Orbit mini - 脂双层工作站
- 可选的温度控制附件 - 自动制冷与加热
- 可记录: 电压、配体门控离子通道、温度敏感离子通道
Orbit mini包含一个内置的小型化四通道放大器，可在高带宽下进行低噪音记录而不需要其他设备辅助 – 可以在任何环境下进行实验。此外独特的设计使得测量室的温度可以自由控制而不会导入额外的噪音。
完整的Orbit mini平台包含内置四通道放大器(Elements s.r.l.)的记录单元和选配的可制热和制冷的温度控制单元。并使用Ionera公司的 MECA 4记录芯片涂抹磷脂并同时进行四个脂双层记录。.
With the temperature control unit, temperatures on the chip can be varied in between 0°C to 50°C, as it contains an active cooling and active heating function.
Temperature control of the Orbit mini: PEG induced current blockages of a alpha-hemolysin pore
(A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature.
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.
The image war kindly provided by Ionera Technologies GmbH
For fluorescence applications, the Orbit mini can be equipped with the "Fluorescence Microscopy Kit" which includes an adapted faraday shielding as well as "Meca 4 Recording Chips Fluo", four-well recording chips for fluorescence applications with 150 µm cavity size (Order # 132004).
Orbit mini EDR 软件
The software for the Orbit mini was developed by our partner Elements SRL (Italy). It is intuitive and easy to learn. Please watch this video on the Orbit mini to learn about the software:
Please download EDR Software Updates from the Elements s.r.l homepage, out partner for Orbit mini software tools. Click here for Software downloads.
MECA 4 记录芯片
The MECA recording substrate contains a 2 x 2 array of circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is formed by painting, with high success rates for functional bilayers. The MECA chip has been validated with a number of different ion channels including KcsA, gramicidin, α-hemolysin, KV1.3, NaV and many more.
The MECA recording chips are produced and quality assured by our partner Ionera Technologies GmbH in Freiburg Germany and shipped from Munich to our international customers. Different types of MECA recording chips are available which should be chosen depending on experiment.
Available chip types
- "Meca 4 Recording Chips 50 µm": Four-well recording chip with 50 µm cavity size (Order # 132001)
- "Meca 4 Recording Chips 100 µm": Four-well recording chip with 100 µm cavity size (Order # 132002)
- "Meca 4 Recording Chips 150 µm": Four-well recording chip with 150 µm cavity size (Order # 132003)
- "Meca 4 Recording Chips Fluo": Four-well recording chip for fluorescence applications with 150 µm cavity size (Order # 132004)