• CardioExcyte 96

    结合阻抗与类MEA记录
  • CardioExcyte 96

    用于心脏安全筛选
  • CardioExcyte 96

    下一代非标记细胞分析
  • CardioExcyte 96

    直观的数据分析&心律失常检测

2021 - Targeting of CAT and VCAM1 as Novel Therapeutic Targets for DMD Cardiomyopathy

Icon CE  CardioExcyte 96 Publication in Cell & Development Biology (2021)

Authors:
Li B., Xiong W., Liang W-M., Chiou J-S., Lin Y-J., Chang A.C.Y.

Journal: 
Cell & Development Biology (2021) doi: 10.3389/fcell.2021.659177


Abstract: 

Duchenne muscular dystrophy (DMD) related cardiomyopathy is the leading cause of early mortality in DMD patients. There is an urgent need to gain a better understanding of the disease molecular pathogenesis and develop effective therapies to prevent the onset of heart failure. In the present study, we used DMD human induced pluripotent stem cells (DMD-hiPSCs) derived cardiomyocytes (CMs) as a platform to explore the active compounds in commonly used Chinese herbal medicine (CHM) herbs. Single CHM herb (DaH, ZK, and CQZ) reduced cell beating rate, decreased cellular ROS accumulation, and improved structure of DMD hiPSC-CMs. Cross-comparison of transcriptomic profiling data and active compound library identified nine active chemicals targeting ROS neutralizing Catalase (CAT) and structural protein vascular cell adhesion molecule 1 (VCAM1). Treatment with Quecetin, Kaempferol, and Vitamin C, targeting CAT, conferred ROS protection and improved contraction; treatment with Hesperidin and Allicin, targeting VCAM1, induced structure enhancement via induction of focal adhesion. Lastly, overexpression of CAT or VCAM1 in DMD hiPSC-CMs reconstituted efficacious effects and conferred increase in cardiomyocyte function. Together, our results provide a new insight in treating DMD cardiomyopathy via targeting of CAT and VCAM1, and serves as an example of translating Bed to Bench back to Bed using a muti-omics approach.


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