Orbit 16 TC - 高通量脂双层工作站

Nanion公司的Orbit 16 TC 平台可轻松高效的进行脂双层涂抹与记录，该设备能自动一次性形成16个脂双层并对其进行完全平行记录，所以能快速地生成有效的数据。 Orbit 16 TC 主要特点有：

一键操作自动形成16个人工脂双层
完全平行的低噪音、高带宽记录
可同时记录16个脂双层从而获得大量的数据
低噪音16通道放大器，也可以兼容外置放大器
附带专用记录软件
目标蛋白可直接插入和通过蛋白脂质体插入
可记录: 电压、配体门控离子通道、温度敏感离子通道
也可记录纳米孔、抗菌肽、毒素等...
低实验成本的MECA 16 TC芯片

Orbit 16 TC 内置专业的低噪16通道放大器(Elements s.r.l.)，支持使用最高带宽同时记录16个脂双层。此外，Orbit 16 TC 还兼容绝大多数在售的放大器，包括Tecella公司的16通道Triton放大器、EPC-10或Axopatch等单通道放大器等。Orbit内置多路转接器，可以自动在记录位点之间切换从而实现用单通道放大器记录多个脂双层位点。

Orbit 16 TC 脂双层工作站

完整的Orbit 16 TC 系统包括主记录站、内置的Elements e16脂双层专用放大器和支持Ionera公司的MECA 16 TC 16记录芯片的记录模块。

更多信息:

软件

Orbit 16 TC EDR4 Software

eFOUR Software

The intuative and easy to learn EDR4 software for the Orbit 16 TC was developed by our partner Elements S.R.L.(Italy). Further detailed information can be found at https://elements-ic.com/

耗材

MECA 16 TC chips

The MECA 16 TC recording substrate contains an array of 16 circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is automatically formed by remotely actuated painting (Ionera1-SPREAD), thus roofing the liquid-filled cavity. The bilayers can be easily and repeatedly zapped and re-formed in an automated fashion. After bilayer formation, ion channels or nanopores are reconstituted via self-insertion, proteoliposome fusion or dilution from detergent micelles.

The MECA 16 TC recording chips are produced and quality assured by our partner Ionera Technologies GmbH in Freiburg Germany and shipped from Munich to our international customers. Different types of MECA 16 chips will be available depending on the sample.

Orbit 16 Meca Chip

Available chip types

"MECA 16 TC Recording Chips 100 µm": 16-well recording chip with 100 µm cavity size (Order # 131013)

用户评价与案例分析

Prof. Dr. Friedrich Simmel - Statement about the Orbit 16

Icon Orbit “The Orbit 16 enables us to generate high quality, single channel recordings with synthetic DNA membrane channels, which in our experience are notoriously difficult to measure. DNA pores are quite hard to functionally incorporate into lipid bilayers, but could be successfully investigated using the Orbit 16, as published in Science. The Orbit 16 offers a drastic increase in throughput since it substantially speeds up formation of bilayers and data generation by its parallel recording channels, thus providing us an easy-to-use platform for efficient and accurate research on DNA nanodevice-membrane interactions.”

Dr. Friedrich Simmel, Professor, Systems Biophysics and Bionanotechnology, Physics Department and ZNN/WSI
Technical University of Munich, Munich, Germany

Prof. Dr. Stefan Howorka - Statement about the Orbit 16

Icon Orbit “Within our research on the CsgG channel, Nanion’s Orbit 16 - combined with Ionera's MECA Chip technology - has substantially boosted our scientific output. The outstanding research tool is easy to handle and speeds up the parallel generation of 16 bilayers. By increasing the throughput of single-channel current recordings, it is a breakthrough in the biophysical analysis of pore forming proteins. Within approximately one week’s worth of lab time, we had the data needed for the recent paper in Nature. This would have been hard to achieve using conventional serial bilayer methods. In addition, the ease-of-use provided by the Orbit 16 shortens the learning curve for making high quality bilayer recordings. As a benefit in academia, students can now get hands-on experience with bilayer recordings also for shorter projects.“

Dr. Stefan Howorka, Associate Professor of Organic Chemistry and Chemical Biology
University College London, London, UK

数据与应用

Alpha-Hemolysin - Parallel Recordings of monoPEG-28 Block

Icon Orbit Orbit 16 data and applications:
Data courtesy of Dr. Gerhard Baaken et. al., University of Freiburg / Ionera.

Event-averaged histograms (black) and overlaid current traces (blue) of parallel and simultaneous recordings on a MECA chip of monoPEG-28-mediated blockages of hemolysin nanopore(s). The current traces were recorded with a multichannel amplifier (Tecella Jet 16). Histograms were derived from the mean current levels of at least 2000 visits of blocked stated per cavity (20 kHz sample frequency).
Read the full paper. (Am. Chem. Soc Nano, 5(10), 8080-8088, 2011)

Alpha-Hemolysin - Block by Mono- and Poly PEGs

Icon Orbit Orbit 16 data and applications:
Data courtesy of Dr. Gerhard Baaken et.al, University of Freiburg / Ionera.

Current traces and histograms derived from recordings of αHL pores blocked by monoPEG-28 and polyPEG-1500 on an Ionera MECA chip (AxoPatch 200B, filter freq: 20kHz, digitized at 200 kHz).
Read the full paper: (Am. Chem. Soc Nano, 5(10), 8080-8088, 2011)

Alamethicin - Parallel recordings

Icon Orbit Orbit 16 data and applications:
Data courtesy of Dr. Gerhard Baaken, University of Freiburg / Ionera.

The data image shows parallel recordings from reconstituted alamethicin channels. See also the paper: "Alamethicin Supramolecular Organization in Lipid Membranes from ¹⁹F Solid-State NMR", Salnikov et al. (2016) Biophysical Journal 111(11): 2450-2459.