Orbit mini - 脂双层工作站
Orbit mini是一款小型的脂双层工作站，可同时记录四个人工脂双层。
Orbit mini的主要特点有：
- 到手可用、占地极小的独立系统
- 内置四通道低噪音放大器
- 可选的温度控制附件 - 自动制冷与加热
- 低噪音高带宽记录
- 通量提高到四通道
- 附带专用记录软件
- 目标蛋白可直接插入和通过蛋白脂质体插入
- 可记录: 电压、配体门控离子通道、温度敏感离子通道
- 也可记录纳米孔、抗菌肽、毒素等...
- 低实验成本的MECA芯片
Orbit mini包含一个内置的小型化四通道放大器，可在高带宽下进行低噪音记录而不需要其他设备辅助 – 可以在任何环境下进行实验。此外独特的设计使得测量室的温度可以自由控制而不会导入额外的噪音。
完整的Orbit mini平台包含内置四通道放大器(Elements s.r.l.)的记录单元和选配的可制热和制冷的温度控制单元。并使用Ionera公司的 MECA 4记录芯片涂抹磷脂并同时进行四个脂双层记录。.
更多信息:
附件与特征
温度控制单元
With the temperature control unit, temperatures on the chip can be varied in between 0°C to 50°C, as it contains an active cooling and active heating function.
Temperature control of the Orbit mini: PEG induced current blockages of a alpha-hemolysin pore
(A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature.
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.
The image war kindly provided by Ionera Technologies GmbH
Orbit mini荧光显像附件
For fluorescence applications, the Orbit mini can be equipped with the "Fluorescence Microscopy Kit" which includes an adapted faraday shielding as well as "Meca 4 Recording Chips Fluo", four-well recording chips for fluorescence applications with 150 µm cavity size (Order # 132004).
软件
Orbit mini EDR 软件
The software for the Orbit mini was developed by our partner Elements SRL (Italy). It is intuitive and easy to learn. Please watch this video on the Orbit mini to learn about the software:
Software 升级:
Please download EDR Software Updates from the Elements s.r.l homepage, out partner for Orbit mini software tools. Click here for Software downloads.
耗材
MECA 4 记录芯片
The MECA recording substrate contains a 2 x 2 array of circular microcavities in a highly inert polymer. Each cavity contains an individual integrated Ag/AgCl-microelectrode. The bilayer is formed by painting, with high success rates for functional bilayers. The MECA chip has been validated with a number of different ion channels including KcsA, gramicidin, α-hemolysin, KV1.3, NaV and many more.
The MECA recording chips are produced and quality assured by our partner Ionera Technologies GmbH in Freiburg Germany and shipped from Munich to our international customers. Different types of MECA recording chips are available which should be chosen depending on experiment.
Available chip types
- "Meca 4 Recording Chips 50 µm": Four-well recording chip with 50 µm cavity size (Order # 132001)
- "Meca 4 Recording Chips 100 µm": Four-well recording chip with 100 µm cavity size (Order # 132002)
- "Meca 4 Recording Chips 150 µm": Four-well recording chip with 150 µm cavity size (Order # 132003)
- "Meca 4 Recording Chips Fluo": Four-well recording chip for fluorescence applications with 150 µm cavity size (Order # 132004)
用户评价与案例分析
Prof. Dr. Chris Miller - Case Study about the Orbit mini
"Contrary to my initial expectations, the Orbit mini (red arrow) has performed exceptionally well for
our application, with vastly improved signal-tonoise characteristics over our best recordings on the home-built system."
“The compactness of the instrument makes for flexible and convenient placement in the lab. With a tiny footprint, it sits on a table or desk accompanied by only a laptop. In addition, bilayers are painted 'blind' with a teflon wand supplied by Nanion without observing the partition through a microscope. Thus, the
entire support-package of microscope, illuminator, Faraday cage, vibration-table, and rig-rack is eliminated. The acquisition software is intuitive and robust, and the data can be stored in formats readable by the Axon analysis programs that we routinely use. Moreover, the four electrically independent holes in the chip-partition means that four bilayers can be monitored simultaneously, thus quadrupling the probability of catching a clean single channel to record. This represents a significant, practical boost in experimental efficiency.”
