardioExcyte 96 poster, Europhysiology Meeting 2018 (1.4 MB)
2018 - A Sensor Based Technique for Pharmacological Safety Testing of Cardiac Transport Proteins NCX, NaKATPase and Respiratory Chain Complexes
CardioExcyte 96 and 
SUFE²R 96SE poster, SPS 2018 (1.7 MB)
2018 - Assessment of Drug Effects on Cardiomyocyte Function: Comprehensive In Vitro Proarrhythmia Assay (CiPA) Results
CardioExcyte 96 poster, SOT Meeting 2018 (1.8 MB)
2018 - Optogenetic technologies enable high throughput ion channel drug discovery and toxicity screening
SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) and CardioExcyte 96 poster, Biophysics Annual Meeting 2018 (1.3 MB)
2018 - Investigations into idiosyncratic drug-induced hepatotoxicity and chronic proliferation of cancer cells using a label-free method
CardioExcyte 96 poster, Biophysics Annual Meeting 2018 (1.3 MB)
2016 - Next level toxicity screening: From single channel to overall cell behavior
Orbit mini, CardioExcyte 96 and SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) poster, Meeting of the French Society of Toxinology (SFET) 2015 (0.9 MB)
2015 - Complementary automated patch clamp, extracellular field potential and impedance recordings of iPSCs: safety screening tool box for the future
Patchliner and CardioExcyte 96 and SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) poster, SPS 2015 (2.7 MB)演讲文档
Dr. Marc Rogers - Statement about the CardioExcyte 96
“Validation of a impedance-based phenotypic screening assay able to detect multiple mechanisms of chronic cardiotoxicity in human stem cell-derived cardiomyocytes” (slide deck) (4.5 MB)
Metrion Biosciences is a UK-based Contract Research Organization (CRO) focussed on delivering a range of high quality ion channel screening and drug discovery services. We provide sophisticated electrophysiology assays to support medicinal chemistry optimisation programmes, CiPA-compliant cardiac safety
Download the material presented at the 64th Annual Meeting of the Biophysical Society below
2017 - Cross-site comparison of myocyte phase II compounds on different iPS cardiomyocytes based on CardioExcyte 96 recordings
CardioExcyte 96 presentation (slide deck) (2.2 MB)
2018 - Innovations for cell monitoring in safety and toxicity assays
CardioExcyte 96 & SyncroPatch 384PE (a predecessor model of SyncroPatch 384i) presentation (slide deck) (3.2 MB)
2018 - Optogenetic control of transiently transfected hiPSC-derived cardiomyocytes for the assessment of drug related cardiotoxicity
CardioExcyte 96 presentation (slide deck) (1.4 MB)
2017 - HTS in Cardiac Safety
CardioExcyte 96 presentation (slide deck) (4.0 MB)