Source: "Getting going with the Orbit mini planar bilayer system: A narrative"
From:
Prof. Dr. Chris Miller, Professor of Biochemistry, Investigator, Howard Hughes Medical Institute Member, US National Academy of Sciences, Brandeis University, Waltham, Massachusetts, USA
数据与应用
Alpha-Hemolysin - Temperature Control
Orbit mini and applications:
Data were kindly provided by Ionera.
Temperature control of the Orbit mini: PEG induced current blockages of a alpha-hemolysin pore
(A) Current traces recorded at 10°C and 40°C illustrating a strong increase of the open pore current as well as the blockage frequency at elevated temperature.
(B) Event averaged histograms of the residual current during blockages. The open pore current scales with the temperature as well as the dwell time of the blockages.
(C) Dependence of the open pore current on the temperature.
(D) Dependence of the frequency of blockages on the temperature.
OccK1 - Outer Membrane Protein of the Pathogenic Bacterium Pseudomonas Aeruginosa
Orbit mini and applications:
Data were kindly provided by Ionera.
Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram.
Conditions: 150 mM KCl, 5 mM MES, pH 6, 10 mM enrofloxacin, + 60 mV
OmpF - Current block
Orbit mini and applications:
Data were kindly provided by Ionera.
Representative recording from a single OmpF trimer showing current blocks due to the translocation of antibiotic Enrofloxacin, event averaged histogram.
Conditions: 150 mM KCl, 5 mM HEPES, 5 mM MES, pH 6, 10 mM enrofloxacin, +60 mV
TRPA1 - Temperature Dependency
Orbit mini data and applications:
(A) Effect of temperature on TRPA1 activity
(B) The open probability (Po) versus the temperature and fitted with Boltzmann equation (EC50 was found at 14˚C).
The Arrhenius plot of the same data resulted in a Q10 of 46 (Literature: Q10 ~ 40).
VDAC - Application of Fluoxetine
Orbit mini and applications:
Data were kindly provided by Ionera.
Current recordings of single VDA channels from selected bilayers in parallel.
VDAC was reconstituted in DPhPC membranes and partially blocked upon the addition of Fluoxetine (Prozac).
(A) Representative parallel recording from 3 VDAC channels in DPhPC membranes. Conditions: 1 M KCl, 25 mM Tris, pH 7,5, voltage change protocol is given below.
(B) Current trace illustration partial blocade of VDAC by Fluoxetine (Prozac).
(C) Current-voltage trace of a single VADC channel under a ramping voltage of -70/+70 mV before (green) and after (blue) addition of 50 µm Fluoxetine
Purified VDAC sample is a generous gift from Prof. Stephan Nussberger to Ionera, Department Biophysics, University of Stuttgart, Germany
网络研讨会与影像
Webinars
28.06.2018 | Webinar: Artificial Lipid Bilayers in focus: Hand-held DNA-sequencing and biosensing with nanopores
Orbit mini and 
Orbit 16
Learn about single channel measurements in bilayer recording using the orbit instrument family
- Simplifying artificial bilayer experiments: Single-molecule experiments on micro-cavity arrays
- Hand-held DNA-sequencing and biosensing with nanopores
27.01.2016 | Webinar: Instant bilayers - just add protein
Orbit 16 and Orbit Mini
This webinar covers the use of the lipid bilayer platforms from Nanion: the Orbit16 and the Orbit mini for characterization of membrane proteins like ion channels, bacterial porins and biological nanopores. Both bilayer systems support high quality low noise recordings, but differ in throughput capabilities and experimental features. The Orbit16, introduced in 2012 is a device for efficient formation of 16 lipid bilayers simultaneously, allowing for parallel bilayer-reconstitution of ion channels and nanopores.
Movies: Oral Presentations and Tutorials
2018 - Simplifying artificial bilayer experiments: Single-molecule experiments on micro-cavity arrays
Orbit 16 and Orbit mini Oral Presentation
Presenter:
Dr. Conrad Weichbrodt, Product Manager Orbit instrument family, Nanion Technologies GmbH, Germany
Source:
Webinar: "Artificial Lipid Bilayers in focus: Hand-held DNA-sequencing and biosensing with nanopores", June 28, 2018
2015 - Orbit mini Membrane Preparation Tutorial Video
Orbit Mini
Short introduction how to prepare membranes on the Orbit mini
下载:
应用数据
产品彩页
快速指南
Orbit mini - Quickstart Guide "Getting Started"
Orbit mini Quickstart Guide "Getting Started"
发表文献
2019 - Structure of the human ClC-1 chloride channel
Port-a-Patch, 
Vesicle Prep Pro and 
Orbit mini publication in PLOS Biology (2019)
Authors:
Wang K., Preisler, SS, Zhang, L., Cui, Y., Missel, JW., Grønberg C., Gotfryd, K., Lindahl E., Andersson, M., Calloe, K., Egea P.F., Klaerke D.A., Pusch M., Pedersen P.A., Zhou, Z.H., Gourdon, P.
2019 - Revisiting an old antibiotic: bacitracin neutralizes binary bacterial toxins and protects cells from intoxication
Orbit mini publication in The FASEB Journal (2018)
Authors:
Schnell L., Felix I., Müller B., Sadi M., von Bank F., Papatheodorou P., Popoff M.R., Aktories K., Waltenberger E., Benz R., Weichbrodt C., Fauler M., Frick M., Barth H.
2019 - Purification of Functional Human TRP Channels Recombinantly Produced in Yeast
Orbit mini publication in Cells (2019)
Authors:
Zhang L., Wang K., Klaerke D.A., Calloe K., Lowrey L., Pedersen P.A., Gourdon P., Gotfryd K.
2018 - Structures of monomeric and oligomeric forms of the Toxoplasma gondii perforin-like protein 1
Orbit mini and Vesicle Prep Pro publication in Science Advances (2018)
Authors:
Ni T., Williams S.I., Rezelj S., Anderluh G., Harlos K., Stansfeld P.J., Gilbert R.J.C.
2018 - Small Molecule Permeation Across Membrane Channels: Chemical Modification to Quantify Transport Across OmpF
Orbit mini pre-publication in ChemRxiv (2018)
Authors:
Wang J., Bafna J.A., Bhamidimarri S.P., Winterhalter M.
2018 - Functional Analysis of Membrane Proteins Produced by Cell-Free Translation
Port-a-Patch and Orbit mini article in Protein Engineering (2018)
Authors:
Dondapati S.K., Wüstenhagen D.A., Kubick S.
2018 - Dynamics and number of trans-SNARE complexes determine nascent fusion pore properties
Orbit mini publication in Nature (2018)
Authors:
Bao H., Das D., Courtney N.A., Jiang Y., Briguglio J.S., Lou X., Roston D., Cui Q., Chanda B., Chapman E.R.
2017 - Molecular determinants of permeation in a fluoride-specific ion channel
Orbit mini in eLife (2017)
Authors:
Last .B., Sun S., Pham M.C., Miller C.
2017 - Engineering a pH responsive pore forming protein
Orbit mini and 
Vesicle Prep Pro publication in Scientific Reports (2017)
Authors:
Kisovec M., Rezelj S., Knap P., Cajnko M.M., Caserman S., Flašker A., Žnidaršič N., Repič M., Mavri J., Ruan Y., Scheuring S., Podobnik M., Anderluh G.
2016 - Mechanistic signs of double-barreled structure in a fluoride ion channel
Orbit mini publication in eLIFE (2016)
Authors:
Last N.B., Kolmakova-Partensky L., Shane T., Miller C.
海报
2016 - Next level toxicity screening: From single channel to overall cell behavior
Orbit mini, 
CardioExcyte 96 and 
SyncroPatch 384PE (a predecessor model of the SyncroPatch 384i) poster, Meeting of the French Society of Toxinology (SFET) 2015 
(0.9 MB)
